Zero Blunt® Cloning Kits
The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR®-Blunt vector contains the lethal E. coli ccdB gene fused to the C-terminus of LacZα.
Ligation of a blunt PCR fragment disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed when the transformation mixture is plated.
Speed with Flexibility
| With the addition of the ExpressLink™ T4 DNA Ligase into Zero Blunt® Cloning Kits, ligation can now be performed at room temperature in only 5 minutes with reactions typically yielding >80% recombinants containing inserts. The pCR™-Blunt Vector Features:
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Performance and Value
Zero Blunt® Cloning Kits are available without competent cells (K2750-20 and K2750-40) or with One Shot® TOP10 Chemically Competent E. coli (K2700-20 and K2700-40), in both 20 and 40-reaction sizes.
| Zero Blunt® Cloning Kits | |
|---|---|
| Cloning efficiency | 95% (with control) |
| Time for ligation | 5 minutes |
| Temperature for ligation | Room Temperature |
| PCR Clean-up required? | No |
| Selection Antibiotic | Kanamycin & Zeocin™ |
Kit Contents |
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| Vector |  |
| Ligation Buffer | |
| Forward and reverse sequencing primers | |
| dNTPs | |
| Sterile Water | |
| Control DNA template | |
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.