How are TaqMan Assays kept current as genomic information changes?

TaqMan Assays are systematically re-evaluated using current genomic annotations, variant information, and bioinformatics methods. This evaluation can confirm that an existing assay continues to meet current design criteria, identify an opportunity for redesign, or reveal a new target where portfolio coverage can be expanded.

Do existing TaqMan Assays need to be redesigned when genomic information changes?

Not necessarily. New genomic information can confirm that an existing assay remains an appropriate design or identify an opportunity for improvement. In the recent evaluation of the SNP Genotyping portfolio, the majority of existing assays continued to meet current design criteria.

How are TaqMan Gene Expression Assays updated when transcript annotations change?

TaqMan Gene Expression Assays are evaluated against current gene and transcript annotations to assess coverage and identify gaps or opportunities for improved designs. Using 2026 transcript annotations for human, mouse, and rat, this analysis led to approximately 5700 new Gene Expression Assays and 1300 redesigned assays.

What happens if a known SNP is found within a TaqMan Assay?

Known SNPs are evaluated to determine whether they overlap with a primer or probe binding site and may affect assay performance. If an opportunity for improvement is identified and redesign is technically feasible, the assay may be redesigned. Otherwise, the existing design may be retained if it continues to meet current design criteria.

What was added or redesigned in the TaqMan SNP Genotyping Assay portfolio?

The portfolio evaluation resulted in approximately 660,000 new SNP assay designs and 81,000 redesigned assays.

I have been running a research project and need to continue using a TaqMan Assay that was redesigned or removed as a part of the update; how can I keep ordering the product that I have been using?

Search your order history and find the assay ID. You can order the assay ID through Quick Order.

Lab Tips and Tricks Archives

URL
This field is for validation purposes and should be left unchanged.
First Name
Last Name
Email*
Company
Select your country/region*
Pick a category*
*
- I would like to receive information about content, events, products, services and promotions from Thermo Fisher Scientific and its affiliates. I agree for the Thermo Fisher Scientific group (thermofisher.com/legalentities) to contact me by email and/or telephone to inform me of events, products, services and/or promotions offered in connection with the brands Thermo Scientific™, Applied Biosystems™, Invitrogen™, Gibco™, Ion Torrent ™, Unity Lab Services™, Fisher Scientifc™. I can withdraw my consent and unsubscribe at any time by emailing reply@thermofisher.com By submitting my data, I give consent to the collection, processing and use of my personal data in accordance with the Thermo Fisher Scientific Policy (thermofisher.com/privacypolicy).

What does it take to keep 20 million Applied Biosystems TaqMan A...

AI and Laboratory Automation: The experimental context behind qP...

Building Better Multiplex Panels: Identifying Assay Interactions...

Think your supermix is good enough? Prove it.

Choosing the Right SYBR Green Master Mix for Your Research and B...

Multiplex qPCR primer optimization: How to balance amplification...

When PCR Gets Dirty – Tackling PCR Inhibitors

Multiplex vs. Singleplex qPCR: What do I need to know?

How TaqMan Assays Work

Trust your SYBR Green qPCR Data