FastDigest NotI
FastDigest NotI
Thermo Scientific™

FastDigest NotI

5' G C ↓G G C C G C 3' 3' C G C C G G ↑C G 5'Read more
Catalog NumberQuantity
FD0595150 μL (150 Reactions)
FD059320 μL (20 Reactions)
FD0596250 μL (250 Reactions)
FD059450 μL (50 Reactions)
4 Options
Catalog number FD0595
Price (JPY)/ 150 reactions
Price:52,700
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Quantity:
150 μL (150 Reactions)
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5'  G  C ↓G  G  C  C  G  C   3' 
3'  C  G  C  C  G  G ↑C  G   5' 

Thermo Scientific FastDigest NotI restriction enzyme recognizes GC^GGCCGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: CciNI.

Thermo Scientific FastDigest NotI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Methylation SensitivityCpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
EnzymeNot I
Compatible Buffer10X FastDigest Buffer/FastDigest Green Buffer
Sensitive to Heat InactivationYes
Optimal Reaction Temperature37°C
Type IIS RENo
Quantity150 μL (150 Reactions)
Product TypeRestriction Enzyme
Research CategoryTraditional Cloning
Product LineFastDigest™
Overhang5' protruding ends
Inactivation Temperature80°C
Unit Size150 reactions
Contents & Storage
• FastDigest NotI (150 μL)
• 10X FastDigest Buffer (1.0 mL)
• 10X FastDigest Green Buffer (1.0 mL)

Store at –20°C.