Since launching in 1993, Invitrogen Lipofectamine reagents have become the most referenced transfection reagents with over 50,000 citations to date. You continue to choose these reagents due to their wide acceptance as the best and most reliable available. Lipofectamine reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use.
Our Lipofectamine reagent–specific protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types (see "Download protocol" links in the table below). This is often the best place to start especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can you look to our cell-specific protocols for further optimization.
NEW Invitrogen TransfectionSelect™ product selection tool
Which Invitrogen Lipofectamine product is right for you?
|Lipofectamine 3000||Lipofectamine 2000||Lipofectamine RNAiMAX||Lipofectamine MessengerMAX||Lipofectamine CRISPRMAX||Lipofectamine Stem|
|Stem Cells||Stem Cells||Stem Cells||Stem Cells|
|CRISPR-Cas9 RNP||CRISPR-Cas9 RNP|
|in vitro delivery|
|Protocol||Download protocol||Download protocol||Download protocol||Download protocol||Download protocol||Download protocol|
Meet the inventor
R&D transfection insights
Xavier de Mollerat du Jeu, Sr. Staff Scientist, discusses the transfection research program and key factors for successful transfections, which resulted in enhanced protocols for the Lipofectamine line of transfection reagents.
Packaging must protect the product inside—but that doesn’t have to mean sacrificing sustainability. Lipofectamine products are shipped at ambient temperature, diverting 24,000 ft3 of Expanded Polystyrene (EPS) coolers from landfills annually. We reduce the carbon footprint from packaging and shipping these products by 80 tons (CO2 equivalents).
For Research Use Only. Not for use in diagnostic procedures.