Hoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Color
Blue
Excitation Wavelength Range
352 nm
Dye Type
Hoechst 33258
For Use With (Application)
Fluorescent labeling
For Use With (Equipment)
Fluorescence Microscope
Quantity
10 mL
Detection Method
Fluorescence
Description
Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg/mL Solution in Water
Emission
461 nm
Form
Liquid
Product Line
Hoechst
Shipping Condition
Room Temperature
Product Type
Nucleic Acid Stain
SubCellular Localization
Nucleus, Nucleic Acids
Contents & Storage
Store in refrigerator at 2°C to 8°C and protect from light.