TO-PRO®-3 Stain

TO-PRO®-3 stain is an excellent far red-fluorescent nuclear and chromosome counterstain that is impermeant to live cells but penetrates compromised membranes characteristic of dead cells, making it a useful indicator of dead cells within a population. The carbocyanine-based dye exhibits far-red fluorescence with excitation at 642 nm and emission at 661 nm. The long-wavelength fluorescence of TO-PRO®-3 stain is well separated from that of commonly used fluorophores imaged in the DAPI and FITC channels.

Long-wavelength light–absorbing dyes such as TO-PRO®-3 stain have the advantage that their fluorescence is usually not obscured by tissue autofluorescence. TO-PRO®-3 stain has very strong binding affinity for dsDNA, with dissociation constants in the micromolar range. It gives strong and selective staining of the nucleus in cultured cells and in paraffin sections. 

TO-PRO®-3 stain is among the most sensitive probes for nucleic acid detection, and in addition to cell staining it can be used for staining nucleic acids on solid supports and pre-staining samples for gel or capillary electrophoresis.

TO-PRO®-3 stain dashboard


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TO-PRO®-3 stain is a nucleic acid stain with far-red fluorescence that is a very sensitive nuclear counterstain and dead cell indicator.

 
   

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  633 or 647 Cy®5 642 661  
 
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TO-PRO®-3 stain products

We offer TO-PRO®-3 stain as an iodine salt in DMSO. TO-PRO®-3 stain is also included in the SelectFX® Nuclear Labeling Kit, which provides four spectrally distinct fluorescent dyes for staining nuclei in fixed-cell preparations.

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Fluorescence immunostaining of a fixed co-culture of murine neurons and dendritic cells. Fluorescence immunostaining of a fixed co-culture of murine neurons and dendritic cells. Neurons were labeled with an antibody directed against neurofilament triplet H (NFH) protein and visualized using red-fluorescent tetramethylrhodamine goat anti–mouse IgG.

Dendritic cells were labeled with a biotin conjugate of a primary antibody directed against the integrin CD11c and visualized using green-fluorescent Alexa Fluor® 488 streptavidin (Cat. no. S11223, S32354). Nuclei were stained with blue-fluorescent TO-PRO®-3. Image submitted by Gauthier Dorban, Institute of Human Histology, University of Liège, Belgium.

High-performance nucleic acid stains

We offer a range of nuclear counterstains to provide highly selective nuclear staining with little or no cytoplasmic labeling and a choice of colors for multiplexing with other labels. Additionally, some of the stains can be used in cell-cycle analyses with high-content imaging and flow cytometry.

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FluoCells prepared slide #6 showing a fixed, permeabilized, and labeled muntjac skin fibroblast.

Mitochondria were labeled with mouse anti–OxPhos Complex V inhibitor protein antibody and visualized using orange-fluorescent Alexa Fluor® 555 goat anti–mouse IgG antibody. F-actin was labeled with green-fluorescent Alexa Fluor® 488 phalloidin, and the nucleus was stained with TO-PRO®-3 iodide (pseudocolored magenta).

Resources

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with TO-PRO®-3 stain and a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.