Key industry publications and methods

There is a diverse range of protocols available in the literature around 3D cell culture and analysis. Discover those highlighted here to learn how researchers use Invitrogen and Gibco products in their methods.

Brain

Brain

Cell type Relevant growth factors Relevant media Key publication
Pluripotent stem cells FGF2 DMEM/F12, Neurobasal Lancaster, M.A., et al. Nature. 2013 
Pluripotent stem cells FGF2, EGF, BDNF, NT-3 DMEM/F12, Neurobasal Pasca, A.M. et al. Nature Methods. 2015 
Pluripotent stem cells DKK1 DMEM/F12 Phillips, A.W., et al. J. Vis. Exp. 2017 
Pluripotent stem cells DKK1, BMPR1a-Fc DMEM/F12, Neurobasal Mariani, J., et al. PNAS. 2012 
Pluripotent stem cells N/A DMEM/F12, Neurobasal Iefremova, V., et al., Cell Reports. 2017 
Pluripotent stem cells BDNF, GDNF, NT-3, Laminin, bFGF DMEM/F12, Essential 8, Essential 6, Neurobasal Zhou, T., et al., Cell Stem Cell. 2017 
Liver

Liver

Cell type Relevant growth factors Relevant media Key publication
Adult tissue stem/progenitor cells FGF10, HGF, EGF, Noggin Advanced DMEM/F12 Huch, M., et al. Nature. 2013 
Adult tissue stem/progenitor cells EGF, FGF10, HGF, Noggin, BMP7, FGF19 Advanced DMEM/F12 Huch, M., et al. Cell 2015 
Pluripotent stem cells BMP4, VEGF, Activin, PDGFBB, FGF2 RPMI1640, DMEM/F12, StemPro-34 SFM Takebe, T., et al. Cell Reports. 2017 
Pluripotent stem cells FGF2, BMP4, Activin, HGF, EGF RPMI1640 Takebe, T., et al. Nature. 2013 
Primary cells N/A HepaRG media Proctor, W.R., et al. Arch. Toxicol. 2017 
Intestine

Intestine

Cell type Relevant growth factors Relevant media Key publication
Adult tissue stem/progenitor cells Noggin, EGF, FGF10 DMEM, Advanced DMEM/F12 Sato, T, et al., Gastroenterology. 2011 
Adult tissue stem/progenitor cells EGF, Noggin Advanced DMEM/F12 Sato, T., et al., Nature, 2009 
Pluripotent stem cells Activin, FGF4, BMP2, EGF, Noggin, SHH N/A Munera, J.O., et al., Cell Stem Cell. 2017 
Pluripotent stem cells Activin, FGF4, Noggin, EGF DMEM/F12, RPMI1640, Advanced DMEM/F12 Spence, J.R., et al., Nature. 2011 
Pluripotent stem cells IGF1, FGF2 Knockout DMEM Uchida, H., et al., JCI Insight. 2016 
Pluripotent stem cells Activin, FGF4, EGF, Noggin RPMI1640, Advanced DMEM/F12 Watson, C.L., et al., Nature Medicine. 2014 
Kidney

Kidney

Cell type Relevant growth factors Relevant media Key publication
Pluripotent stem cells
 FGF2, Noggin, Activin, FGF9 Advanced RPMI1640 Morizane, R., et al., Nature Biotechnology. 2015 
Pluripotent stem cells
 FGF2, Noggin, Activin, FGF9 Advanced RPMI1640 Freedman, B.S., et al., Nature Communications. 2015 
Pluripotent stem cells FGF2, BMP4, Activin, BMP7, FGF9 DMEM/F12 Takasato, M., et al., Nautre. 2014 
Fetal tissue-derived cells BMP7, FGF2, LIF DMEM/F12 Li, Z., et al., Cell Stem Cell. 2016 
Lung

Lung

Cell type Relevant growth factors Relevant media Key publication
Pluripotent stem cells Activin, FGF4, FGF2, Noggin, SHH RPMI1640, Advanced DMEM/F12 Dye, BR., et al., Elife. 2015 
Pluripotent stem cells FGF2 Advanced DMEM/F12 Wilkinson, DC., et al., Stem Cells Translational Medicine. 2016 

Protocols for spheroid generation

To help guide you in your 3D cell culture, our R&D teams have developed suggested protocols for the generation of spheroids from the most commonly used cancer cell lines using Nunclon Sphera cell culture plastics and Gibco media.

Protocols for matrix-based 3D cell culture

For Research Use Only. Not for use in diagnostic procedures.