Fluorescence Imaging Information

Fluorescence imaging is a powerful tool for researchers but requires a significant amount of knowledge and skill to get accurate results. Here you will find a wide variety of tools to help you get the most out of your experiments.

Fluorescence imaging features

On-demand webinar

Fixed cell imaging—Five steps for publication-quality images

With over 40 years dedicated to cell imaging research, we offer long-proven tools and protocols to help confidently create quality cell images the first time. This on-demand webinar covers the 5 essential steps to getting great images.

 

eBook

eBook

Live cell imaging

Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live-cell imaging. Content includes basic history of live-cell imaging, case studies of live-cell imaging applications, and common problems and solutions for live-cell imaging.

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Fluorescence imaging learning resources

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Type Title Categories
Application note (2012) Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening  ArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability
Application note (2013) Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader: Stem cell colonies  Alexa Fluor, antibodies, ArrayScan, confocal microscopy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, immunofluorescence (IF), microplate reader, particles, stem cell research
Application note (2013) Multiplexed mitosis and apoptosis analysis  antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles
Application note (2014) High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry  ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Application note (2014) Elucidating the mechanism of cell death through the use of high content analysis  apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Application note (2015) Using time-lapse imaging with the EVOS FL Auto Imaging System EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator
Application note (2015) Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with Onstage Incubator EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator, phagocytosis
Application note (2015) Quantitation of proliferating cells with the EVOS FL Auto Imaging System antibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis
Application note (2015) Phagocytosis visualized on the EVOS FL Auto Imaging System with Onstage Incubator CellLight, EVOS, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes
Application note (2015) Image tiling and stitching using the EVOS FL Auto Imaging System cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, Imaging sample preparation, ReadyProbes
Application note (2015) Collecting Z-stack image sequences with the EVOS FL Auto Imaging System cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes
Application note (2015) Cell division and migration during wound healing visualized on the EVOS FL Auto Imaging System cell health, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging
Application note (2017) Simple and accurate monitoring of adipogenesis adipocyte, adipogenesis, automated cell counter, cell differentiation, Countess, EVOS, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator, stem cell research
Application note (2017) Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes antibodies, Attune/Attune NxT, cardiomyocytes, cell differentiation, flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunophenotyping, stem cell research
BioProbes article (2006) Analyzing cell culture health and concentration  apoptosis, cell health, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability
BioProbes article (2006) Examining the role of developmental asymmetry in the formation of heart defects  Alexa Fluor, cardiovascular system, cell differentiation, cell tracking, fluorescent dyes, fluorescence microscopy/fluorescence imaging
BioProbes article (2006) Injectable contrast reagents for in vivo imaging  Alexa Fluor, cancer, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging
BioProbes article (2006) Tools for membrane research  cell structure-mitochondria, cell structure-plasma membrane, cell tracking, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, lipids
BioProbes article (2006) Invitrogen flow cytometry—building choice  cell culture, cell cycle, cell health, cell isolation, cell signaling, flow cytometer/flow cytometry, fluorescent dyes, Gibco, fluorescence microscopy/fluorescence imaging, nanocrystals, protein labeling, Qdot, Zenon
BioProbes article (2006) Products for neurobiology research  enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in situhybridization, nanocrystals, protein detection, protein labeling, Qdot, viability, Zenon
BioProbes article (2006) Reagents and assays for high-content screening  cell structure-nucleus, cell structure-plasma membrane, fluorescent dyes, high content analysis, fluorescence microscopy/fluorescence imaging
BioProbes article (2006) A guide to antibody labeling and detection  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), multicolor flow cytometry, nanocrystals, Qdot, Zenon
BioProbes article (2007) Staining bacterial biofilms: new uses for classic fluorescent dyes  biofilm staining, CellTrace, enzyme activity detection, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microbiology, viability
BioProbes article (2007) The TC-FlAsH TC-ReAsH protein detection choice  fluorescent dyes, fluorescence microscopy/fluorescence imaging, live-cell imaging, protein detection, protein labeling, stem cell research
BioProbes article (2007) O-GlcNAc: Hiding in plain sight  Click-iT, fluorescent dyes, fluorescence microscopy/fluorescence imaging, protein detection, protein labeling, western blotting
BioProbes article (2007) Seeing superoxide in a radical new light  cell structure-mitochondria, fluorescent dyes, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress, sample stability
BioProbes article (2007) Effortless protein conjugations for small animal in vivo imaging  Alexa Fluor, antibody labeling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging
BioProbes article (2007) Important additions to the Alexa Fluor dye series  Alexa Fluor, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, protein labeling, sample stability
BioProbes article (2007) The latest in stem cell research  antibodies, antibody labeling, cell differentiation, cell expansion, flow cytometer/flow cytometry, fluorescent dyes, growth factors, fluorescence microscopy/fluorescence imaging, stem cell research
BioProbes article (2007) Mitochondria and cardiac disorders  antibodies, antibody labeling, cardiovascular system, cell structure-mitochondria, fluorescent dyes, fluorescence microscopy/fluorescence imaging, oxidative stress, western blotting
BioProbes article (2007) How much DNA, RNA, or protein is really in your sample?  fluorescent dyes, fluorometer, fluorescence microscopy/fluorescence imaging, microplate reader, nucleic acid quantitation, protein quantitation, Qubit
BioProbes article (2007) Breakthrough cell proliferation assay for flow cytometry  Alexa Fluor, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid quantitation
BioProbes article (2007) Fluorescent calcium indicators for in vivo imaging of neuronal networks  cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging, ion indicators, neuroscience, two-photon microscopy
BioProbes article (2007) Cells from the inside out  cell structure-all, flow cytometer/flow cytometry, fluorescent proteins, fluorescence microscopy/fluorescence imaging, in vivo imaging, multicolor flow cytometry, nanocrystals, Qdot
BioProbes article (2007) Phospholipase activity detection from A to D  enzyme activity detection, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader
BioProbes article (2007) Improved results are just a click away  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, multicolor flow cytometry, nucleic acid quantitation, nucleosides
BioProbes article (2007) Putting the pH in phagocytosis  flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, multicolor flow cytometry, live-cell imaging, pH detection, phagocytosis, reagents, sample preparation
BioProbes article (2007) New applications for Vybrant DyeCycle stains  Alexa Fluor, cell cycle, cell isolation, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, multicolor flow cytometry, stem cell research
BioProbes article (2007) TC-FlAsH and TC-ReAsH reagents  fluorescent dyes, fluorescence microscopy/fluorescence imaging, live-cell imaging, protein labeling
BioProbes article (2007) Highly sensitive methods for phosphate detection  colorimetric, enzyme activity detection,fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, sample preparation
BioProbes article (2007) Optimally labeled fluorescent antibodies for in vivo animal imaging  Alexa Fluor, antibody labeling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging
BioProbes article (2007) Following differentiation into adipocytes  cell differentiation, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, stem cell research
BioProbes article (2007) Fluorescent probes for two-photon microscopy  Alexa Fluor, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging, neuroscience, two-photon microscopy
BioProbes article (2007) in situ hybridization made easy  Alexa Fluor, brightfield microscopy,  fluorescent dyes, fluorescence microscopy/fluorescence imaging, gene expression, in situhybridization, nucleic acid detection, sample stability
BioProbes article (2007) Click. Discover. Publish  Click-iT, fluorescent dyes, fluorescence microscopy/fluorescence imaging, protein detection, protein labeling, western blotting
BioProbes article (2007) Listening in on cellular communication  cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, ion indicators, microplate reader
BioProbes article (2008) Signal preservation for live- and fixed-cell analysis  Alexa Fluor, antifades, fluorescent dyes, fluorescence microscopy/fluorescence imaging, live-cell imaging, nanocrystals, Qdot, sample stability
BioProbes article (2008) Cell proliferation studies made simple  Alexa Fluor, cell proliferation, Click-iT, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleosides, sample stability
BioProbes article (2008) The picture of cell health  Alexa Fluor, cell health, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, nucleic acid quantitation, nucleosides, reagents
BioProbes article (2008) Click chemistry: Making biodiscovery a snap  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, nucleosides
BioProbes article (2008) Qdot 625 nanocrystals and conjugates  flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, multicolor flow cytometry, nanocrystals, Qdot
BioProbes article (2008) Potassium flux assay meets BacMam technology  BacMam technology, cell signaling, fluorescent dyes, genes, fluorescence microscopy/fluorescence imaging, ion flux/ion channels, ion indicators, microplate reader, reagents
BioProbes article (2008) Fluorescent protein–based Organelle Lights and new Cellular Lights BacMam reagents  BacMam technology, cell structure-all, cell tracking, Cellular Lights, fluorescent proteins, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC)
BioProbes article (2008) IgG antibody labeling from A to Z—Alexa Fluor to Zenon IgG Antibody Labeling Kits for imaging and flow cytometry  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), protein labeling, Zenon
BioProbes article (2008) Seeing green—Alexa Fluor 488 conjugates outshine the rest  Alexa Fluor, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample stability
BioProbes article (2008) Analyzing signaling events with phosphorylation site–specific antibodies—Insights into functional proteomics  antibodies, antibody labeling, cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), protein labeling
BioProbes article (2008) Seeing clearly: Accurate cell identification—CellMask stains for fluorescence-based imaging  cell structure-cytoplasm, cell structure-nucleus, cell structure-plasma membrane, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging
BioProbes article (2008) Measuring mitochondrial protein levels and enzyme activity—Get both readouts with MitoProfile dipstick and microplate assays  enzyme activity detection, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, protein detection
BioProbes article (2009) Novel recombinant monoclonal antibodies—ABfinity technology ensures performance  antibodies, cell signaling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), protein quantitation, sample preparation, western blotting
BioProbes article (2009) Modeling angiogenesis in vitro  Alexa Fluor, angiogenesis, cell culture, cell proliferation, Click-iT, fluorescent dyes, fluorescence microscopy/fluorescence imaging, membranes
BioProbes article (2009) Fluorescence-based alternatives to chromogenic immunohistochemistry  antifades, cancer, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), particles, protein quantitation, sample stability
BioProbes article (2009) Multiparametric approaches for predictive hepatotoxicity  cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress, viability
BioProbes article (2009) Beyond Classification—Click-iT EDU outperforms BRDU across all species  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid detection, nucleic acid quantitation, nucleosides, sample preparation
BioProbes article (2009) Highly specific antibodies for cell junction studies  Alexa Fluor, antibodies, cell junction studies, ELISA, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), particles, western blotting
BioProbes article (2009) Nanotechnology meets cellular analysis  cell structure-all, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, media, multicolor flow cytometry, nanocrystals, Qdot, sample stability
BioProbes article (2009) Visualizing tumor metastasis  cancer, cell tracking, CellTrace, enzyme activity detection, fluorescent dyes, fluorescence microscopy/fluorescence imaging, live-cell imaging, membranes
BioProbes article (2009) Innovative solutions for ion channel research  BacMam technology, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, ion indicators, microplate reader
BioProbes article (2009) A fluorogenic, target-specific reagent for small animal in vivo imaging  Alexa Fluor, antibody labeling, cancer, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging, protein labeling, sample preparation
BioProbes article (2009) RNA as you've never seen it before  Alexa Fluor, Click-iT, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, nucleic acid detection, nucleosides
BioProbes article (2009) Live-cell imaging of cell cycle and cell division  BacMam technology, cell cycle, flow cytometer/flow cytometry, fluorescent proteins,  fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging
BioProbes article (2009) Mitochondrial fission and fusion in neurodegeneration  antibodies, BacMam technology, cell structure-mitochondria, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, fluorescence microscopy/fluorescence imaging, microplate reader, neuroscience
BioProbes article (2009) Interrogating potential drug targets for Parkinson's disease  antibodies, cell signaling, enzyme activity detection, fluorescence microscopy/fluorescence imaging, neuroscience, proteins, substrates
BioProbes article (2009) Neurons in focus: Two-photon excitation imaging in live neuronal tissue  Alexa Fluor, fluorescent dyes, fluorescent proteins, fluorescence microscopy/fluorescence imaging, ion flux/ion channels, ion indicators, live-tissue imaging, nanocrystals, neuroscience, Qdot, two-photon microscopy
BioProbes article (2009) Improve your image—Two easy steps to reduce background noise and preserve signal strength  antifades, fluorescence microscopy/fluorescence imaging, reagents, sample preparation, sample stability
BioProbes article (2009) The envelope, please: Best picture for biofilms  biofilm staining, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microbiology, microplate reader, viability
BioProbes article (2009) Specific detection of gap junction proteins  Alexa Fluor, antibodies, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), protein detection
BioProbes article (2009) High-content analysis of cytotoxicity  cell health, cell proliferation, Click-iT, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, viability
BioProbes article (2009) A pHrodo dye-based method for monitoring the phagocytic internalization of apoptotic cells  apoptosis, fluorescent dyes, fluorescence microscopy/fluorescence imaging, pH detection, phagocytosis
BioProbes article (2009) Lights. Cells. Action. Organelle Lights and Cellular Lights reagents   BacMam technology, cell structure-all, Cellular Lights, fluorescent proteins, fluorescence microscopy/fluorescence imaging, live-cell imaging
BioProbes article (2009) Faster, easier calcium flux assays  cell signaling, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, microplate reader
BioProbes article (2009) Near-infrared dye for live cell cycle analysis  cell cycle, flow cytometer/flow cytometry, fluorescent dyes, live-cell imaging, nucleic acid detection
BioProbes article (2009) Modeling mammary cell function in three dimensions  BacMam technology, cell culture, cell structure-actin, cell structure-golgi, Cellular Lights, fluorescent proteins, Gibco, fluorescence microscopy/fluorescence imaging, membranes, particles, sample preparation
BioProbes article (2009) High-throughput chloride channel screening  BacMam technology, fluorescent proteins,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, ion indicators
BioProbes article (2009) TUNEL vision  apoptosis, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, sample preparation, sample stability
BioProbes article (2009) Fluorescence technologies for high-content imaging and analysis  Alexa Fluor, cell health, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress, protein detection, viability
BioProbes article (2009) Novel solid-phase antibody labeling  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample preparation, Zenon
BioProbes article (2009) Staining DNA without altering its structure  cell structure-nucleus, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, live-cell imaging, nucleic acid detection
BioProbes article (2010) Putting autophagy into context autophagy, CellInsight fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2010) Biology in context—Transforming live-cell microscopy with CellLight reagents cell structure, CellLight fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, live-cell imaging
BioProbes article (2010) Choosing the right dye for imaging intracellular calcium calcium flux, fluorescence microscopy/fluorescence imaging, high content analysis, ion channels, ion flux, ion indicators, reagents, sample preparation
BioProbes article (2010) Highly selective antibodies for GFP and RFP antibodies, fluorescence microscopy/fluorescence imaging, fluorescent proteins
BioProbes article (2010) Probes for visualizing secretory transport and autophagy autophagy, cell structure, cell structure-ER, cell structure-golgi, cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2010) Cells under stress—Fluorescence-based tools for investigating autophagy apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2010) Alexa Fluor dyes shed light on a new actin assembly factor cell structure-actin, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2011) Tali Image-Based Cytometer—Benchtop GFP/RFP transfection and viability determination has arrived fluorescence microscopy/fluorescence imaging, fluorescent proteins, viability
BioProbes article (2011) Neurons in living color—BacMam 2.0 technology and other imaging tools for live-cell studies CellLight, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, live-cell imaging, neuroscience
BioProbes article (2011) Tracking cells through time and space—Choose from fluorescent dyes, Qdot nanocrystals, and targeted fluorescent proteins CellTrace, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging
BioProbes article (2011) SlowFade Gold Antifade: A glycerol-based mountant for Immediate viewing—Keep photobleaching at bay fluorescence microscopy/fluorescence imaging, imaging sample preparation
BioProbes article (2011) Detection of actin, intermediate filaments, and tubulin—Imaging a dynamic cellular scaffold cell structure, cell structure-actin, CellLight fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2011) Click-iT Labeling Kits detect biomolecules across multiple applications—Biological discovery is a click away cell proliferation, Click-iT EdU, fluorescence microscopy/fluorescence imaging
BioProbes article (2011) Fluorescence imaging of oxidative stress in live cells—CellROX Deep Red Reagent is a bright and photostable ROS sensor CellROX, fluorescence microscopy/fluorescence imaging, oxidative stress
BioProbes article (2011) Bringing cell death to light—CellEvent Caspase-3/7 Substrate and the multiplex analysis of apoptosis apoptosis, ArrayScan, CellEvent, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
BioProbes article (2011) Advances in high-content screening—Assays for multiparametric analysis of cell health ArrayScan, cell health, cell proliferation, cell structure-plasma membrane, Click-iT EdU, fluorescence microscopy/fluorescence imaging, high content analysis, viability
BioProbes article (2011) Explore the powerhouse—Probes for mitochondrial morphology and function cell structure, cell structure-mitochondria CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2011) Focusing on the FAK signaling pathway—Phosphorylation-specific antibodies for Focal Adhesion Kinase (FAK) antibodies, cell signaling, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC)
BioProbes article (2011) Pushing the limits of fluorescence microscopy—Fluorescent probes for super-resolution imaging technologies fluorescence microscopy/fluorescence imaging, fluorescent dyes, SIM, STED, STORM, super resolution microscopy
BioProbes article (2011) Endocytosis in disease and cell health cell health, cell structure, CellLight, endocytosis and phagocytosis, fluorescence microscopy/fluorescence imaging, high content analysis, pH detection
BioProbes article (2011) Shedding light on oxidative stress—CellROX Deep Red Reagent for ROS detection ArrayScan, cell health, CellROX, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2011) No-wash imaging of apoptosis—CellEvent Caspase-3/7 Green Detection Reagent for apoptosis apoptosis, ArrayScan, caspase substrates, CellEvent, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2012) Expanding the fluorescence toolbox—Improved Qdot probes make multiplexing even easier flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, multicolor flow cytometry, particles, Qdot
BioProbes article (2012) Tracking internalization in live cells with pHrodo dyes endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, internalization, live-cell imaging, pHrodo
BioProbes article (2012) Streamline your oxidative stress measurements—CellROX Green and CellROX Orange reagents with simple workflows CellROX, fluorescence microscopy/fluorescence imaging, microplate reader, oxidative stress
BioProbes article (2012) Keeping an eye on the messenger fluorescence microscopy/fluorescence imaging, fluorometer, high content analysis nucleic acid quantitation, Qubit
BioProbes article (2012) Ready-to-use reagents shift the focus to results cell structure-nucleus, fluorescence microscopy/fluorescence imaging, high content analysis, sample preparation, viability
BioProbes article (2012) Quantum dots for live-cell imaging—Qdot conjugates go the distance to monitor lipid raft microdomains fluorescence microscopy/fluorescence imaging, lipid rafts, live-cell imaging, particles, Qdot
BioProbes article (2012) New tools for microRNA functional analysis—mirVana miRNA Mimics and Inhibitors fluorescence microscopy/fluorescence imaging, mirVana miRNA mimics, PCR/qPCR, RNAi, TaqMan®
BioProbes article (2012) Automating cell biology—Create your own HCS assay using trusted Molecular Probes dyes antibodies, antibody labeling, cell structure, cell structure-actin, cell structure-lysosomes, cell structure-mitochondria, CellLight, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2012) Unmasking cell replication—Reagents for analyzing cell cycle and replication in mammalian cells cell cycle, cell health cell proliferation, cell structure-nucleus, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
BioProbes article (2013) Beyond Lights Limits: Fluorescence imaging at the nanoscale—Fluorescent probes for three super-resolution modalities antibodies, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent dyes, SIM, STORM, STED, super resolution microscopy
BioProbes article (2013) ReadyProbes ready-to-use imaging reagents for brilliant results apoptosis, cell structure-all, fluorescence microscopy/fluorescence imaging, high content analysis, reagents, Imaging sample preparation, viability
BioProbes article (2013) The EVOS FL Auto Imaging System—Automated imaging. Simplified brightfield microscopy, cell structure-all, EVOS, fluorescence microscopy/fluorescence imaging
BioProbes article (2013) Oxidative stress from a cell's point of view—From CellROX ROS sensors to Image-iT and Click-iT lipid peroxidation detection kits CellROX, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
BioProbes article (2013) Autophagy made visible—Advanced fluorescent probes for imaging distinct processes in the autophagy pathway autophagy, cell structure, cell structure-lysosomes, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2013) Live-cell staining of pluripotent stem cells—Alkaline Phosphatase Live Stain for early identification of induced pluripotent stem cells fluorescence microscopy/fluorescence imaging, live-cell imaging, stem cell research
BioProbes article (2014) Image live cells through time and space—Optimizing conditions for time-lapse fluorescence microscopy cell structure, cell tracking, CellLight, EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging, oxidative stress
BioProbes article (2014) Detect pH inside of a live cell—pHrodo pH sensors for detecting cytosolic and vesicle pH flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader, live-cell imaging, pHrodo
BioProbes article (2014) Obtain the highest-quality data your system can deliver—Fluorescent microspheres for calibrating microscopes and flow cytometers calibration, cell counting, flow cytometer/flow cytometry, flow cytometer set up and calibration, fluorescence microscopy/fluorescence imaging, immunophenotyping, microscope calibration, particles
BioProbes article (2014) Journal Club—An in-depth Look at fluorescent dyes for organelle labeling cell structure-all, fluorescence microscopy/fluorescence imaging, fluorescent dyes
BioProbes article (2014) Introducing a three-channel cell counter for your benchtop—The new and improved Countess II FL Automated Cell Counter cell counting, automated cell counter, brightfield microscopy, Countess, EVOS fluorescence microscopy/fluorescence imaging
BioProbes article (2014) Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometry cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, multicolor flow cytometry
BioProbes article (2015) Applications for therapeutic monoclonal antibodies—Tools for studying antibody internalization and trafficking antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2015) Practical considerations for super-resolution microscopy—Optimize nanoscale imaging at every step fixed-cell imaging ,fluorescence microscopy/fluorescence imaging, SIM, STORM, STED, super resolution microscopy
BioProbes article (2015) ProLong Diamond and SlowFade Diamond Antifade mountants for fixed-cell imaging—Superior protection from photobleaching for fixed cells antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging sample preparation
BioProbes article (2015) ProLong Live Antifade Reagent protects fluorescent proteins and dyes In live-cell imaging—At long last, protection from photobleaching for live cells antifades, fluorescence microscopy/fluorescence imaging, fluorescent proteins, imaging sample preparation, live-cell imaging
BioProbes article (2015) Tools to study mitochondrial morphology and function—Multiplex your mitochondrial data cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, oxidative stress
BioProbes article (2015) Breathe new life into hypoxia research—Intracellular detection of low oxygen tension in live cells antibodies EVOS, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging
BioProbes article (2015) High-content analysis applied to autophagy—Automating cell imaging with Thermo Scientific HCA systems ArrayScan, autophagy, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2015) The EVOS FL Cell Imaging System: A key component of an imaging core facility fluorescence microscopy/fluorescence imaging, EVOS
BioProbes article (2015) Fluorescence-based viability assays for the Countess II FL Automated Cell Counter—Incorporate automation into your cell analysis workflows automated cell counter, cell counting, Countess, EVOS, fluorescence microscopy/fluorescence imaging, viability
BioProbes article (2015) Multiplexable Click-iT Plus TUNEL assays for in situ apoptosis detection—Visualize apoptosis in context apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2015) Formation of uniform and reproducible 3D cancer spheroids in high-throughput plates—Mimic life in three dimensions cancer, fluorescence microscopy/fluorescence imaging, hypoxia, oxidative stress, viability
BioProbes article (2016) A complete suite of reagents, from Cas9 delivery tools to cell function assays—The CRISPR-Cas9 system for genome editing western blotting, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2016) ProLong Live Antifade Reagent is here—Protection from photobleaching for live-cell imaging fluorescence microscopy/fluorescence imaging, Imaging sample preparation, live-cell imaging
BioProbes article (2016) Click-iT EdU and Click-iT TUNEL Colorimetric Assays for immunohistochemistry—Proliferating and apoptotic cells revealed apoptosis, brightfield microscopy, cell proliferation, Click-iT, colorimetric,fixed-cell imaging, immunohistochemistry (IHC)
BioProbes article (2017) Double vision: Simultaneous visualization of protein and RNA targets—ViewRNA Cell Plus Assay for antibody labeling and in situ hybridization in individual cells antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes, in situ hybridization,ViewRNA
BioProbes article (2017) Appetite for destruction—Studying macroautophagy with Premo fluorescent protein–based sensors autophagy, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, immunocytochemistry (ICC), immunofluorescence (IF), live-cell imaging
BioProbes article (2017) Effectively monitor antibody internalization and trafficking—Improved labeling tools for antibody–drug conjugate screening and characterization antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2017) Fueling discovery with high-content imaging and analysis fuel discovery—Thermo Scientific HCA platforms for automated, quantitative analyses high content analysis,  iPSCs, cell viability and function, CellInsight, drug research, neurite outgrowth, neuroscience, quantitative imaging
BioProbes article (2017) Maximize survival of primary cell– and stem cell–derived neurons—Introducing the Gibco B-27 Plus Neuronal Culture System cell culture, fluorescence microscopy/fluorescence imaging, neuroscience, stem cell research
BioProbes article (2017) An evaluation of dyes for tracking cell proliferation by flow and image cytometry cell proliferation, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
BioProbes article (2017) Investigate atherosclerosis with modified low-density lipoproteins—Fluorescently labeled oxidized LDLs and acetylated LDLs atherosclerosis, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent dyes, lipid metabolism, live-cell imaging
BioProbes article (2017) Amplify signals for fluorescence imaging—SuperBoost kits with Alexa Fluor tyramides for signal amplification Alexa Fluor, antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary detection, signal amplification
BioProbes article (2017) Journal club—Coming 'round to spheroid culture spheroids, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, three dimensional cell culture
BioProbes article (2017) Cancer biology in the third dimension—Fluorescent reagents for staining 3D cell cultures created with Cell-Mate 3D matrix cancer, cell culture, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, three dimensional cell culture
Book Live cell imaging Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live cell imaging. Content includes basic history of live cell imaging and case studies of live cell imaging applications Common problems and solutions with live cell imaging.   fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator
eBook Live cell imaging Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live cell imaging. Content includes basic history of live cell imaging and case studies of live cell imaging applications Common problems and solutions with live cell imaging. fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator
Molecular Probes Handbook TSA and other peroxidase-based signal amplification techniques—Section 6.2 Alexa Fluor, antibodies, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), reagents, secondary detection, signal amplification
Molecular Probes Handbook Microspheres—Section 6.5 flow cytometer calibration, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microscope calibration, particles
Molecular Probes Handbook Introduction to signal amplification—Section 6.1 antibodies, fluorescence microscopy/fluorescence imaging, secondary detection, signal amplification, western detection
Molecular Probes Handbook Fluorescence microscopy accessories and reference standards—Section 23.1 fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Using the Fluorescence Spectraviewer—Note 23.1 flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes Handbook Selecting optical filters for fluorescence microscopy—Note 23.2 fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Mitochondria in diseases—Note 12.2 antibodies, cell structure-mitochondria, fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Fluorescent probes for two-photon microscopy—Note 1.5 fluorescence microscopy/fluorescence imaging, two-photon microscopy
Molecular Probes Handbook Fluorescence resonance energy transfer (FRET)—note 1.2 fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Fluorescence polarization (FP)—note 1.4 fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Fluorescence correlation spectroscopy (FCS)—Note 1.3 fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Probes for the endoplasmic reticulum and golgi apparatus—Section 12.4 antibodies, cell structure-er, cell structure-golgi, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes Handbook Probes for mitochondria—Section 12.2 antibodies, cell structure-mitochondria, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes Handbook Probes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3 antibodies, cell structure-lysosomes, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes Handbook Probes for the nucleus—Section 12.5 antibodies, cell structure-nucleus, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability
Molecular Probes Handbook A diverse selection of organelle probes—Section 12.1 cell structure-er, cell structure-golgi, cell structure-lysosomes, cell structure-mitochondria, cell structure-nucleus, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Slow-response probes—Section 22.3 fluorescence microscopy/fluorescence imaging, live-cell imaging, membrane potential dyes
Molecular Probes Handbook Introduction to potentiometric probes—Section 22.1 cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes
Molecular Probes Handbook Fast-response probes—Section 22.2 cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential
Molecular Probes Handbook Other nonpolar and amphiphilic probes—Section 13.5 carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer
Molecular Probes Handbook Introduction to membrane probes—Section 13.1 cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes
Molecular Probes Handbook Fatty acid analogs and phospholipids—Section 13.2 cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent fatty acid analogs, fluorescent lipid dyes, fluorometer
Molecular Probes Handbook Dialkylcarbocyanine and dialkylaminostyryl probes—Section 13.4 carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer
Molecular Probes Handbook Probes for neurotransmitter receptors—Section 16.2 brain hormones, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, receptor binding
Molecular Probes Handbook Probes for following receptor binding and phagocytosis—Section 16.1 cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, internalization, membrane trafficking, phagocytosis, receptor binding, vesicle
Molecular Probes Handbook Probes for tubulin and other cytoskeletal proteins—Section 11.2 cell structure-tubulin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes Handbook Probes for actin—Section 11.1 cell structure-actin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes Handbook Probes useful at near-neutral pH—Section 20.2 BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes Handbook Probes useful at acidic pH—Section 20.3 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook pH indicator conjugates—Section 20.4 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Overview of pH indicators—Section 20.1 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Nucleic acid stains—Section 8.1 cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX
Molecular Probes Handbook Tracers for membrane labeling—Section 14.4 cell structure-plasma membrane, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging
Molecular Probes Handbook Viability and cytotoxicity assay reagents—Section 15.2 cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Assays for apoptosis and autophagy—Section 15.5 apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry
Molecular Probes Handbook Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon
Protocol TO-PRO-3 Stain cell structure, imaging
Protocol SYTOX Green Nucleic Acid Stain cell structure, imaging
Protocol SYTO 82 Nuclear Stain cell structure, imaging
Protocol SYTO 59 Nuclear Stain cell structure, imaging
Protocol ReadyProbes Cell Viability Imaging Kit, Blue/Red cell viability, imaging
Protocol ReadyProbes Cell Viability Imaging Kit, Blue/Green cell viability, imaging
Protocol Pharmacological induction of apoptosis with camptothecin cell vitality, flow cytometry, high content analysis, imaging, microplate
Protocol NucRed Live 647 ReadyProbes Reagent cell structure, imaging
Protocol NucRed Dead 647 ReadyProbes Reagent for viability cell viability, imaging
Protocol NucRed Dead 647 ReadyProbes Reagent for fixed cells cell structure, imaging
Protocol NucGreen Dead 488 ReadyProbes Reagent for viability cell viability, imaging
Protocol NucGreen Dead 488 ReadyProbes Reagent for fixed cells cell structure, imaging
Protocol NucBlue Live ReadyProbes Reagent cell structure, imaging
Protocol NucBlue Fixed Cell ReadyProbes Reagent cell structure, imaging
Protocol LIVE/DEAD Yeast Viability Kit cell viability, imaging
Protocol LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells cell viability, imaging
Protocol LIVE/DEAD Sperm Viability Kit for imaging cell viability, imaging
Protocol LIVE/DEAD Cell Imaging Kit (488/570) cell viability, imaging
Protocol LIVE/DEAD BacLight Bacterial Viability Kit cell viability, imaging
Protocol LIVE BacLight Bacterial Gram Stain Kit cell viability, imaging
Protocol Immunofluorescent staining of intracellular antigens on cultured cells fluorescence microscopy/fluorescence imaging, general ab staining, imaging, immunofluorescence (IF)
Protocol Image-iT DEAD Green Kit cell viability, imaging
Protocol Immunohistochemical staining of formalin-fixed paraffin-embedded tissues general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Indirect method (purified) general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Indirect method (biotin) general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE Tissue Trypsin Digestion—Direct Method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue trypsin digestion antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue low pH antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue low pH antigen retrieval—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue high pH antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue high pH antigen retrieval—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Protocol ICC unfixed cells—Direct method general ab staining, imaging
Protocol ICC methanol fixed cells—Indirect method general ab staining, imaging
Protocol ICC methanol fixed cells—Direct method general ab staining, imaging
Protocol ICC formaldehyde fixed, permeabilized cells—Indirect method general ab staining, imaging
Protocol ICC formaldehyde fixed, permeabilized cells—Direct method general ab staining, imaging
Protocol ICC formaldehyde fixed cells—Indirect method general ab staining, imaging
Protocol ICC formaldehyde fixed cells—Direct method general ab staining, imaging
Protocol Hoechst 33342 for imaging cell structure, imaging
Protocol DAPI for fluorescence imaging cell structure, imaging
Protocol Colorimetric FFPE—trypsin digestion colorimetric staining, general ab staining, imaging
Protocol Colorimetric FFPE—Low pH antigen retrieval colorimetric staining, general ab staining, imaging
Protocol Colorimetric FFPE—High pH antigen retrieval colorimetric staining, general ab staining, imaging
Protocol Click-iT Plus EdU imaging kits cell proliferation, imaging
Protocol Click-iT EdU imaging kits cell proliferation, imaging
Protocol Click-iT EdU HCS assays cell proliferation, imaging
Protocol BrdU labeling and detection cell proliferation, imaging
Protocol ActinRed 555 ReadyProbes Reagent cell structure, imaging
Protocol ActinGreen 488 ReadyProbes Reagent cell structure, imaging
Scientific poster (2006) LipidTOX dyes for adipocyte staining in routine imaging applications  cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability
Scientific poster (2006) Cell cycle analysis using microplate cytometry: A comparison of laser and dye combinations  Acumen Explorer, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2006) New fluorescent reagents for the imaging-based assays for the analysis of drug-induced perturbations of cellular lipid metabolism  ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, microplate reader, viability
Scientific poster (2006) Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer  Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents
Scientific poster (2007) Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry  antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2007) A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry  ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007) Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis  Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2007) Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay  Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling
Scientific poster (2007) BacMam gene delivery of targeted fluorescent proteins, Organelle Lights for HCS  BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, live-cell imaging, nanocrystals, Qdot
Scientific poster (2008) Living molecular probes for the next generation  BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging
Scientific poster (2008) Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization  in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue
Scientific poster (2008) Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents  Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2008) Novel Click-IT TUNEL Assay for detection of cell death  Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability
Scientific poster (2008) Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2008) ThiolTracker Violet dye, a new 405 nm excitable fluorescent probe for detecting intracellular thiols in imaging-based assays  ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, glutathione, high content analysis, microplate reader, protein labeling, violet laser-excited reagents
Scientific poster (2008) Modified tumor cell protein expression and modification under physiological oxygen  Alexa Fluor, cancer, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, physiological oxygen level, protein labeling
Scientific poster (2008) A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays  ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, thiol modification, violet laser-excited reagents
Scientific poster (2009) Cell-based assays for predictive hepatotoxicity measurements using high content imaging  Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability
Scientific poster (2009) A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry  Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection
Scientific poster (2009) Monitoring mitotic cells and DNA content by automated imaging and analysis  Alexa Fluor, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009) Dual pulse labeling using a new thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009) High content imaging of endocytosis & phagocytosis using pHrodo conjugates  Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2009) Click chemistry-based detection of nascent RNA synthesis using high content imaging and fluorescence microscopy  Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, viability
Scientific poster (2009) High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry  Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, viability
Scientific poster (2009) Non-radioactive targeting of multiple classes of protein post-translational modifications (PTMs) with click chemistry  cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling, western blotting
Scientific poster (2009) High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells  cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability
Scientific poster (2009) Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging  Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009) Metabolic labeling and click chemistry detection of stem cell markers  Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein labeling, stem cell research
Scientific poster (2009) Click chemistry-based assays for fluorescence microscopy and high content imaging  Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry  apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010) High content imaging of autophagy  antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader
Scientific poster (2010) Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs  Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment
Scientific poster (2010) Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010) Novel tools to enable high resolution gene expression by investigating the nascent transcriptome  Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, magnetic beads, nucleic acid labeling, PCR/qPCR, viability
Scientific poster (2010) Click chemistry-based enrichment and identification of nascent and post-translationally modified proteins  cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2010) Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis  Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) A new generation of cellular imaging probes for neurosciences research  autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010) ABfinity antibodies: A novel approach to antibody development  Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, immunocytochemistry (ICC), western blotting
Scientific poster (2010) NA-Fluor Influenza Neuraminidase Assay Kit: Standardization of the classic MUNANA-based neuraminidase activity assay for monitoring antiviral resistance in influenza  drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy  apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress
Scientific poster (2010) New fluorescent probes for live-cell imaging  autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis
Scientific poster (2010) The NA-XTD Influenza Neuraminidase Assay Kit: Extended-glow chemiluminescence assay system for highly sensitive influenza neuraminidase assays  chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2010) Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells  cell cycle, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010) Click chemistry enrichment of cell surface glycoproteins  cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2010) Identification and characterization of O-GlcNAc modification of galectin-1 in mesenchymal stem cells using click chemistry  Alexa Fluor, BacMam technology, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, immunocytochemistry (ICC), protein enrichment, protein labeling, western blotting
Scientific poster (2010) BacMam gene expression for functional and imaging applications  BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels
Scientific poster (2010) Click chemistry-based universal enrichment columns for global identification of azide-modified proteins  Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2010) Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010) A universal click chemistry-based enrichment column for the identification of multiple subclasses of PTM-modified proteins and newly synthesized proteins  Alexa Fluor, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2011) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability
Scientific poster (2011) Acoustic cytometric identification and initial characterization of limbal stem cells isolated from human cornea  Attune/Attune NxT, cell culture/expansion, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, PCR/qPCR, stem cell research, tissue, violet laser-excited reagents
Scientific poster (2011) Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2011) Validation of high content imaging and analysis assays for cell health and cytotoxicity  antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection
Scientific poster (2011) The next step in the analysis of lymphocyte proliferation  Alexa Fluor, antibodies, Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, live-cell imaging, violet laser-excited reagents
Scientific poster (2011) The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) NA-Fluor and NA-XTD Influenza Neuraminidase Assays for neuraminidase quantitation and inhibition assays  chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2012) Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy  flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific poster (2013) Cells coming to life: Tools for visualizing immune response  Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection
Scientific poster (2013) Illuminating endocytosis with targeted pH-sensitive fluorescent compounds  Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2014) New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy  autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader
Scientific poster (2015) Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2015) Visualizing the life and death of cells: novel probes and platforms  apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
Scientific poster (2015) Intracellular detection of hypoxia in live cells  EVOS, fluorescence microscopy/fluorescence imaging, hypoxia, EVOS, onstage incubator
Scientific poster (2016) Correlating internalization and potency to accelerate antibody discovery and development brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC)
Scientific poster (2016) A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry  Attune/Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific poster (2016) Incorporating histological analysis into existing high content imaging  antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC)
Scientific poster (2016) Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2017) Matching refractive index of mounting media improves axial resolution and image quality in 3D biological samples  antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent proteins, imaging sample preparation, mountant, onstage incubator, photobleaching
Scientific poster (2017) Hypoxia measurements in live and fixed cells using fluorescence microscopy and high-content imaging  EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, live-cell imaging, onstage incubator
Tutorial

1.2 Fixation–Fixed cell imaging: 5 steps for publication-quality images 
The next step of tissue preparation is fixation. Fixation refers to a chemical means of killing and preserving cells in a particular physiological state, and in many cases, to preserve morphology. Proper fixation equals preservation of target

fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Tutorial

4.1 Mounting media–Fixed cell imaging: 5 steps for publication-quality images 
Using the right mounting media can impact your experiment. Be sure to choose the right type of mountant for your set-up

antifade mounting media, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), mounting media
Tutorial

3.2 Secondary antibody optimization–Fixed cell imaging: 5 steps for publication-quality images 
Secondary antibody detection protocols also need to be optimized for each primary antibody used

antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody protocol, secondary detection
Tutorial

4.2 Photobleaching and antifades–Fixed cell imaging: 5 steps for publication-quality images 
What is photobleaching and how can you prevent it from destroying your sample? Options for antifades are discussed

antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), photobleaching, Prolong Gold, Vectashield
Tutorial

5.5 Ethical considerations–Fixed cell imaging: 5 steps for publication-quality images 
Ethical imaging means trustworthy data, and thus, publishable data. How to treat your samples and data to preserve data integrity is presented

ethical considerations, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF), sample manipulation
Tutorial

5.2 Imaging platforms-software–Fixed cell imaging: 5 steps for publication-quality images 
Taking images on a microscope usually entails having some type of imaging software that aids in taking the image and assists in combining differing colors into one. There are some very important aspects to keep in mind to get a publishable image

FiFI software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, ImageJ, imaging software, imaging systems, immunofluorescence (IF)
Tutorial

1.1 Culture conditions–Fixed cell imaging: 5 steps for publication-quality images 
The first step in obtaining a good image is tissue preparation. For cultured cells, the cells must have good cell health and morphology, as well as good confluency. Healthy cells equal healthy data

EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope
Tutorial

1.5 Autofluorescence–Fixed cell imaging: 5 steps for publication-quality images 
The last step in cell preparation is autofluorescence. Cells and tissue can have a certain degree of autofluorescence that can confuse the specific signal, and lower the signal-to-background. Overcoming autofluorescence means greater sensitivity

autofluorescence, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), tissue autofluorescence
Tutorial

5.4 Image analysis with Celleste software–Fixed cell imaging: 5 steps for publication-quality images 
The functionality of the Celleste softwis reviewed and considerations for processing the image in different software programs are described

Celleste software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF)
Tutorial

1.4 Blocking–Fixed cell imaging: 5 steps for publication-quality images 
The next step after permeabilization is blocking, and there are a number of blocking techniques. Protein blocking equals specific antibody binding. Dye charge blocking means less non-specific binding

dye charge blocking, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), nonspecific binding, protein blocking
Tutorial

2.2 Primary antibody protocol optimization–Fixed cell imaging: 5 steps for publication-quality images 
Every primary antibody must be optimized separately. There are many protocols available, and it is important to understand a "one size fits all" approach gives inferior results, as every antibody is slightly different. Learn how to approach optimization

antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies, primary antibody protocol
Tutorial

5.1 Imaging platforms-hardware–Fixed cell imaging: 5 steps for publication-quality images 
The fifth step of the process is the actual imaging. To capture top-quality images, you need an imaging platform with top-of-the-line imaging capabilities. Here we review considerations for getting the best image

EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope
Tutorial

3.5 Dye choice and special concerns–Fixed cell imaging: 5 steps for publication-quality images 
There are many different dyes spanning the visible, far-red, and infrared wavelengths.Considerations for making the right choices for your experiment are presented

dye choice, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, Fluorescence SpectraViewer, immunofluorescence (IF), label choice, muliparametric dye selection
Tutorial

1.3 Permeabilization–Fixed cell imaging: 5 steps for publication-quality images 
The next step is the permeabilization of the cells which is the key to opening intracellular compartments

cell permeabilization, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Tutorial

3.4 Controls–Fixed cell imaging: 5 steps for publication-quality images 
Researchers should conduct all necessary controls to rule out the possibility of non-specific binding or non-specific signal. Types of controls are described. Proper controls will boost your confidence in your final results

fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging controls, immunofluorescence (IF), sample controls
Tutorial

5.3 Image capture with EVOS FL Auto 2.0–Fixed cell imaging: 5 steps for publication-quality images 
Here the advantages of using the EVOS FL Auto 2.0 imaging system to capture your images are discussed

EVOS FL Auto 2.0, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image capture, immunofluorescence (IF)
Tutorial

3.3 Amplification techniques–Fixed cell imaging: 5 steps for publication-quality images  
If the signal is not strong enough using standard secondary detection schemes, you can increase the signal using amplification techniques. This is particularly important for low-expressing antigens, or rare-cell detection in samples. Examples are discussed

biotin and streptavidin, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), signal amplification, tyramide detection
Tutorial

2.1 Primary antibody choice–Fixed cell imaging: 5 steps for publication-quality images 
After preparation, the second step to publishable images is to label the sample, usually involving primary antibodies to your specific targets of interest. The antibody source, the use of direct versus labeled antibodies as well as the validation for specific applications is discussed

antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies
Tutorial

3.1 Secondary antibody choice–Fixed cell imaging: 5 steps for publication-quality images 
Step three of the five steps in making publishable images is to detect the label. That is, to detect with a secondary antibody, for instance, or an amplification technique, as well as to determine what controls to use. Your options are discussed

antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody, secondary detection
Tutorial

Labeling a purified antibody:Educational video on how to label your next antibody for imaging or fluorescence 
Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.

antibodies, antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF)
Video

Setting up an automated scan routine on the EVOS FL Auto 2 Microscope 
Save time when imaging cells by easily setting up an automated scan routine using the EVOS FL Auto 2 microscope

EVOS, fluorescence microscopy/fluorescence imaging, instrument set-up
Video CellEvent Caspase 3/7 reagent and apoptosis detection   HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal. apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Viability determination of HeLa cells using ReadyProbes Cell Viability Imaging Kit (Blue/Green)   HeLa cells were loaded with NucBlue Live and NucGreen Dead (using 2 drops per ml) in complete media for 15 minutes at 37C. Stauroporine was then added to a final concentration of 1 µM and images were acquired every 30 min. for 18 hours using EVOS Auto Imaging System. All cells are stained with NucBlue Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by the appearance of green nuclei (NucGreen Dead). cell structure-nucleus, cell viability, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Adding oil to objectives on the EVOS FL Auto 2 Microscope 
See how easy it is to add oil to your objective without having to remove your sample when imaging with the EVOS FL Auto 2 Microscope.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video Reviewing Cell Images on the EVOS FL Auto 2 Microscope 
Cell images can be saved from the EVOS FL Auto 2 as a single field, scan, time lapse, and z-stack. Easily review your images and metadata or make adjustments to image brightness, contrast, or gamma and resave your images with the new settings.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video Using the locations tool on the EVOS FL Auto 2 Microscope 
Learn how to create custom locations to use in automated routines for scanning and time lapse experiments on the EVOS FL Auto 2 Cell Imaging System.
EVOS, fluorescence microscopy/fluorescence imaging, instrument set-up
Video How to setup vessel maps on the EVOS FL Auto 2 Microscope 
The EVOS FL Auto 2 can accommodate most vessel types and sizes, including slides, multi-well plates, culture flasks, and petri dishes. See how easy it is to setup the EVOS FL Auto 2 for imaging with the vessel maps tool.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video How to add a scale bar and grid to cell images on the EVOS FL Auto 2 Microscope 
See how easy it is to add a scale bar and grid to your live or captured cell images on the EVOS FL Auto Microscope. You can adjust the grid size, color, and alignment to fit the needs of your experiment.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video Create z-stacks using the EVOS FL Auto 2 Microscope 
Capturing images at different Z-planes can be a powerful tool in fluorescence microscopy. This capability can reveal conditions not seen with standard widefield imaging and easier than ever to use with the EVOS FL Auto 2 microscope.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video LIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System 
Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.
cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging
Video CellTracker Violet reagent and mitosis 
 
U-2 OS cells were transduced with CellLight Tubulin-GFP and Cellular Lights Actin-RFP. The following day cells were labeled with 5uM CellTracker Violet BMQC for 30 minutes at 37C in complete media and washed in fresh media. Images were taken every 5 minutes for 16 hours.
cell structure-all, cell tracking, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Wound healing and cadherin-4 expression 
 
Murine glioma cells were transfected with a DsRed-cadherin-4 fusion, which plays a critical role in cell migration during wound repair, or a GFP control plasmid, then mixed with wild-type cells and seeded in a 24-well plate. After scratch wounding, cell migration was followed over a 21 h period on the EVOS Cell Imaging System equipped with an EVOS Onstage Incubator using a 10X phase objective. Images were captured at an interval of about 10 minutes (116 images total). Video courtesy of Paolo Malatesta, PhD., IRCCS Azienda Ospedaliera Universitaria San Martino -- IST, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
EVOS, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, wound healing
Video Compilation of live cell imaging videos using Invitrogen fluorescent reagents  
This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes.
cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
Video Click-iT Plus TUNEL apoptosis assays  
The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed "click" reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP.
apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging
Video Washing your sample 
Molecular Probes School of Fluorescence explains the wash step common to many cellular imaging protocols.
fluorescence microscopy/fluorescence imaging, imaging sample preparation
Video Z Stack image of HeLa cells labeled with CellLights reagents  
A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights Tubulin-GFP and CellLight Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366 μm.
cell health, cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging
Video How to site specifically label your antibody using SiteClick technology  
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SitClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
Video Mounting a Coverslip 
Molecular Probes School of Fluorescence explains step-by-step how to mount a coverslip properly for cellular imaging. Formulations of mounting media that can add favorable properties such as optimizing the refractive index to match that of glass, preventing photobleaching, or preserving samples for long-term storage are widely available.
fluorescence microscopy/fluorescence imaging, imaging sample preparation
Video Detection of oxidative stress with CellROX Green in U2-OS cells  
U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX Green Reagent (Cat. No. C10444) and 100 nM TMRM in Live Cell Imaging Solution (LCIS, Cat. No. A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core inverted microscope at 37 degrees C. Menadione was added to a final concentration of 100 uM after ten minutes of baseline acquisition. The time lapse data shows the loss of signal from TMRM in the red channel as mitochondrial function decreases, concomitant with the onset of a nuclear signal in the green channel as CellRox Green is oxidized and the reagent migrates to the nucleus to generate a fluorogenic response as the active form of the dye binds to DNA.
CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
Video Time-lapse of cell migration and apoptosis during angiogenesis  
Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator.
angiogenesis, apoptosis, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator
Video Mitochondrial dynamics through cell division  
U2OS cells were transduced with CellLight Mito-RFP and imaged every 5 minutes for 16 hours. Extensive mitochondrial motility is seen throughout mitosis and as the cell regains it's pre mitotic shape following mitosis.
cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Click-iT Plus EdU Proliferation Assay   Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies. cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging
Video Time-lapse with Qtracker 655 cell labeling reagent   Time-lapse image of live cells labeled with Qtracker reagents. Adherent HeLa cells were incubated with Qtracker VIVID 655 Cell Labeling Kit (Cat. No.Q25021MP) at 10 nM for one hour. Images were collected over 90 min using a Zeiss LSM 710 confocal microscope and are shown pseudo-colored magenta. fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Tips and tricks: Phospho Immunofluorescence  cell analysis antibodies, cell imaging, fluorescence microscopy/fluorescence imaging, fixed-cell imaging, immunofluorescence (IF)
Video The secret to solving the most common problem in IF experiments  cell analysis antibodies, cell imaging, fluorescence microscopy/fluorescence imaging, fixed-cell imaging, immunofluorescence (IF)
Video Immunohistochemistry—Tips and tricks for weak or no stain Immunohistochemistry application, offers a spatial visualization and location of an antigen in tissue sections  antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection
Video Immunohistochemistry—Tips and tricks for high background Immunohistochemistry application offers a spatial visualization and location of an antigen in tissue sections. This video offers troubleshooting tips for issues falling into the "high background" category for IHC  antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection
Video Beginner's guide to macro writing: Celleste Image Analysis Software  brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
Video Regions of interest: Celleste Image Analysis Software  brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
Video Cell and object counting: Celleste Image Analysis Software  brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
Video Batch processing: Celleste Image Analysis Software  brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
Webinar Fixed cell imaging—Five steps for publication-quality images 
With over 40 years dedicated to cell imaging research, we offer long-proven tools and protocols to help confidently create quality cell images the first time. This on demand webinar covers the 5 essential steps to getting great images.
antibodies, blocking, Celleste, dyes, EVOS FL Auto 2.0, fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), sample detection, sample labeling, sample preparation, signal amplification
Webinar Learn to choose the right fluorophore when designing experiments 
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore: How to choose the best organic dye for an assay Quantum dots and how they compare to other dyes When to use a phycobiliprotein like R-PE or APC When to use fluorescent proteins like GFP How to choose a suitable dye to match your instrument In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Webinar A practical approach to antibody labeling 
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will: Provide an overview of our antibody labeling kits Offer guidance on which methods are ideal for specific applications and experiments Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar An introduction to immunofluorescence staining of cultured cells 
In this webinar, we discuss the steps of an immunofluorescent staining protocol including material list, common variations, and necessary controls. We'll also provide a simple troubleshooting guide and examine how to avoid common pitfalls. Presented by Jason Kilgore, Technical Support Specialist, Thermo Fisher Scientific.
antibodies, antibody labeling,antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar Basic techniques in autophagy research 
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF)
Webinar The meaning of life at the cellular level: detecting apoptosis with fluorescence 
In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes: An overview of the features of apoptosis Key parameters that can be measured to assay apoptosis A comprehensive guide to available labeling and detection technologies for apoptosis research Tips and tricks to best implement those technologies
apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins
Webinar The meaning of life at the cellular level: Visualizing membrane trafficking 
Membrane trafficking underlies the acquisition and release of cellular material as well as the transport of macromolecules from the site of cellular synthesis to their functional location. Thermo Fisher Scientific offers a number of solutions for researchers using fluorescent probes to study key components of the endocytotic and secretory pathway. This webinar will: Provide an overview of the processes involved in membrane trafficking, along with strategies to investigate them using fluorescence microscopy Offer a comprehensive guide to available labeling and detection technologies for membrane trafficking research Provide tips and tricks on how to best implement those technologies
endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, membrane trafficking, particles
Webinar A comparison of basic immunofluorescent labeling strategies 
In this free webinar, we will compare different immunofluorescent labeling strategies exploring the pros and cons of each method. You will learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We'll also present a simple decision tree to aid in determining the best method for each situation.
Alexa Fluor, antibodies, antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF)
Webinar Breathing new life into hypoxia research 
Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss: Overview of hypoxia in human diseases Classical methods for setting up hypoxic conditions Novel instruments and reagents for imaging cells in hypoxic conditions
EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator
Webinar The meaning of life at the cellular level: Probing viability with fluorescence 
This webinar will provide an overview of features of healthy and unhealthy cells as well as describing key parameters that can be measured to assay cell viability. The webinar also offers a comprehensive guide to available labeling and detection technologies for cell health research as well as tips and tricks on how to best use them.
cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, viability
Webinar Introduction to basic cytoskeleton labeling and detection 
The cytoskeleton is a key component of mammalian cells, providing the framework for cell migration and intracellular transport, furthermore the cytoskeleton regulates cell size and shape as well as important processes such as mitosis and endocytosis. We offer a number of solutions for researchers using fluorescent probes to study the cytoskeleton. This webinar will provide an overview of the structures that comprise the cytoskeleton and important experimental parameters. The webinar also offers a comprehensive guide to available labeling and detection technologies for cytoskeletal research as well as tips and tricks on how to best use them. These tools include those for live-cell imaging fluorescent dyes, antibodies and the BacMam gene delivery platform.
cell structure-actin, cytoskeleton, endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Webinar From the hood to the microscope; Revolutionizing cell-based imaging 
Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. Topics include: Choose a suitable imaging culture vessel for studying cell growth and viability using bright-field microscopy Label cells to maximize signal-to-noise for fluorescence imaging Prepare culture conditions and capture images without losing temporal data for time-lapse imaging Achieve hypoxic culture conditions by modifying gas conditions or employing cell spheroid cultures
cell health, EVOS, FLoid, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator