Consider upgrading to TrueCut Cas9 Protein v2 for higher editing efficiency.
Invitrogen™

Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent

Catalog number:  CMAX00003

 Related applications:

Primary Cell Culture

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Description

Lipofectamine CRISPRMAX Cas9 Transfection Reagent is the first optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery. It is a high-throughput-friendly, cost-effective alternative to electroporation.

Working together with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA, it provides:
• Demonstrated cleavage efficiency—tested in over 20 cell types including iPSC, mESC, N2A, CHO, A549, HCT116, HeLa, HEK293, and several others
• Low cell toxicity—less cells needed to initiate your experiment
• High throughput friendly—an ideal delivery solution for 96-well format
• Cost savings—whether cost-per-reaction or initial investment

Achieve high transfection efficiency in novel genome editing applications
Lipofectamine CRISPRMAX transfection reagent increases the likelihood of successful cleavage and recombination, especially when combined with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA. This ultimately maximizes the efficiency of performing genetic modifications.

Improve experimental outcomes using TrueCut Cas9 Protein v2
Unlike CRISPR plasmid or Cas9 mRNA, using Cas9 protein provides superior cleavage efficiency in primary cells and stem cells. It eliminates the need for transcription or translation of the payload, removes the risk of genomic integration, and is cell cycle independent.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Primary Cells
Sample Type (Specific): Protein
Classification: Animal Origin-Free
Transfection Technique: Lipid-Based Transfection
Serum Compatible: Yes
Product Line: CRISPRMAX™, Lipofectamine®
Product Size: 0.3 mL
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Contains one vial of Lipofectamine CRISPRMAX Reagent and one vial of Cas9 Plus Reagent.

Store at 2–8°C. Do not freeze.

Documents

Manuals & protocols