Discover Binding Site central nervous system (CNS) disorder-related assays

Optimize your workflow with our state-of-the-art CNS disorder-related assays including Freelite Mx™

Introduction

What are CSF & CNS disorders?

Cerebrospinal fluid (CSF) is a clear, colorless fluid that protects the central nervous system (CNS), which is comprised of the brain and spinal cord.

CSF is responsible for supplying nutrients and removing waste products to support cerebral metabolism. Changes in the composition, pressure, or color of the CSF can indicate the presence of specific medical conditions.

Intrathecal immunoglobulin synthesis refers to the production of immunoglobulins within the CSF. This process may occur in response to infections and inflammation of the central nervous system, or other neurological disorders.

Binding Site offer a complete solution of specialized assays, validated for CSF samples to characterize intrathecal immunoglobulin synthesis or help assess blood brain barrier function.

Complete Solution for CNS Assays

Discover the complete solution for CSF analysis available on the Optilite® special protein analyser

Designed to measure in paired cerebrospinal fluid (CSF) and serum samples.

Aid in the detection of intrathecal immunoglobin synthesis or blood brain barrier function.

  • Calibrated to international Standards (where one is available) 6 assays are available on Optilite for measurements in paired serum & cerebrospinal fluid (CSF) samples
  • Aids in characterizations of blood brain barrier function or intrathecal immunoglobin synthesis
Discover the full range of assays available on the Optilite® Analyser

CNS Assays

Detecting intrathecal immunoglobulin synthesis

The measurement of intrathecal immunoglobulin synthesis has been observed in various central nervous system disorders and is strongly associated with CNS inflammation and infections.1

There are several methods for detecting Intrathecal immunoglobulin synthesis by analysing the CSF:

  • Oligoclonal band analysis (OCB)
  • IgG in CSF
  • Kappa free light chains (κFLC) in CSF

OCB is a well established, gel-based technique to detect intrathecal immunoglobulin synthesis, however it can be challenging to perform:

  • Subjective: OCB requires subjective interpretation of the bands by an experienced technician, which can lead to inter-observer variability
  • Laborious: As a manual or semi-automated technique, OCB can be difficult and time-consuming to perform
  • Not always available: As a specialised technique, OCB is not always readily available in all laboratories  

IgG in CSF is another widely used technique. It has a quantitative and automated advantage over OCB as it can be readily performed on automated analyzers. However, this method has inconsistent agreement with OCB results.

Kappa free light chains (κFLC) in CSF is a newer biomarker for detecting intrathecal immunoglobulin synthesis.

It also has a quantitative and automated advantage over OCB as it can be readily performed on an automated platform such as the Optilite® analyzer, using the Freelite Mx™ Kappa assay from Binding Site. 

However, unlike measuring IgG in CSF, κFLC have excellent agreement to OCB while still overcoming the more challenging aspects of performing OCB. 

 

Freelite Mx™ assays to detect intrathecal immunoglobulin synthesis 

Freelite Mx™ assays may aid in the characterization of intrathecal immunoglobulins synthesis in CSF analysis, like oligoclonal band analysis.

This rapid, quantifiable, automated assay helps overcome some of the inherent difficulties in performing OCB and may help bring clarity to challenging cases.

  • Excellent agreement to OCB
  • Completely automated & quantitative
  • Non-subjective
  • Rapid 
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References
1. Carta S, et al. Oligoclonal bands: clinical utility and interpretation cues. Crit Rev Clin Lab Sci 2022; 1-14
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