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Citations & References (7)
Thermo Scientific™
RIPA Lysis and Extraction Buffer
Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysisRead more
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| Catalog Number | Quantity |
|---|---|
| 89900 | 100 mL |
| 89901 | 250 mL |
Catalog number 89900
Price (JPY)Contact Us ›
27,500
Each
Quantity:
100 mL
Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
Features of RIPA Buffer:
• Convenient—ready-to-use solution; no need to assemble and prepared components yourself
• Flexible—compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
• Versatile—enables extraction of cytoplasmic, membrane and nuclear proteins
• Disclosed formulation—contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues
This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.
RIPA buffer derives its name from the original application for which it was developed: the radio-immunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.
Related Products
Pierce™ IP Lysis Buffer
M-PER™ Mammalian Protein Extraction Reagent
Features of RIPA Buffer:
• Convenient—ready-to-use solution; no need to assemble and prepared components yourself
• Flexible—compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
• Versatile—enables extraction of cytoplasmic, membrane and nuclear proteins
• Disclosed formulation—contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues
This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.
RIPA buffer derives its name from the original application for which it was developed: the radio-immunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.
Related Products
Pierce™ IP Lysis Buffer
M-PER™ Mammalian Protein Extraction Reagent
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatLiquid
Quantity100 mL
Volume (Metric)100 mL
Product TypeExtraction Buffer
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.
Frequently asked questions (FAQs)
What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?
Does RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) contain protease or phosphatase inhibitors?
Why is it not recommended that I use RIPA buffer for protein A280 measurements with my NanoDrop spectrophotometer?
Why is there low phosphorylation of the proteins when I use the RIPA Lysis and Extraction Buffer?
What if proteolysis occurs when I use RIPA Lysis and Extraction Buffer?
Citations & References (7)
Citations & References
Abstract
Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation.
Journal:European journal of nutrition
PubMed ID:38214710
PURPOSE: Shatavari is an understudied, widely available herbal supplement. It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari's actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples
Cerebral microvascular endothelial cell-derived extracellular vesicles regulate blood - brain barrier function.
Journal:Fluids and barriers of the CNS
PubMed ID:38114994
Autoreactive T lymphocytes crossing the blood-brain barrier (BBB) into the central nervous system (CNS) play a crucial role in the initiation of demyelination and neurodegeneration in multiple sclerosis (MS). Recently, extracellular vesicles (EV) secreted by BBB endothelial cells (BBB-EC) have emerged as a unique form of cell-to-cell communication that contributes
Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure.
Journal:Proteomes
PubMed ID:36136308
The comprehension of the pathophysiological mechanisms, the identification of druggable targets, and putative biomarkers for aortic valve stenosis can be pursued through holistic approaches such as proteomics. However, tissue homogenization and protein extraction are made difficult by tissue calcification. The reproducibility of proteome studies is key in clinical translation of
TGFB1-induced extracellular expression of TGFBIp and inhibition of TGFBIp expression by RNA interference in a human corneal epithelial cell line.
Journal:Invest Ophthalmol Vis Sci
PubMed ID:20881301
'To report the increased production of extracellular transforming growth factor ß-induced protein (TGFBIp) by human corneal epithelial cells (HCECs) after induction by TGFB1 and the inhibition of TGFBIp production in induced and noninduced HCECs by RNA interference (RNAi).'
Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs.
Journal:Osteoarthritis Cartilage
PubMed ID:19128988
Synovium-derived stem cells (SDSCs) have proven to be superior in cartilage regeneration compared with other sources of mesenchymal stem cells. We hypothesized that conventionally passaged SDSCs can be engineered in vitro into cartilage tissue constructs and the engineered premature tissue can be implanted to repair allogeneic full-thickness femoral condyle cartilage