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Since launching in 1993, Invitrogen Lipofectamine reagents have become the most referenced  transfection reagents with over 50,000 citations to date. You continue to choose these reagents due to their wide acceptance as the best and most reliable available. Lipofectamine reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use.

Our Lipofectamine reagent–specific protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types (see "Download protocol" links in the table below). This is often the best place to start especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can you look to our cell-specific protocols for further optimization.

Which Invitrogen Lipofectamine product is right for you?



Product
Lipofectamine 3000
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Lipofectamine 2000
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Lipofectamine RNAiMAX
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Lipofectamine MessengerMAX
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Lipofectamine CRISPRMAX
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Lipofectamine Stem
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Cell Type
Workhorse Workhorse Workhorse Workhorse Workhorse  
Hard-to-transfect   Hard-to-transfect   Hard-to-transfect  
Primary   Primary Primary Primary  
Stem Cells   Stem Cells Stem Cells   Stem Cells
  Neurons   Neurons    
Cancer Cancer Cancer Cancer Cancer  




Payload
DNA DNA       DNA
    siRNA     siRNA
      mRNA   mRNA
    miRNA      
        CRISPR-Cas9 RNP CRISPR-Cas9 RNP
Cotransfection Cotransfection        
  in vitro delivery
Protocol Download protocol Download protocol Download protocol Download protocol Download protocol Download protocol

Meet the inventor

R&D transfection insights
Xavier de Mollerat du Jeu, Sr. Staff Scientist, discusses the transfection research program and key factors for successful transfections, which resulted in enhanced protocols for the Lipofectamine line of transfection reagents.

leaf icon Sustainable packaging

Packaging must protect the product inside—but that doesn’t have to mean sacrificing sustainability. Lipofectamine products are shipped at ambient temperature, diverting 24,000 ft3 of Expanded Polystyrene (EPS) coolers from landfills annually. We reduce the carbon footprint from packaging and shipping these products by 80 tons (CO2 equivalents).

Learn more about sustainable solutions
Download the green fact sheet