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There is a diverse range of protocols available in the literature around 3D cell culture and analysis. Discover those highlighted here to learn how researchers use Invitrogen and Gibco products in their methods.
We are now offering free e-Learns to share our expertise and assist with advancing your research. Follow the links below and start your free learning adventure in the basics of 3D cell culture and then progress to our neural organoid training course.
Getting started in 3D cell culture
Neural organoid generation from PSCs
Cell type | Relevant growth factors | Relevant media | Key publication |
---|---|---|---|
Pluripotent stem cells | Activin, FGF4, FGF2, Noggin, SHH | RPMI1640, Advanced DMEM/F12 | Dye, BR., et al., Elife. 2015 |
Pluripotent stem cells | FGF2 | Advanced DMEM/F12 | Wilkinson, DC., et al., Stem Cells Translational Medicine. 2016 |
Cell type | Relevant growth factors | Relevant media | Key publication |
---|---|---|---|
Pluripotent stem cells | Activin, FGF4, FGF2, Noggin, SHH | RPMI1640, Advanced DMEM/F12 | Dye, BR., et al., Elife. 2015 |
Pluripotent stem cells | FGF2 | Advanced DMEM/F12 | Wilkinson, DC., et al., Stem Cells Translational Medicine. 2016 |
To help guide you in your 3D cell culture, our R&D teams have developed suggested protocols for the generation of spheroids from the most commonly used cancer cell lines using Nunclon Sphera cell culture plastics and Gibco media.
For Research Use Only. Not for use in diagnostic procedures.