PureLink® Pro 96 total RNA Purification Kit

Catalog number: 12173011A

Ambion™  Related applications: RNA Extraction

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Description

The PureLink® Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink® Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria, Blood, Tissue, Plant Samples, Cells, Yeast
High Throughput Compatibility: High Throughput-Compatible
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Northern Blotting, Real-Time Quantitative PCR (qPCR), Nuclease Protection Assays
Scale: Mini
Format: 96-well plate
System: PureLink™
Quantity: 1 kit
Product Size: 4 plates
Final Product: Total RNA
Yield (up to): 33 µg
Purification Time: 20 min
Number of Reactions: 96 Preps
Elution Volume - max: 150 µl
Elution Volume - min: 100 µl (vacuum), 50 µl (centrifuge)
Isolation Technology: Silica Plate
Sample Type (Specific): Blood, Cell Cultures, Bacteria, Insect Cells, Plant, Tissue (Mammalian), Yeast, Mammalian Cells
Purity or Quality Grade: Molecular Biology Grade
For Use With (Equipment): Biomek® FX, Eppendorf epMotion, Robotic Liquid Handling Systems, Vacuum Manifold, TECAN Freedom Evo, TECAN Genesis, Centrifuge
Shipping Condition: Room Temperature

Contents & storage

The PureLink® Pro 96 total RNA Purification Kit is supplied includes PureLink 96 Well Total RNA Filter Plates, elution plates, lysis buffer, Wash Buffers I and II, and RNase-free water. Components are also available separately. Store at room temperature. Guaranteed stable for 2 years when properly stored.

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