Lipofectamine Reagent | Thermo Fisher Scientific

Since launching in 1993, Invitrogen Lipofectamine reagents have become the most referenced transfection reagents with over 50,000 citations to date. You continue to choose these reagents due to their wide acceptance as the best and most reliable available. Lipofectamine reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use.

Our Lipofectamine reagent–specific protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types (see "Download protocol" links in the table below). This is often the best place to start especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can you look to our cell-specific protocols for further optimization.

Which Invitrogen Lipofectamine product is right for you?

	High efficiency versatile reagent for a wide range of common cell types	Most efficient versatile reagent for the widest range of cell types including difficult-to-transfect cells	Most efficient reagent for siRNA/miRNA delivery, efficient gene knock-down	Most efficient reagent for mRNA delivery. Best for neurons and primary cell types	Superior transfection efficiency in even the most difficult stem cells
Name	Lipofectamine 2000 Transfection Reagent	Lipofectamine 3000 Transfection Reagent	Lipofectamine RNAiMAX Transfection Reagent	Lipofectamine MessengerMAX Transfection Reagent	Lipofectamine Stem Reagent
Top-selling format	Cat. No. 11668-019 (1.5 mL)	Cat. No. L3000-015 (1.5 mL)	Cat. No. 13778-075 (0.75 mL)	Cat. No. LMRNA008 (0.75 mL)	Cat No. STEM00008 (0.75 mL)
Sample type	DNA RNA Co-transfection	DNA RNA Co-transfection	RNA	mRNA	DNA mRNA siRNA Cas 9 RNP Co-transfection
Transfection efficiency	High	Superior	Superior	Superior	Superior
Cell viability	High	Superior	Superior	Superior	Superior
Protocol	Download protocol	Download protocol	Download protocol	Download protocol	Download protocol
	Order now	Order now	Order now	Order now	Order now

See our Neon Transfection System for electroporation

See our CRISPR Transfection Selection guide

Meet the inventor

R&D transfection insights
Xavier de Mollerat du Jeu, Sr. Staff Scientist, discusses the transfection research program and key factors for successful transfections, which resulted in enhanced protocols for the Lipofectamine line of transfection reagents.