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Invitrogen™

PureLink™ Total RNA Blood Kit

Catalog number: K156001
Invitrogen™

PureLink™ Total RNA Blood Kit

Catalog number: K156001
Catalog Number
K156001
also known as K1560-01
Unit Size
50 preps
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K156001
also known as K1560-01
50 preps
-
Product Overview
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PureLink™ Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 μl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Simple RNA Purification from Whole Blood
PureLink™ Total RNA Blood Kit is designed for purifying from 50 to 500 μl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink™ Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink™ Total RNA Blood Kit.

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
Northern Blotting Kits & Reagents
Nuclease protection assays
Microarray
• cDNA synthesis
Real-Time PCR (qPCR)

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Silica Spin Column
Purification Time
20 min. (After sample homogenization)
Elution Volume
30–100 μL
Sample Type
Blood
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity
50 Preps
Scale
Mini
Shipping Condition
Room Temperature
Starting Material Amount
Blood: ≤500 μL
Yield
1000 μg (Binding capacity)

Contents & Storage

• 17.5 mL Lysis Buffer L3; store at room temperature
• 175 mL Lysis Buffer L5; room temperature
• 60 mL Wash Buffer W4; room temperature
• 10 mL Wash Buffer W5; room temperature
• 5 mL RNase-free Water; room temperature
• 50 Spin Cartridges with Collection Tubes; room temperature
• 50 Wash Tubes (2 mL); room temperature
• 50 Elution Tubes (1.7 mL); room temperature

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