Dynabeads® M-280 Sheep Anti-Rabbit IgG

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Description

Dynabeads® M-280 Sheep anti-Rabbit IgG are 2.8 µm superparamagnetic beads with affinity purified polyclonal sheep anti-rabbit IgG covalently bound to the bead surface. These beads are designed to serve as a solid support for simple and efficient binding of rabbit IgG subclasses and their target proteins.

• Fast and easy coupling and isolation procedure
• No columns or centrifugation steps required
• Binds most rabbit IgG subclasses

Magnetic bead-based separation offers easy handling
The primary antibody that recognizes the target molecule may be added to the sample (indirect technique) or pre-coated onto the Dynabeads® M-280 Sheep anti-Rabbit IgG (direct technique). Regardless of which of these techniques is used, when the Dynabeads® M-280 Sheep anti-Rabbit IgG are mixed with the sample, the beads bind to the target. Placing the sample on a magnet separates the bead-bound target from the rest of the sample. The supernatant is then removed by aspiration. The target molecule can be eluted off the beads with conventional elution methods, or used in further downstream applications while still attached to the beads.

Adaptable for various applications
Dynabeads® M-280 Sheep anti-Rabbit IgG are uniform and spherical, monodisperse, nonporous beads, making them an ideal choice for applications such as binding Ig, protein purification, sandwich immunoassays, IP, Co-IP, and isolation of cells and microorganisms. They are highly mobile in solution enabling the capture surface to freely and continuously interact with the entire sample volume.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~2x10^9 cells total
Cell Type: All cells from all species, except rabbit
Clonality: Polyclonal
Reactivity: All species, except rabbit
Ligand Type: Antibody
Host Species: Sheep
Label or Dye: Dynabeads® M-280
Product Size: 2 mL
Concentration: 10 mg⁄ml
Isolation Method: Depletion, negative isolation or positive isolation
Output Viability: >95%
Antibody Fragment: Whole Antibody
Shipping Condition: Room Temperature

Contents & storage

Contains: 2ml (10 mg/ml concentration) Dynabeads® M-280 Sheep anti-Rabbig IgG
Storage: 2°C to 8°C

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Manuals & protocols

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