3D Cell Culture Protocols and Organoid Culture Methods

There is a diverse range of protocols available in the literature around 3D cell culture and analysis. Discover those highlighted here to learn how researchers use Invitrogen and Gibco products in their methods.

Overview of the cell lines, culture conditions, and analysis techniques that are being used in the transition from 2D to 3D cell models in the 3D Cell Culture & Analysis Handbook.

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To help guide you in your 3D cell culture, our R&D teams have developed suggested protocols for the generation of spheroids from the most commonly used cancer cell lines using Nunclon Sphera cell culture plastics and Gibco media.

• LNCaP Cell Line Spheroid Generation and Characterization for HT Assays
• MDA-MB-231 Cell Line Spheroid Generation and Characterization for HT Assays
• PANC-1 Cell Line Spheroid Generation and Characterization for HT Assays
• PC-3 Cell Line Spheroid Generation and Characterization for HT Assays
• SKOV-3 Cell Line Spheroid Generation and Characterization for HT Assays
• T47D Cell Line Spheroid Generation and Characterization for HT Assays
• HepG2 Cell Line Spheroid Generation and Characterization for HT Assays
• SW480 Cell Line Spheroid Generation and Characterization for HT Assays

• AlgiMatrix 3D Culture System
  
• Growing Cells in Geltrex Reduced Growth Factor Basement Membrane Matrix
• In Situ Harvesting of Spheriods from AlgiMatrix Plates
• Isolation of Viable Spheroids from AlgiMatrix
• Quantifying Viability of Isolated Spheroids from AlgiMatrix Using CyQUANT Proliferation Kit
• Using AlgiMatrix Firming Buffer with 24-well and 96-well AlgiMatrix 3D Culture System Plates

Cancer organoids are known to have complex culture requirements that can be challenging. The OncoPro Tumoroid Culture Medium Kit helps simplify tumoroid culture with well-defined, easy-to-follow protocols, helping make physiologically relevant cancer models more accessible.

• Glioblastoma and Gliosarcoma Organoid Protocols