PureLink® Pro 96 total RNA Purification Kit

Catalog number: 12173011A

Ambion™  Related applications: RNA Extraction

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Description

The PureLink® Pro 96 total RNA Purification Kit provides high-throughput isolation of high-quality total RNA from the widest range of sample types and sizes. Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples (Figures 1 and 2).
The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink® Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates(Figure 3) for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria, Blood, Cells, Plant Samples, Tissue, Yeast
High Throughput Compatibility: High Throughput-Compatible
Downstream Application: Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
Elution Volume - max: 150 µl
Elution Volume - min: 100 µl (vacuum), 50 µl (centrifuge)
Final Product: Total RNA
For Use With (Equipment): Biomek® FX, Centrifuge, Eppendorf epMotion, Robotic Liquid Handling Systems, TECAN Freedom Evo, TECAN Genesis, Vacuum Manifold
Format: 96-well plate
Isolation Technology: Silica Plate
Number of Reactions: 96 Preps
Product Line: PureLink™
Product Size: 4 plates
Purification Time: 20 min
Purity or Quality Grade: Molecular Biology Grade
Quantity: 1 kit
Sample Type (Specific): Bacteria, Blood, Cell Cultures, Insect Cells, Mammalian Cells, Plant, Tissue (Mammalian), Yeast
Scale: Mini
System: PureLink™
Yield (up to): 33 µg
Shipping Condition: Room Temperature

Contents & storage

The PureLink® Pro 96 total RNA Purification Kit is supplied includes PureLink 96 Well Total RNA Filter Plates, elution plates, lysis buffer, Wash Buffers I and II, and RNase-free water. Components are also available separately. Store at room temperature. Guaranteed stable for 2 years when properly stored.

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