Participants must have access to an Applied Biosystems™ Real-Time PCR Instrument (7900HT Fast, QuantStudio™ series, 7500 series, StepOne™/StepOnePlus™, or ViiA™ 7 Real-Time PCR System), as well as materials necessary to set up a qPCR experiment (optical plates and adhesive covers, aerosol-resistant filter tips, pipettes, gloves, racks, vortex, microfuge tubes).
This class assumes attendees have prior knowledge of basic molecular biology concepts, including endpoint (conventional) PCR.