• 500 µL 1 Kb Plus DNAラダー • 1 mL 10X BlueJuiceゲルローディングバッファー
-20℃で保管してください。
よくあるご質問(FAQ)
Can I know the sequences of Invitrogen DNA ladders?
Sequences of Invitrogen DNA and RNA ladders are proprietary.
Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?
Invitrogen DNA ladders contain linear dsDNA fragments.
Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?
Invitrogen DNA ladders are composed of double-stranded DNA fragments only.
Why are the DNA bands from my molecular weight ladder smearing?
Here are a few reasons why you might see smearing of the bands:
- The DNA was degraded. Avoid nuclease contamination of DNA standards.
- Too much DNA was loaded on the gel. Decrease the amount of DNA in the gel.
- The DNA was contaminated by protein. Remove proteins by phenol extraction before electrophoresis.
- For small DNA, the bands may have diffused during staining. Add the ethidium bromide before electrophoresis.
- For radiolabeled DNA, labeling was performed by nick translation. Label the DNA by replacement synthesis with T4 DNA polymerase or label the 5' end with T4 polynucleotide kinase.
- Improper electrophoresis conditions were used. Do not allow voltage to exceed ~20 V/cm. Maintain a temperature <30°C during electrophoresis. Check that the electrophoresis buffer used has sufficient buffering capacity.
- The DNA contained too much salt. Remove excess salt by ethanol precipitation before electrophoresis.
I'm seeing anomalous migration of my DNA ladder. What happened?
This can happen if the marker was heated. Please ensure that the ladders are not heated before use.
Although the papillomavirus structural proteins, L1 and L2, can spontaneously coassemble to form virus-like particles, currently available methods for production of L1/L2 particles capable of transducing reporter plasmids into mammalian cells are technically demanding and relatively low-yield. In this report, we describe a simple 293 cell transfection method for efficient ... More
Activation of protein kinase C beta II by the stereo-specific phosphatidylserine receptor is required for phagocytosis of apoptotic thymocytes by resident murine tissue macrophages.
We showed previously that protein kinase C (PKC) is required for phagocytosis of apoptotic leukocytes by murine alveolar (AMø) and peritoneal macrophages (PMø) and that such phagocytosis is markedly lower in AMø compared with PMø. In this study, we examined the roles of individual PKC isoforms in phagocytosis of apoptotic ... More
Bisindolylmaleimide VIII enhances DR5-mediated apoptosis through the MKK4/JNK/p38 kinase and the mitochondrial pathways.
Authors: Ohtsuka Toshiaki; Zhou Tong;
Journal:J Biol Chem
PubMed ID:12034736
Bisindolylmaleimide VIII (Bis VIII) has been previously shown to enhance Fas-mediated apoptosis through a protein kinase C-independent mechanism. In the present study, we examined the effect of Bis VIII on apoptosis induced by DR5 (TRAIL-R2), using an agonistic anti-human DR5 monoclonal antibody, TRA-8. Our results demonstrated that Bis VIII was ... More