Informational:Informational:Save up to 31% on your order of cell culture products. View promotion details »
Gibco™

Advanced DMEM/F-12

Catalog number: 12634010
Gibco™

Advanced DMEM/F-12

Catalog number: 12634010
Catalog Number
12634010
Unit Size
500 mL
Price (USD)
Price: 68.50
Online price: 63.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
10 x 500 mL
Price (USD)
Price: 588.00
Online price: 522.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12634010500 mL
Price: 68.50
Online price: 63.65
Your price:
-
1263402810 x 500 mL
Price: 588.00
Online price: 522.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

This Advanced DMEM/F-12 is manufactured as follows:
WithWithout
• Glucose• L-glutamine
• Non Essential Amino Acids• HEPES
• Sodium pyruvate 
• Phenol Red 


The complete formulation is available.

Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line
MRC-5, SP2, Vero, WI-38 and Jurkat
Classification
Animal Origin
Concentration
1 X
Form
Liquid
Product Type
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Serum Level
Reduced Serum
Shelf Life
12 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without Additives
No Glutamine, No HEPES
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Quantity
500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text