Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Dynabeads Magnetic Beads |
Dynabeads magnetic beads offer reliable and reproducible results through gentle and efficient magnetic bead-based separation, suitable for a wide range of applications including cells, T cells, proteins, and exosomes. These beads are highly cited within the research community, significantly contributing to advancements in life sciences, biotechnology, and clinical research. Supported by over 30 years of Dynabeads expertise, Thermo Fisher Scientific offers exceptional service and support for all Dynabeads products.
Dynabeads magnetic beads are superparamagnetic, uniform, and spherical particles used for the isolation, separation, and purification of various biological molecules and cells in both research and clinical research applications.
Scientists trust Dynabeads products for several reasons:
The Dynabeads magnetic separation protocol generally consists of three simple steps: bind, wash, and release. When selecting Dynabeads products, there are two main considerations: bead size and coating. Dynabeads magnetic beads come in several sizes and surface functionalities, making them suitable for a wide range of applications. Some beads are precoupled with a biomolecule (ligand), which can be an antibody, protein, antigen, DNA/RNA probe, or any other molecule with an affinity for the desired target. Ready-to-use Dynabeads products and kits are available for many applications, and for assays that require flexibility, a range of Dynabeads products with specific characteristics are available, helping ensure that users can find the right beads for their specific needs.
Learn more about application areas and how Dynabeads products can help your research.
Streptavidin is a protein with high affinity for biotin, used in various molecular applications. Dynabeads streptavidin magnetic beads are used in the isolation of cells, isolation of proteins, nucleic acid capture, mRNA synthesis, and NGS workflows.
Immunoprecipitation is a technique used to isolate a specific antigen from a mixture. Dynabeads magnetic beads offer high capacity and yield, low non-specific binding, sensitivity, reproducibility, and cost effectiveness.
Cell isolation is a process used to separate specific cell types from a population of interest. Dynabeads are coated with antibodies or ligands for positive cell isolation, negative cell isolation, or cell depletion from various sample types.
Surface-activated magnetic beads are used to immobilize a target ligand for protein capture from a sample. Dynabeads magnetic beads with different surface functionalities, such as tosylactivated, carboxylic acid, amine, or epoxy are available for isolation of proteins, target nucleic acids, and cells.
T cell activation and expansion are crucial for immune response and therapy. Dynabeads products can help activate and expand naïve and memory T cells, T cell clones, regulatory T cells, natural killer T (NKT) cells, and cytotoxic T lymphocytes (CTLs) from mouse and human samples.
Learn more about T cell activation and expansion magnetic beads
Exosome research involves studying small extracellular vesicles for their role in cell communication and health. Dynabeads facilitate efficient exosome isolation in various research applications.
mRNA synthesis involves creating messenger RNA for various applications. Dynabeads enable a scalable magnetic bead-based method for mRNA synthesis and purification, enabling template reuse for in vitro transcription and a bind-wash-elute workflow for mRNA purification.
Learn more about mRNA synthesis and purification mgnetic beads
Viral enrichment is crucial for research studies as it allows the purification and concentration of viruses present in low concentrations. Dynabeads magnetic beads support a rapid and convenient method for isolating negatively charged viruses, helping ensure high yield and preserving their integrity.
Nucleic acid isolation and purification is commonly the first step in a molecular biology lab. MagMAX kits provide an efficient and reliable method for the purification and extraction of DNA and RNA from various sample types using magnetic beads.
Increase efficiency by removing manual steps and reducing overall processing time, maintain consistent results with every run, enjoy easy installation (ready to run in 10 minutes or less), and benefit from optimized, easy-to-follow protocols by downloading existing protocols from our library.
Learn more about KingFisher instruments for automated protocols
The Dynabeads magnetic separation protocol generally consists of three simple steps. No centrifugation steps or columns are required for sample processing, and the protocol can be run manually with Invitrogen DynaMag magnetic racks or automated on Thermo Scientific KingFisher instruments.
Figure 1. Dynabeads work in three steps: bind, wash, and release.
Dynal is built on a breakthrough that revolutionized the separation of biological materials. In 1976, the Norwegian professor John Ugelstad first succeeded in making spherical polystyrene beads of uniform size—only previously achieved by NASA in the weightless conditions of space. Another amazing achievement followed when the uniform beads were made magnetizable.
As the first mono-disperse solid-phase in solution, these pioneering innovations defined magnetic separation technology and allowed researchers to get lab results that were previously unattainable. A wide range of easy-to-use Dynabeads magnetic beads have since been developed, creating unlimited possibilities for biological discovery. Over the years, this Dynabeads magnetic bead technology has been used in a staggering range of applications within life sciences, biotech, and healthcare.
Exceptional product quality and reproducibility has always been the hallmark of Dynal. Inspired by the Norwegian invention of magnetic bead-based separation technology, we are committed to helping deliver consistency and reduce variability in your protocols.
There are two main considerations for Dynabeads products—bead size and coating. Many optimized Dynabeads magnetic beads are ready for immediate use in a variety of applications. Alternatively, you can easily prepare magnetic beads with your own antibody, oligonucleotide, or other ligand of choice.
There are a multitude of ways to use Dynabeads magnetic beads for cell, protein, ligand, or nucleic acid isolation. View the table below to determine which Dynabeads product will work best with your starting sample and downstream application.
Type | Product | Starting sample | Benefits |
---|---|---|---|
Streptavidin | Dynabeads FlowComp Flexi Kit | PBMC, blood, single cell suspensions from lymph nodes, spleen, or other tissues | This kit facilitates everything you need for labeling of antibodies and subsequent cell isolation. Beads are removed after isolation to obtain pure and viable cells suitable for any downstream application. |
CELLection Biotin Binder Kit | PBMC, tissue digests, blood | Use with any biotinylated antibody to positively isolate cells from any species. The beads are removed after isolation to obtain pure and viable cells for use in any application. | |
Dynabeads Biotin Binder | PBMC, tissue digests, blood | Isolate any cell from any species. This kit binds all biotinylated antibodies and is our most flexible secondary product for cell isolation or depletion. | |
Dynabeads Sheep anti-Rat IgG | PBMC, tissue digests, blood | Isolate any cell type from any species excluding rat to use with your preferred primary IgG rat antibody. Excellent for positive isolation, negative isolation, or depletion of cells. | |
Secondary-coated | Dynabeads Pan Mouse IgG | PBMC, tissue digests, blood | A flexible secondary product for cell isolation or depletion. It will bind all subclasses of mouse IgG and is Fc specific. Requires less antibody and has shown no cross reactivity to human, rat, hamster, goat, rabbit, and guinea pig IgG. |
CELLection Pan Mouse IgG | PBMC, tissue digests, blood | Use with any mouse IgG to positively isolate cells from any species except mouse. Beads can be removed after isolation to leave pure and viable cells for use in any application. | |
Dynabeads Sheep anti-Mouse IgG | PBMC, tissue digests, blood | Isolate any cell type from any species except mouse. For use with your primary mouse IgG1, IgG2a, or IgG2b antibody of choice. Excellent for positive isolation, negative isolation, or depletion of cells. Also available in clinical research grade as Dynabeads ClinExVivo Sheep anti-Mouse IgG. | |
Dynabeads Goat anti-Mouse IgG | PBMC, tissue digests, blood | Isolate any cell type from any species except mouse. For use with your primary IgG mouse antibody of choice. Exceptional for positive isolation, negative isolation, or depletion of cells. | |
Dynabeads Rat anti-Mouse IgM | PBMC, tissue digests, blood | Isolate any cell type from any species except mouse. For use with your primary mouse IgM antibody of choice. Exceptional for positive isolation, negative isolation, or depletion of cells. | |
Dynabeads Sheep anti-Rabbit IgG | PBMC, tissue digests, blood | Isolate any cell type from any species except rabbit. For use with your primary rabbit antibody of choice. This product can be used for eukaryotic cells. | |
Surface-activated | Dynabeads M-450 Epoxy | PBMC, tissue digests, blood | Hydrophobic naked beads with glycidyl ether (epoxy) reactive groups. Excellent for coupling of antibodies for cell applications. Coupling reactions can be performed at neutral pH, with 24-hour incubation at room temperature. Also available in clinical research grade as Dynabeads ClinExVivo Epoxy. |
Dynabeads M-450 Tosylactivated | PBMC, tissue digests, blood | Hydrophobic naked beads with p-toluenesulphonyl (tosyl) reactive groups. For coupling of antibodies for cell applications. Coupling reactions can be performed at neutral pH although high pH and incubation at 37°C will promote covalent binding. |
DynaGreen Magnetic Beads enable high-quality, reproducible results and are also more sustainable and microplastic-free. How do these beads contribute to more sustainable science? All DynaGreen Magnetic Beads have the following features:
View Dynabeads-related products and accessories available for use with our magnetic beads.
DynaMag magnetic racks are used to isolate any target in combination with Dynabeads magnetic beads. These magnets help ensure optimal working positions and are functionally adapted to suit your workflow.
The HulaMixer Sample Mixer is a tube rotator and rotating mixer. Samples often need to incubate with Dynabeads magnetic beads so appropriate binding can occur.
Dynabeads magnetic beads and kits are validated for automated workflows on KingFisher Sample Purification Systems.
Automation systems are available for your workflows with Dynabeads magnetic beads and kits. KingFisher instruments support software for automated protocols.
Have a question about a Dynabeads product? Our technical specialists can help! Ask us
Additional publications can be found in our Citations and References database. The database can be searched using a product catalog number or keyword. For example, searching with the term "10001D" will return a list of journal articles that cite the use of Dynabeads Protein A for Immunoprecipitation (Cat. No. 10001D) and searching with the term "dynabeads" will return a list of journal articles that cite the use of any Dynabeads product. Note that the Citations and References database on thermofisher.com contains only the small subset of scientific journal articles that cite the use of our products. Many more can be found via targeted searches of the PubMed™ database and similar databases.
For Research Use Only. Not for use in diagnostic procedures.