PureLink™ miRNA Isolation Kit
PureLink™ miRNA Isolation Kit
Invitrogen™

PureLink™ miRNA Isolation Kit

The Ambion™ PureLink™ miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA)Read more
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Catalog NumberQuantity
K15700125 preps
Catalog number K157001
Price (USD)/ Each
321.65
Online Exclusive
335.00
Save 13.35 (4%)
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Quantity:
25 preps
Price (USD)/ Each
321.65
Online Exclusive
335.00
Save 13.35 (4%)
Add to cart
The Ambion™ PureLink™ miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol. The kit provides a rapid and convenient method for purifying small RNA molecules from animal and plant cells, as well as from bacteria and yeast samples.
• Purify small RNA molecules with a novel enrich/bind/elute procedure
• Prepare enriched microRNA samples typically in less than 15 minutes
• Minimize contaminating large RNA molecules and genomic DNA

Rapid and Convenient Column Purification Procedure for small RNAs
The Ambion™ PureLink™ miRNA Isolation Kit is specifically designed to purify small RNA molecules, including miRNAs and siRNAs, from a variety of samples typically in less than 15 minutes (see Table 1). The kit utilizes a silica-based two-column system to enrich exclusively for small RNAs (see Figure 1). RNAs greater than approximately 200 nucleotides are bound to the first column. Ethanol is then added to the flow-through in order to increase the binding affinity of small RNAs to the glass fiber membrane in the second column. Once bound to the second column, small RNA molecules are then eluted, ready-for-use, and free of large RNA molecules such as 28S rRNA, 18S rRNA, and mRNA, allowing for focused expression analysis studies of small RNA molecules.

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
The miRNA purified using the Ambion™ PureLink™ miRNA Isolation Kit is suitable for downstream applications including RNA sequencing, microarray analysis, real-time PCR (qPCR), reverse transcription real-time PCR (RT-qPCR), and northern blotting.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume50–100 μL
Final Product TypeSmall RNA
For Use With (Application)qRT-PCR, Microarray Analysis, Northern Blotting
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time20 min. (After sample homogenization)
Quantity25 preps
ScaleMini
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤106 cells
Cells: 106
Plant: varies
Tissue: ≤100 mg
Yeast: 1 mL
Dicing Reaction: 300 μL
YieldUp to 6 μg
Isolation TechnologySilica Spin Column
Sample TypeBacteria, Cells, Plant, Tissue, Yeast, Dicing Reaction
Unit SizeEach
Contents & Storage
• 45 mL Binding Buffer L3; store at room temperature
• 10 mL Wsh Buffer W5; room temperature
• 3 mL Buffer W4; room temperature
• 5 mL RNase-free Water; room temperature
• 50 Spin Cartridges with Collection Tubes; room temperature
• 50 Wash Tubes (2 mL); room temperature
• 50 Recovery Tubes (1.7 mL); room temperature