Invitrogen™

Streptavidin, Alexa Fluor™ 488 Conjugate

Catalog number: S32354
Invitrogen™

Streptavidin, Alexa Fluor™ 488 Conjugate

Catalog number: S32354
Catalog Number
S32354
also known as S-32354
Unit Size
0.5 mL
Price (USD)
318.00
Your Price:
Availability
***
Quantity
Catalog Number
S11223
also known as S-11223
Unit Size
1 mg
Price (USD)
273.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
S32354
also known as S-32354
0.5 mL
318.00
Your Price:
***
S11223
also known as S-11223
1 mg
273.00
Your Price:
***
Product Overview
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Additional Information
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Alexa Fluor™ 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 488 Streptavidin Conjugates:
Alexa Fluor™ 488 streptavidin conjugate has Ex/Em maxima of ∼ (495/519)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor™ Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Specifications

Concentration
2 mg/mL
Conjugate
Alexa Fluor 488
Quantity
0.5 mL
Shipping Condition
Wet Ice
Form
Liquid
Product Line
Alexa Fluor™
Product Type
Streptavidin Conjugate (fluorescent)

Contents & Storage

Store in refrigerator (2–8°C) and protect from light.

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