PureLink® Total RNA Blood Kit

Catalog number: K156001

Ambion™  Related applications: RNA Extraction

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PureLink® Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 µl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Simple RNA Purification from Whole Blood
PureLink® Total RNA Blood Kit is designed for purifying from 50 to 500 µl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink® Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink® Total RNA Blood Kit.

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
Northern Blotting Kits & Reagents
Nuclease protection assays
• cDNA synthesis
Real-Time PCR (qPCR)

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Blood
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Northern Blotting
Scale: Mini
Format: Spin Column
System: PureLink™
Quantity: 50
Product Size: 50 preps
Final Product: Total RNA
Yield (up to): 1000 µg
Purification Time: 20 min
Number of Reactions: 50 Preps
Isolation Technology: Silica Spin Column
Starting Material (Amount): 50 to 500 µl blood, Up to 3.5 x 10^6 leukocytes
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature.


Manuals & protocols

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