11598018 - FAQs

Which promoter, T7 or SP6, should be used for an in vitro transcription/translation of clones in the pCMVSport vectors that were used to generate Invitrogen premade cDNA libraries?

The cDNA insert is cloned in the Not I/Sal I sites of the pCMVSport vectors. The Sal I site is the 5' end of the sense strand. Therefore, in order to make an in vitro transcribed/translated product from the sense strand, one would have to use the SP6 promoter.