Citas y referencias (2)

Gibco™

Medio de Waymouth

Waymouth's is a chemically defined medium developed by Charity Waymouth and originally formulated for studies on the nutrition, metabolism, andMás información

Número de catálogo	Cantidad
11220035	500 mL

Número de catálogo 11220035

Precio (MXN)

Cantidad:

500 mL

Customize this product

Waymouth's is a chemically defined medium developed by Charity Waymouth and originally formulated for studies on the nutrition, metabolism, and growth of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Waymouth's medium is also used for the culture of whole organs and a broad range of cell types, such as carcinoma cell lines.

This Waymouth's medium is modified as follows:

With	Without
• L-glutamine	• HEPES
• Phenol Red

The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Especificaciones

Línea de célulasL929, carcinomas

Tipo de célulaórganos completos

Concentración1X

Calidad de fabricacióncGMP-compliant under the ISO 13485 standard

Línea de productosGibco

Tipo de productoMedio de Waymouth

Cantidad500 mL

Duración de almacenamiento12 meses a partir de la fecha de fabricación

Condiciones de envíoTemperatura ambiente

ClasificaciónLibre de material de origen animal

FormularioLíquido

EsterilidadEstéril con filtro

Con aditivosAlto contenido en glucosa, Glutamina, Rojo de fenol

Sin aditivosSin HEPES, Sin piruvato sódico

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medio de Waymouth FAQs

Citations & References (2)

Citations & References

Abstract

Ets-1 positively regulates Fas ligand transcription via cooperative interactions with Sp1.

Authors:Kavurma MM, Bobryshev Y, Khachigian LM,

Journal:J Biol Chem

PubMed ID:11970950

'The FasL/Fas system has been implicated in smooth muscle cell apoptosis and atherosclerotic plaque instability, a process that can lead to plaque rupture, precipitating myocardial infarction and sudden death. The transcriptional mechanisms regulating FasL gene expression in vascular smooth muscle cells are poorly understood. We recently described a novel mechanism ... More

Intracellular third loop domain of angiotensin II type-2 receptor. Role in mediating signal transduction and cellular function.

Authors: Hayashida W; Horiuchi M; Dzau V J;

Journal:J Biol Chem

PubMed ID:8703004

'The present study tests the hypothesis that the unique intracellular third loop domain of angiotensin II type-2 (AT2) receptor is essential for the subsequent intracellular signaling and plays an important role in mediating receptor function. Synthetic intracellular third loop peptide of the AT2 receptor (AT2-3LP, 22 amino acids) and control ... More