Dynabeads® Biotin Binder

Catalog number: 11047

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Dynabeads® Biotin Binder are 2.8 µm superparamagnetic beads that bind biotinylated antibodies (or other biotinylated ligands), allowing you to isolate cells from any species (e.g., human, mouse, rat, non-human primates, and cell lines).

• Works with any cell type, from any sample and from any species
• Fast and efficient depletion or positive isolation of any target cells for molecular downstream assays using a biotinylated primary antibody
• Obtain pure and untouched cells using a mixure of biotinylated antibodies to deplete unwanted cells

Adaptable for various isolation strategies
The biotinylated primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads® Biotin Binder (direct technique). Regardless of which of these techniques is used, when the cell sample and the Dynabeads® Biotin Binder are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more biotinylated antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for downstream experiments (positive isolation). For isolation of bead-free cells (e.g., for flow cytometry), we recommend using your biotinylated antibody in combination with the CELLection™ Biotin Binder Kit.

Useful for many sample types
Using Dynabeads® Biotin Binder, you can isolate cells basically from any species, and from many starting samples such as whole blood, cord blood, peripheral blood mononuclear cells (PBMC), buffy coat, mouse spleen/lymph nodes, and tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~2x10^9 cells total
Cell Type: All cells from all species
Reactivity: All species
Product Size: 5 mL
Target Species: All species
Isolation Method: Depletion, negative isolation or positive isolation
Output Viability: >95%
Sample Type (Specific): Blood, Tissue Digests, PBMC
Starting Material (Cell #): 1x10^7 PBMCs per isolation
Shipping Condition: Room Temperature

Contents & storage

This Product Contains: 5 ml Dynabeads® coated with recombinant streptavidin.
Store at 2-8°C.

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Manuals & protocols

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