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Invitrogen™

PureLink™ Pro 96 total RNA Purification Kit

Catalog number: 12173011A
Invitrogen™

PureLink™ Pro 96 total RNA Purification Kit

Catalog number: 12173011A
Catalog Number
12173011A
also known as 12173-011A
Unit Size
4 plates
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12173011A
also known as 12173-011A
4 plates
-
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The PureLink™ Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink™ Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Isolation Technology
Silica Plate
Purification Time
25 min. (After sample homogenization)
Elution Volume
45–170 μL
Sample Type
Bacteria, Cells, Plant, Tissue, Yeast
Final Product Type
Total RNA
High-throughput Compatibility
High-throughput Compatible
For Use With (Equipment)
Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity
4 Plates
Scale
Mini
Shipping Condition
Room Temperature
Starting Material Amount
Bacteria: ≤109 cells
Cells: varies
Plant: ≤150 mg
Tissue: ≤100 mg
Yeast: ≤1.8 mL
Yield
Up to 33 μg

Contents & Storage

• 150 mL PureLink Pro 96 Lysis Buffer; store at room temperature
• 250 mL PureLink Pro 96 Wash Buffer I; room temperature
• 250 mL PureLink Pro 96 Wash Buffer II; room temperature
• 75 mL PureLink Pro 96 RNase-free Water; room temperature
• 4 PureLink 96-well Total RNA Filter Plates; room temperature
• 4 PureLink Pro 96 Elution Plates; room temperature

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