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SuperScript V VILO Master Mix
Invitrogen™

SuperScript V VILO Master Mix

Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications.
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Catalog NumberNo. of ReactionsIncludes
1180011150 ReactionsSuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
1180000150 ReactionsSuperScript V VILO Master Mix, No RT Control, Water
11800002500 ReactionsSuperScript V VILO Master Mix, No RT Control, Water
118000032000 ReactionsSuperScript V VILO Master Mix, No RT Control, Water
11800112500 ReactionsSuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
118001132000 ReactionsSuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
118000041250 ReactionsSuperScript V VILO Master Mix only
Catalog number 11800111
Price (USD)
800.00
Each
No. of Reactions:
50 Reactions
Includes:
SuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
Price (USD)
800.00
Each

Invitrogen™ SuperScript™ V VILO™ Master Mix is a ready-to-use master mix designed for fast, sensitive, and reproducible cDNA synthesis in two-step RT-qPCR applications. Powered by SuperScript™ V Reverse Transcriptase, the optimized master mix combines robust performance across a broad range of RNA samples with simplified reaction setup, enhanced reagent stability, and greater workflow flexibility. SuperScript V VILO Master Mix is also available with ezDNase Master Mix for fast and convenient genomic DNA removal prior to reverse transcription.

Features of SuperScript V VILO Master Mix include:

  • Broad RNA input range—supports sensitive cDNA synthesis from 0.1 pg to 5 μg of total RNA
  • Robust performance with challenging RNA—efficient cDNA synthesis from highly structured, GC-rich, degraded and inhibitor-containing RNA
  • Fast cDNA synthesis—complete reverse transcription in as little as 1-10 minutes
  • Enhanced reagent stability—supports ambient shipping and room-temperature storage for up to 1 month
  • Simple, flexible workflow—ready-to-use format simplifies reaction setup, while room-temperature assembly and extended stability of assembled reactions enable greater workflow flexibility

SuperScript V VILO Master Mix combines SuperScript V RT, RNase inhibitor, buffer, dNTPs, and an optimized blend of oligo(dT) and random primers in a single formulation, simplifying reaction setup and helping reduce variability. Its combination of fast cDNA synthesis, robust performance, and flexible room-temperature handling makes it well suited for routine, high-throughput, and automated RT-qPCR workflows.

SuperScript V VILO Master Mix with gDNA Removal allows streamlined genomic DNA removal step. The dsDNA-specific ezDNase enzyme removes contaminating genomic DNA in as little as 2 minutes without affecting RNA, further simplifying the two-step RT-qPCR workflow.

A DTT-free, animal origin-free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeFirst-Strand cDNA
FormatMaster Mix
IncludesSuperScript V VILO Master Mix, No RT Control, ezDNase Master Mix, Water
Inhibitor Tolerance (Simple)High
No. of Reactions50 Reactions
Optimal Reaction Temperature50°C
Product TypeReverse Transcription Reagent
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript V
Ribonuclease H ActivityReduced
Sensitivity0.1 pg total RNA
Shipping ConditionDry Ice, Wet Ice, Ambient
Size (Final Product)Up to 10 kb
Starting MaterialRNA
TechniqueReverse Transcription
ThermostabilityUp to 65°
Concentration5X
Detection MethodqPCR, NGS, PCR
For Use With (Application)Gene Expression, Real Time PCR (qPCR), RT-PCR, cDNA Synthesis
FormLiquid
GC-Rich PCR PerformanceHigh
Label or DyeNot Applicable
PCR Method2-step RT-qPCR, RT-PCR
Reaction Speed10 min.
Unit SizeEach
Contents & Storage
• SuperScript V VILO Master Mix (200 μL)
• SuperScript V VILO No RT Control (100 μL)
• ezDNase Master Mix (50 μL)
• Nuclease-free water (1.25 mL)

Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 5 months
• Room temperature for up to 1 month

Frequently asked questions (FAQs)

What are the main differences between Invitrogen SuperScript V Reverse Transcriptase and Invitrogen SuperScript V VILO Master Mix?

Invitrogen SuperScript V Reverse Transcriptase is supplied as a stand-alone enzyme with reaction buffer and supports flexible reaction optimization. Invitrogen SuperScript V VILO Master Mix combines all required reaction components into a single formulation to simplify workflow setup and reduce pipetting steps.

Can Invitrogen SuperScript V Reverse Transcriptase products support automated workflows?

Yes. Invitrogen SuperScript V Reverse Transcriptase products include features such as room-temperature reaction setup, benchtop stability of assembled reactions, and simplified workflow options that can support automated and high-throughput workflows.

Can Invitrogen SuperScript V Reverse Transcriptase products support low-input RNA workflows?

Yes. Invitrogen SuperScript V Reverse Transcriptase products are designed to support reverse transcription from low-input RNA samples, including inputs as low as 0.1 pg of total RNA.

Which Invitrogen SuperScript V Reverse Transcriptase product should I choose for RT-qPCR workflows?

For two-step RT-qPCR workflows, Invitrogen SuperScript V VILO Master Mix reagent (Cat. No. 11800001) provides an all-in-one formulation that simplifies reaction setup. Users who prefer custom primer selection may choose Invitrogen SuperScript V VILO Master Mix, Primer-Free (Cat. No. 11900001) or Invitrogen SuperScript V Reverse Transcriptase (Cat. No. 19090050).

Can reaction volumes be scaled when using Invitrogen SuperScript V Reverse Transcriptase products?

Yes. Reaction volumes may be scaled up to 100 µL when using Invitrogen SuperScript V Reverse Transcriptase products provided that reagent ratios are maintained and workflow performance is verified.

View all SuperScript V VILO Master Mix FAQs