Applied Biosystems™

GeneChip™ Wash Buffer A

GeneChip™ Wash Buffer A is a component of the GeneChip Hybridization, Wash, and Stain Kit, but may be purchased separately.

Katalognummer	Menge
900721	800 mL

Katalognummer 900721

Preis (EUR)

Menge:

800 mL

GeneChip™ Wash Buffer A is a component of the GeneChip Hybridization, Wash, and Stain Kit, but may be purchased separately. The GeneChip Hybridization, Wash, and Stain Kit is used with GeneChip brand arrays in cartridge format, following the target preparation steps described for 3' eukaryotic, prokaryotic, and exon assays.

Functionally tested on GeneChip Brand Arrays, we recommend the Prokaryotic Target Labeling Assay and associated reagents for use in prokaryotic gene expression studies.

Prokaryotic Target Labeling Assay components include:

Control Oligo B2, 3nM
GeneChip DNA Labeling Reagent
GeneChip Poly-A RNA Control Kit

Related Links:

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Zur Verwendung mit (Anwendung)Transcriptome Profiling

ProduktlinieGeneChip

Menge800 mL

ReagenztypWash Buffer

TypWash Buffer A

Unit SizeEach

Häufig gestellte Fragen (FAQ)

Can I use the GeneChip Wash Buffer A in a Fluidics station even if I can see crystals/precipitate present in the buffer?

GeneChip Wash Buffer A can be kept at room temperature (overnight) the day before the experiment. If you see crystals, we recommend equilibrating the buffer up to 4 hrs (if no external heating is used) until it reaches room temperature. You can also vortex it with a stir bar at room temperature.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the shelf life of this the DNA Labeling reagent (Cat. No. 900542)?

The DNA Labeling Reagent has about 12 months of dating from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Do the reagents in the GeneChip HT Hybridization, Wash, and Stain Kit (Cat. No. 901219) have the same formulation as the GeneChip Hybridization, Wash, and Stain Kit for cartridges (Cat. No. 900720)?

The GeneChip HT hybridization, Wash Stain Kit (Cat. No. 901219) is part of an older kit that is discontinued and no longer available.
The kit consisted of:
- HT Pre-Hyb mix, HT 1.2X Hyb mix, HT Stain Cocktail 1& 3, HT Stain Cocktail 2, HT Wash Buffers A, HT Wash Buffer B, and HT Array Holding Buffer.

The GeneChip HWS kit (Cat. No. 900720) is a current product that can be ordered.
This kit consists of:
- Pre-Hyb module, 2X Hyb mix, DMSO, Nuclease Free Water, Stain Module (Stain Cocktail 1 and Stain Cocktail 2) Wash Buffers, Wash Buffer B, and Array Holding Buffer.

The HT kit was formulated for high throughput usage. Therefore, the formulations differ. The Wash buffers A and B are similar, but they have slight variations in terms of additives.
The Hyb mixes have different concentrations. The stain cocktails have different stain mixtures (Stain 1 & Stain 3 vs Stain 1).
Therefore, we do not recommend swapping any of these reagents.
Only the Array Holding Buffer is the same and could be swapped.
The exact components and concentrations of the reagents are proprietary information and thus cannot be disclosed.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.