Think your supermix is good enough? Prove it.

The chemistry of your qPCR supermix can influence analytical sensitivity, specificity, precision, and overall assay performance. Even when you keep the same instrument, assays, and samples, different master mixes can perform differently.

That’s why we challenge you to put your supermix to the test.

Compare it side by side with an Applied Biosystems Master Mix using your existing workflow.

Take the challenge – Request a sample

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Keep your system. Rethink your supermix.

Already using a Bio-Rad™ CFX Opus™ or other Bio-Rad™ real-time PCR system? Applied Biosystems Master Mixes have demonstrated analytical performance on Bio-Rad™ CFX Opus™ systems, allowing you to compare your current supermix using your existing system and workflow.

To support your comparison, we’ve developed dedicated evaluation guides for some of our most popular qPCR master mixes.

Each evaluation includes:

• Recommended starting cycling conditions for Bio-Rad™ CFX Opus™ systems
• Step-by-step reaction setup
• Side-by-side evaluation scorecards
• Access to technical support, which includes assistance reviewing data from Bio-Rad™ system run files if needed

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Compare qPCR Master Mix Performance

Choose the Applied Biosystems Master Mix that matches your qPCR chemistry, then compare its performance with your current supermix using the same assays, samples, and instrument.

Compare TaqMan Fast Advanced Master Mix to Your Probe-Based Supermix

Low-copy targets can be particularly challenging in multiplex qPCR, where analytical sensitivity, precision, and PCR efficiency can all influence reliable quantification. In comparative testing on a Bio-Rad™ CFX Opus™ Real-Time PCR System, TaqMan Fast Advanced Master Mix was evaluated against Bio-Rad™ SsoAdvanced™ Universal Probes Supermix, Bio-Rad™ iTaq™ Universal Probes Supermix, and Bio-Rad™ iQ™ Multiplex Powermix. Under the study conditions, TaqMan Fast Advanced Master Mix demonstrated greater or equal precision across the evaluated multiplex targets compared with the Bio-Rad supermixes tested. At 0.0256 ng cDNA per reaction, replicate amplification profiles were evaluated across FAM, VIC, and JUN targets. Across the dilution series, TaqMan Fast Advanced Master Mix demonstrated PCR efficiencies of 101%, 102%, and 97% for FAM, VIC, and JUN, respectively.

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Compare Your SYBR Green Supermix to PowerUp or PowerTrack SYBR Green Master Mix

With SYBR Green chemistry, every fluorescent signal matters—but not every signal is from your target. Nonspecific amplification and primer-dimers can contribute to fluorescence, making analytical specificity an important consideration when choosing a SYBR Green master mix.

Compare your current SYBR Green supermix with PowerUp or PowerTrack SYBR Green Master Mix and evaluate performance using your own assays, samples, and qPCR system.

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Compare SYBR Green assay specificity across master mixes

Melt curve analysis can help assess SYBR Green assay specificity. A single melt peak suggests amplification of a single product, while additional peaks can indicate nonspecific amplification or primer-dimer formation.

In comparative testing on a Bio-Rad™ CFX Opus™ Real-Time PCR System, melt curve profiles were evaluated across three representative SYBR Green assays using PowerTrack and PowerUp SYBR Green Master Mixes and two Bio-Rad supermixes.

Which Applied Biosystems SYBR Green Master Mix Should You Choose?

Both PowerTrack and PowerUp SYBR Green Master Mixes include premixed ROX dye and UDG. The key differences are the performance and workflow features that may matter for your application.

	PowerTrack SYBR Green Master Mix	PowerUp SYBR Green Master Mix
Recommended for	High analytical sensitivity & reaction setup QC	Highest analytical specificity
Tracking dye	Yes	No
Benchtop stability	8 hours	72 hours
DNA polymerase	Taq with antibody-mediated hot start	Taq with dual lock double hot start
ROX dye	Included, premixed	Included, premixed
UDG	Included	Included

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Challenge accepted?

The best way to evaluate a master mix isn’t by reading about it. It’s by running it.

Request an evaluation sample, download the guide that matches your workflow, and compare an Applied Biosystems Master Mix with your current supermix. Then decide based on what your data tell you.

Request a Sample*

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Frequently Asked Questions

For Research Use Only. Not for use in diagnostic procedures. © 2026 Thermo Fisher Scientific Inc. All rights reserved. Bio-Rad, CFX Opus, SsoAdvanced, iTaq, and iQ are trademarks of Bio-Rad Laboratories, Inc. TaqMan is a trademark of Roche Molecular Systems, Inc., used under permission and license. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

Terms and conditions: No purchase necessary. This promotion is available to life science professionals 21 years or older. Approx. retail value of sample is $125–$525 USD depending on the sample selected. Promotion runs until sample supplies are depleted. The sample volume is 1 mL. Limit one free sample per customer. By accepting the sample, you warrant that you are not prohibited by employment, contract, or law from accepting a sample from Life Technologies and that your employer has full knowledge of your actions and has consented to you receiving a free sample from Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.