Dynabeads® Sheep Anti-Rat IgG

Catalog number: 11035

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Dynabeads® Sheep Anti-Rat IgG are 4.5 µm superparamagnetic beads with affinity purified polyclonal sheep anti-rat IgG covalently bound to the bead surface. These beads can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed. Dynabeads® Sheep Anti-Rat IgG are especially well suited for cell isolation from mouse samples (cross-reactivity to mouse antibodies is high and cross-reactivity to human antibodies is minimal).

• Works with any cell type, from any sample and from any species (except rat)
• Fast and efficient depletion or positive isolation of any target cell for molecular downstream assays using rat primary antibody
• Obtain pure and untouched cells using a mixture of rat IgGs to deplete unwanted cells

Magnetic bead-based separation offers easy handling
The rat primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads® Sheep Anti-Rat IgG (direct technique). Regardless of which of these techniques is used, when the cell sample and the Dynabeads® Sheep Anti-Rat IgG are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more rat primary antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for molecular downstream experiments (positive isolation). For positive isolation of bead-free cells (e.g., for flow cytometry), we recommend using your rat antibody in combination with the Dynabeads® FlowComp™ Flexi Kit.

Useful for many sample types
Using Dynabeads® Sheep Anti-Rat IgG, you can isolate cells from many starting samples including whole blood, mouse spleen or lymph node cells, and tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~2x10^9 cells total
Cell Type: All cells from all species, except rat
Clonality: Polyclonal
Reactivity: All species, except rat
Host Species: Sheep
Label or Dye: Dynabeads®
Product Size: 5 mL
Target Species: All species, except rat
Isolation Method: Depletion, negative isolation or positive isolation
Output Viability: >95%
Antibody Fragment: Whole Antibody
Sample Type (Specific): Blood, Tissue Digests, PBMC
Starting Material (Cell #): 1x10^7 PBMCs per isolation
Shipping Condition: Room Temperature

Contents & storage

This product contains 5 mL of Dynabeads® Sheep Anti-Rat IgG (4 × 10^8 Dynabeads®/mL) in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide as a preservative. Store at 2–8°C.

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