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PureLink 套組
PureLink Purification Solutions
The Invitrogen PureLink nucleic acid purification kits are a family of purification products designed to meet the challenges posed by specific nucleic acid types, sample sources and volumes, throughput levels and downstream applications. These kits are based on either silica-membrane, anion exchange resin, or advanced silica-membrane purification technologies.
Learn more from our application notes:
PureLink 離心管柱套組
PureLink 離心管柱套組是快速、簡易且符合成本效益的核酸純化套組系列產品，可用以製備基因體 DNA、質體 DNA、總 RNA、微型 RNA，以及經福馬林固定石蠟包埋的 (FFPE) RNA。這些套組採用經證實的二氧化矽膜技術，並推翻含有繁瑣步驟的舊方法。對低處理量 (單一樣本) 和高處理量 (96 個和 384 個樣本) 的應用，我們均提供適用套組。
Obtain more RNA from your samples. RNA mini kits have 6 to 10 times the input capacity and higher yields compared to other suppliers’ kits.
| 核酸 | 應用 | 產品 |
|---|---|---|
| RNA | 動物與植物細胞、組織、全血、酵母菌和細菌的總 RNA | PureLink RNA 迷你套組 |
| 全血 | PureLink 總 RNA 血液套組 | |
| 細菌、酵母菌和植物與哺乳動物細胞的總 RNA - 96 孔 | PureLink 96 RNA 純化系統 | |
| 微型 RNA (miRNA) | PureLink miRNA 分離套組 | |
| 經福馬林固定石蠟包埋 (FFPE) 之樣本的總 RNA | PureLink FFPE RNA 分離套組 |
Figure 1. RNA yield from mouse brain tissue as determined by RNA analysis on a Qubit. Input volume of 150 mg for Qiagen not performed because it is above the 30 mg input capacity of the kit.
PureLink plasmid DNA kits are available is a variety of formats to best fit your downstream experiments. These range from mini and maxi kits for prokaryotic systems to low endotoxin kits for transfection into mammalian cells. Figure 2 illustrates how you can achieve high yields of endotoxin-free, advanced transfection–quality plasmid DNA with PureLink Expi as compared to kits from a competitor.
| 核酸 | 應用 | 產品 |
|---|---|---|
| 質體 DNA | 手動少量製備樣本 - 分子生物學級 | PureLink 快速質體少量製備套組 |
| 手動少量製備樣本 - 轉染級 | PureLink HQ 迷你質體純化套組 和 PureLink HQ 96 迷你質體純化套組 | |
| 高處理量質體少量製備 - 96 孔轉染級 | PureLink HQ 迷你質體純化套組 和 PureLink HQ 96 迷你質體純化套組 | |
| 大量製備與巨量製備 - 轉染級 | PureLink HiPure Expi 質體大量製備與巨量製備套組 | |
| 靈敏細胞株；轉染標準強健細胞株；所有分子生物學應用 | PureLink Fast 低內毒素質體少量與大量 |
Figure 2. Two high-copy plasmids with different backbones were purified using PureLink Expi and Qiagen EndoFree maxiprep, megaprep, and gigaprep kits as described in the product manuals. Data for DNA yields are shown in the graph; endotoxin values (EU/μg) are provided only for PureLink Expi preps.
PureLink genomic DNA (gDNA) kits isolate highly purified gDNA from a variety of samples including mammalian cells, bacteria, blood, and tissues. Figure 3 compares the results of gDNA isolated from cultured cells isolated using different suppliers.
| 核酸 | 應用 | 產品 |
|---|---|---|
| 基因體 DNA | 動物組織與細胞 | PureLink 基因體 DNA |
| 植物組織 | PureLink 基因體植物 DNA 純化套組 | |
| DNA | 瓊脂凝膠萃取 | PureLink 凝膠萃取套組 |
| PCR 反應淨化 | PureLink PCR 純化套組 |
Figure 3. Higher yields achieved with the PureLink Genomic DNA Mini Kit compared to those obtained with another supplier’s kit.
Obtain more RNA from your samples. RNA mini kits have 6 to 10 times the input capacity and higher yields compared to other suppliers’ kits.
| 核酸 | 應用 | 產品 |
|---|---|---|
| RNA | 動物與植物細胞、組織、全血、酵母菌和細菌的總 RNA | PureLink RNA 迷你套組 |
| 全血 | PureLink 總 RNA 血液套組 | |
| 細菌、酵母菌和植物與哺乳動物細胞的總 RNA - 96 孔 | PureLink 96 RNA 純化系統 | |
| 微型 RNA (miRNA) | PureLink miRNA 分離套組 | |
| 經福馬林固定石蠟包埋 (FFPE) 之樣本的總 RNA | PureLink FFPE RNA 分離套組 |
Figure 1. RNA yield from mouse brain tissue as determined by RNA analysis on a Qubit. Input volume of 150 mg for Qiagen not performed because it is above the 30 mg input capacity of the kit.
PureLink plasmid DNA kits are available is a variety of formats to best fit your downstream experiments. These range from mini and maxi kits for prokaryotic systems to low endotoxin kits for transfection into mammalian cells. Figure 2 illustrates how you can achieve high yields of endotoxin-free, advanced transfection–quality plasmid DNA with PureLink Expi as compared to kits from a competitor.
| 核酸 | 應用 | 產品 |
|---|---|---|
| 質體 DNA | 手動少量製備樣本 - 分子生物學級 | PureLink 快速質體少量製備套組 |
| 手動少量製備樣本 - 轉染級 | PureLink HQ 迷你質體純化套組 和 PureLink HQ 96 迷你質體純化套組 | |
| 高處理量質體少量製備 - 96 孔轉染級 | PureLink HQ 迷你質體純化套組 和 PureLink HQ 96 迷你質體純化套組 | |
| 大量製備與巨量製備 - 轉染級 | PureLink HiPure Expi 質體大量製備與巨量製備套組 | |
| 靈敏細胞株；轉染標準強健細胞株；所有分子生物學應用 | PureLink Fast 低內毒素質體少量與大量 |
Figure 2. Two high-copy plasmids with different backbones were purified using PureLink Expi and Qiagen EndoFree maxiprep, megaprep, and gigaprep kits as described in the product manuals. Data for DNA yields are shown in the graph; endotoxin values (EU/μg) are provided only for PureLink Expi preps.
PureLink genomic DNA (gDNA) kits isolate highly purified gDNA from a variety of samples including mammalian cells, bacteria, blood, and tissues. Figure 3 compares the results of gDNA isolated from cultured cells isolated using different suppliers.
| 核酸 | 應用 | 產品 |
|---|---|---|
| 基因體 DNA | 動物組織與細胞 | PureLink 基因體 DNA |
| 植物組織 | PureLink 基因體植物 DNA 純化套組 | |
| DNA | 瓊脂凝膠萃取 | PureLink 凝膠萃取套組 |
| PCR 反應淨化 | PureLink PCR 純化套組 |
Figure 3. Higher yields achieved with the PureLink Genomic DNA Mini Kit compared to those obtained with another supplier’s kit.
如需本公司質體純化產品的其他資訊，請前往質體 DNA 分離
Select peer-reviewed publications
- Sathiamoorthy, S., Malott, R. J., Gisonni-Lex, L., et al. (2018). Selection and evaluation of an efficient method for the recovery of viral nucleic acid extraction from complex biologicals. NPJ vaccines, 3(1), 31.
- Bunker, J. J., Erickson, S. A., Flynn,et al. (2017). Natural polyreactive IgA antibodies coat the intestinal microbiota. Science, 358(6361), eaan6619.
- Solanas, G., Peixoto, F. O., Perdiguero, et al. (2017). Aged stem cells reprogram their daily rhythmic functions to adapt to stress. Cell, 170(4), 678-692.
- Sykes, D. B., Kfoury, Y. S., Mercier, F. Eet al. (2016). Inhibition of dihydroorotate dehydrogenase overcomes differentiation blockade in acute myeloid leukemia. Cell, 167(1), 171-186.
- Miyamoto T, Okano S, Kasai N. (2009) Irreversible thermoinactivation of ribonuclease-A by soft-hydrothermal processing. Biotechnol Prog. 25:1678-1685.
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For Research Use Only. Not for use in diagnostic procedures.