Intact Virus Precipitation Reagent (optimized for SARS-CoV-2) enables fast and efficient enrichment of intact, live SARS-CoV-2 coronavirus from dilute cell culture and virus transport media. The enrichment is gentle and fast and can be performed in less than three hours. By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The isolated intact virus can be used for functional studies, in contrast to current SARS-CoV-2 purification methods based on extracting RNA for detection purposes. Intact Virus Precipitation Reagent allows for enrichment of intact virus from large, dilute samples for further virus growth or use in any downstream functional assays, protein research, PCR, etc.

Why use Intact Virus Precipitation Reagent (optimized for SARS-CoV-2)
• Simple—3-step SARS-CoV-2 enrichment requiring only a centrifuge
• Fast—enrichment in <3 hours with minimal hands-on time
• Flexible volume—dilute virus samples can be enriched from large sample volumes
• Straight to PCR—no further nucleic acid extraction is needed prior to PCR

How to use Intact Virus Precipitation Reagent (optimized for SARS-CoV-2)
Use twice as much virus sample as precipitation reagent (e.g., use 2 mL virus sample per 1 mL reagent).

1. Add precipitation reagent to the virus sample and incubate for 2 hours at 2–8°C.
2. Centrifuge for 30 minutes at 10,000 x g.
3. Remove supernatant and keep the pellet with the enriched virus.

The enriched virus is now ready for any downstream analysis and can be used directly in a PCR reaction without any further nucleic acid extraction.

For higher throughput automation needs for intact SARS-CoV-2, use the magnetic bead-based Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2) in combination with one of our KingFisher instruments.

The Covid-19 pandemic has led to an explosion of SARS-CoV-2 detection with PCR and sequencing. But SARS-CoV-2 research has a growing need to understand how the virus works, how it influences the body and immune system, how the virus evolves over time, and how to develop new vaccines and therapies. This increases the need to study the intact virus. In addition, virus-like particles (VLPs), containing only virus-specific proteins and not nucleic acids, are extensively used in viral proteomic research. Intact Virus Precipitation Reagent (optimized for SARS-CoV-2) isolates both live and inactivated SARS-CoV-2 intact virus and VLPs. The reagent can also be used on other viruses.

If you are in a process of customizing Intact Virus Precipitation Reagent (optimized for SARS-CoV-2) in a commercial product or service, or you need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com.