The most complete fluorescent labeling and detection reference available, The Molecular Probes Handbook—A Guide to Fluorescent Probes and Labeling Technologies contains over 3,000 reagents and kits representing a wide range of Invitrogen Molecular Probes labeling and detection products. The significantly revised 11th edition features extensive references, reorganized content, and new technical notes and product highlights.

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Table of contents

Fluorescence Fundamentals

Part I—Choosing a fluorescent label: Their properties and labeling chemistries

Chapter 1 — Fluorophores and Their Amine-Reactive Derivatives
Chapter 2 — Thiol-Reactive Probes
Chapter 3 — Click Chemistry and Other Functional Group Modifications
Chapter 4 — Biotin and Hapten Derivatives
Chapter 5 — Crosslinking and Photoactivatable Reagents

Part II—Detecting biomolecules: Signal amplification and secondary detection

Chapter 6 — Ultrasensitive Detection Technology
Chapter 7 — Antibodies, Avidins and Lectins

Part III—Decoding cell components: Nucleic acid and protein analysis

Chapter 8 — Nucleic Acid Detection and Analysis
Chapter 9 — Protein Detection and Analysis
Chapter 10 — Enzyme Substrates and Assays

Part IV—Probing cell structure: From organelles and membranes to whole cells

Chapter 11 — Probes for Cytoskeletal Proteins
Chapter 12 — Probes for Organelles
Chapter 13 — Probes for Lipids and Membranes
Chapter 14 — Fluorescent Tracers of Cell Morphology and Fluid Flow

Part V—Probing cell function: From cell viability to ion flux

Chapter 15 — Assays for Cell Viability, Proliferation and Function
Chapter 16 — Probes for Endocytosis, Receptors and Ion Channels
Chapter 17 — Probes for Signal Transduction
Chapter 18 — Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19 — Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20 — pH Indicators
Chapter 21 — Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22 — Probes for Membrane Potential

Part VI—Integrating technology and instrumentation

Chapter 23 — Antifades and Other Tools for Fluorescence Applications

Supplementary information

Technical Notes and Product Highlights
List of Tables 

Resources

Technical resources

Cell Analysis Support Center
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