Dynabeads™ Sheep anti-Rat IgG
Dynabeads™ Sheep anti-Rat IgG
Invitrogen™

Dynabeads™ Sheep anti-Rat IgG

The product can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed.
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Catalog NumberQuantity
110355 mL
11036D2 mL
Catalog number 11035
Price (USD)/ Each
864.00
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Quantity:
5 mL
Price (USD)/ Each
864.00
Add to cart
Dynabeads™ Sheep anti-Rat IgG
Catalog number11035
Price (USD)/ Each
864.00
-
Add to cart
Dynabeads Sheep Anti-Rat IgG are 4.5 μm superparamagnetic beads with affinity purified polyclonal sheep anti-rat IgG covalently bound to the bead surface. These beads can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed. Dynabeads Sheep Anti-Rat IgG are especially well suited for cell isolation from mouse samples (cross-reactivity to mouse antibodies is high and cross-reactivity to human antibodies is minimal).

Advantages of Dynabeads Sheep Anti-Rat IgG:

  • Works with any cell type, from any sample and from any species (except rat)
  • Fast and efficient depletion or positive isolation of any target cell for molecular downstream assays using rat primary antibody
  • Obtain pure and untouched cells using a mixture of rat IgGs to deplete unwanted cells

Magnetic bead-based separation offers easy handling

The rat primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads Sheep Anti-Rat IgG (direct technique). Regardless of which technique is used, when the cell sample and the Dynabeads Sheep Anti-Rat IgG are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more rat primary antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for molecular downstream experiments (positive isolation). For positive isolation of bead-free cells (e.g., for flow cytometry), we recommend using your rat antibody in combination with the Dynabeads FlowComp Flexi Kit.

Useful for many sample types

Using Dynabeads Sheep Anti-Rat IgG, you can isolate cells from many starting samples including whole blood, mouse spleen or lymph node cells, and tissue digests.

Learn more about Dynabeads products

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibody FragmentWhole Antibody
Cell TypeAll cells from all species, except rat
ClonalityPolyclonal
Product TypeAntibody Coated Bead
Dye TypeDynabeads™
Host SpeciesSheep
Includes5 mL of Dynabeads Sheep Anti-Rat IgG (4 x 108 Dynabeads/mL) in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide as a preservative.
Isolation TechnologyDepletion, negative isolation or positive isolation
No. of CellsProcesses ∼2 x 109 cells total
Output Viability>95%
Product LineDYNAL™, Dynabeads™
Purity or Quality GradeResearch Grade
Quantity5 mL
ReactivityAll species, except rat
Sample TypePBMC, Tissue Digests, Blood
Shipping ConditionRoom Temperature
Starting Material Cell No.1 x 107 PBMCs per isolation
Target SpeciesAll species, except rat
Diameter (Metric)4.5 μm
Unit SizeEach
Contents & Storage
This product contains 5 mL of Dynabeads™ Sheep Anti-Rat IgG (4 x 108 Dynabeads™/mL) in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide.

Store at 2°C to 8°C.