PureLink&trade; <i>Pro</i> 96 total RNA Purification Kit
Invitrogen™

PureLink™ Pro 96 total RNA Purification Kit

The PureLink Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes.
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KatalognummerMengeProdukttyp
12173011A4 PlattenRNA-Reinigungskit
12173022750 mLRNA-Lysepuffer
12173033
auch als 12173-033 bezeichnet
2 x 1 lRNA Waschpuffer II (5X)
12173032
auch als 12173-032 bezeichnet
5 x 1 lRNA-Waschpuffer I
Katalognummer 12173011A
Preis (EUR)
1.916,00
Each
Menge:
4 Platten
Produkttyp:
RNA-Reinigungskit
Preis (EUR)
1.916,00
Each
The PureLink Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.

The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design

The PureLink Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elutionsvolumen45 bis 170 μl
EndprodukttypGesamt-RNA
Zur Verwendung mit (Anwendung)RT-PCR, qPCR, cDNA-Bibliothekskonstruktion, NGS, Microarray-Analyse, Blot-Hybridisierung, Northern Blotting, In-vitro-Translation, Nuclease-Protection-Assays, Nukleinsäure-Markierung
Zur Verwendung mit (Geräte)Biomek™ FX, Robotic Liquid-Handling-Systeme, Vakuumkammer, Eppendorf epMotion™, Zentrifuge, TECAN Freedom Evo™, Tecan Genesis™
Hochdurchsatz-KompatibilitätGeeignet für hohe Durchsätze
ProdukttypRNA-Reinigungskit
Aufreinigungszeit25 Min. (nach Probenhomogenisierung)
Menge4 Platten
UmfangMini
VersandbedingungRaumtemperatur
AusgangsmaterialmengeBakterien: ≤109 Zellen
Zellen: variiert
Pflanze: ≤ 150 mg
Gewebe: ≤ 100 mg
Hefe: ≤1,8 ml
ErtragBis zu 33 μg
Isolation TechnologyKieselgelplatte
ProbentypBakterien, Zellen, Pflanzen, Gewebe, Hefe, Blutproben, Zellkulturen, Insektenzellen, Hefe
Unit SizeEach
Inhalt und Lagerung
• 150 ml PureLink Pro 96 Lysepuffer; bei Raumtemperatur lagern
• 250 ml PureLink Pro 96 Waschpuffer I; Raumtemperatur
• 250 ml PureLink Pro 96 Waschpuffer II; Raumtemperatur
• 75 ml PureLink Pro 96 RNase-freies Wasser; Raumtemperatur
• 4 PureLink 96-Well Gesamt-RNA-Filterplatten; Raumtemperatur
• 4 PureLink Pro 96 Elutionsplatten; Raumtemperatur

Häufig gestellte Fragen (FAQ)

If I do not use all the wells in the filter plate of the PureLink Pro 96 Purification System, can I use those wells at another time?

Yes. Keep the unused wells covered with Foil Tape. Please note that extra Foil Tape and Receiver Plates will be needed.

My centrifuge does not reach 3,000 x g. For the PureLink Pro 96 system, could I spin at a lower force for a longer time?

Generally speaking, yes. It should work the same if you can keep the same g (rcf) x time.