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FIGURE: 1 / 2
Human IFN-gamma Protein (PHC4031) in Bioactivity
Immunofluorescence analysis of ZO-1 was performed using Caco-2 cells treated with 50 ng/mL of human IFN-gamma recombinant protein (Product # PHC4031) for 24 hours. The cells were labeled with anti-ZO-1 polyclonal antibody (Product # 40-2200) (Panel a & c : Green). The composite (b & d) represents the merged with DAPI (blue) and F-actin (red) staining. Cells treated with IFN-gamma displayed upregulation of Z... View More
Product Details
PHC4031
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human,
Rat
Expression System
E. coli
Amino acid sequence
QDPYVKEAEN LKKYFNAGHS DVADNGTLFL GILKNWKEES DRKIMQSQIV SFYFKLFKNF KDDQSIQKSV ETIKEDMNVK FFNSNKKKRD DFEKLTNYSV TDLNVQRKAI HELIQVMAEL SPAAKTGKRK RSQMLFQGRR ASQ
Molecular weight
16.9 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 0.5 - 3.0 ng/mL; determined by the dose-dependent cytostasis of human WiDr cells.
Conjugate
Form
Liquid
Purification
purified
Storage buffer
40mM tris, pH 7.4
Contains
no preservative
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Carrier-Free
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNg; IFNγ; Immune interferon; Interferon; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g; unnamed protein product
Gene Aliases: IFG; IFI; IFNG; IMD69
UniProt ID: (Human) P01579
Entrez Gene ID: (Human) 3458
Disclaimer
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