PureLink™ FFPE RNA Isolation Kit

Catalog number: K156002

Ambion™  Related applications: RNA Extraction

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Description

The PureLink® FFPE RNA Isolation Kit provides a simple, reliable, and rapid method to isolate high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens for retrospective basic and clinical biological studies of gene expression without the use of toxic organic solvents for deparaffinization.
• Offers a rapid and environmentally friendly procedure for the extraction of RNA from FFPE tissue specimens
• Isolates RNA with maximum yield and minimum degradation

Rapid and Non-Toxic Isolation Procedure
The PureLink® FFPE RNA Isolation Kit employs a simple heating procedure for deparaffinization, instead of relying on the use of toxic organic solvents such as xylene. RNA isolation can be completed in 30 - 40 minutes instead of days, as is required for older FFPE isolation methods (Figure 1). The heating procedure for deparaffinization partly restores RNA template structure for cDNA synthesis and improves template quality for reverse transcription.

High Yield, High Quality RNA
The PureLink® FFPE RNA Isolation Kit offers higher yields and purity as compared to other commercially available RNA purification systems, allowing for RT-PCR products over 1 kb in length (Figure 2). In addition, the PureLink® FFPE RNA Isolation Kit minimizes genomic DNA contamination of the purified RNA sample and provides an option for DNase digestion.

Purified Total RNA is Suitable for Use with a Variety of Downstream Applications and Products
RNA extracted from FFPE specimens is suitable for downstream applications such as reverse transcription, real-time PCR (qPCR), real-time RT-PCR (qRT-PCR), and microarray analysis.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): FFPE & Fixed Samples
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Microarray Analysis, Real-Time Quantitative PCR (qPCR), Northern Blotting, cDNA Library Construction
Scale: Mini
Format: Spin Column
System: PureLink™
Quantity: 50
Product Size: 50 preps
Final Product: Total RNA
Yield (up to): 1 µg
Purification Time: 30 min
Number of Reactions: 50 Preps
Isolation Technology: Silica Spin Column
Sample Type (Specific): Tissue (FFPE)

Contents & storage

The PureLink™ FFPE RNA Isolation Kit is sufficient for up to 50 reactions and each reaction is capable of purifying total RNA for up to 10 slices of 10 µm-thick FFPE sections. Materials provided in the kit include: Deparaffinization/Protinase K digestion buffer, Protein/RNase Degrading buffer, 50 spin columns, 50 wash tubes, 50 recovery tubes, Binding buffer, and Wash buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols

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