PureLink® Total RNA Blood Kit

Catalog number: K156001

Ambion™  Related applications: RNA Extraction

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Description

PureLink® Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 µl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Simple RNA Purification from Whole Blood
PureLink® Total RNA Blood Kit is designed for purifying from 50 to 500 µl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink® Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink® Total RNA Blood Kit.

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
Northern Blotting Kits & Reagents
Nuclease protection assays
Microarray
• cDNA synthesis
Real-Time PCR (qPCR)

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction, Nuclease Protection Assays, Microarray Analysis, Real-Time Quantitative PCR (qPCR)
Scale: Mini
Format: Spin Column
System: PureLink™
Quantity: 50
Product Size: 50 preps
Final Product: Total RNA
Yield (up to): 1000 µg
Purification Time: 20 min
Number of Reactions: 50 Preps
Isolation Technology: Silica Spin Column
Starting Material (Amount): 50 to 500 µl blood, Up to 3.5 x 10^6 leukocytes
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature.

Documents

Manuals & protocols

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