Key industry publications and methods
There is a diverse range of protocols available in the literature around 3D cell culture and analysis. Discover those highlighted here to learn how researchers use Invitrogen and Gibco products in their methods.
FREE 3D eLearning Courses
We are now offering an ever-expanding set of free e-Learns to pass on the expertise and assist with advancing your research. Follow the links below and start your free learning adventure in the basics of 3D and then progress to our neural organoid training course.
Getting started in 3D cell culture
Learn more at: thermofisher.com/3dbasics-elearn
Neural organoid generation from PSCs
Learn more at: thermofisher.com/neuralorganoid-elearn
Brain
Liver
Intestine
Kidney
Lung
Cell type | Relevant growth factors | Relevant media | Key publication |
---|---|---|---|
Pluripotent stem cells | Activin, FGF4, FGF2, Noggin, SHH | RPMI1640, Advanced DMEM/F12 | Dye, BR., et al., Elife. 2015 |
Pluripotent stem cells | FGF2 | Advanced DMEM/F12 | Wilkinson, DC., et al., Stem Cells Translational Medicine. 2016 |
Brain
Liver
Intestine
Kidney
Lung
Cell type | Relevant growth factors | Relevant media | Key publication |
---|---|---|---|
Pluripotent stem cells | Activin, FGF4, FGF2, Noggin, SHH | RPMI1640, Advanced DMEM/F12 | Dye, BR., et al., Elife. 2015 |
Pluripotent stem cells | FGF2 | Advanced DMEM/F12 | Wilkinson, DC., et al., Stem Cells Translational Medicine. 2016 |
Protocols for spheroid generation
To help guide you in your 3D cell culture, our R&D teams have developed suggested protocols for the generation of spheroids from the most commonly used cancer cell lines using Nunclon Sphera cell culture plastics and Gibco media.
- LNCaP Cell Line Spheroid Generation and Characterization for HT Assays
- MDA-MB-231 Cell Line Spheroid Generation and Characterization for HT Assays
- PANC-1 Cell Line Spheroid Generation and Characterization for HT Assays
- PC-3 Cell Line Spheroid Generation and Characterization for HT Assays
- SKOV-3 Cell Line Spheroid Generationand Characterization for HT Assays
- T47D Cell Line Spheroid Generationand Characterization for HT Assays
- HepG2 Cell Line Spheroid Generation and Characterization for HT Assays
- SW480 Cell Line Spheroid Generation and Characterization for HT Assays
Protocols for matrix-based 3D cell culture
- AlgiMatrix 3D Culture System
- Growing Cells in Geltrex Reduced Growth Factor Basement Membrane Matrix
- In Situ Harvesting of Spheriods from AlgiMatrix Plates
- Isolation of Viable Spheroids from AlgiMatrix
- Quantifying Viability of Isolated Spheroids from AlgiMatrix Using CyQUANT Proliferation Kit
- Using AlgiMatrix Firming Buffer with 24-well and 96-well AlgiMatrix 3D Culture System Plates
For Research Use Only. Not for use in diagnostic procedures.