The Gateway™ Vector Conversion System is designed to convert any vector into a Gateway™ destination vector using restriction endonucleases and ligase. A Gateway™ cassette containing attR recombination sites flanking a ccdB gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway™ vectors containing the ccdB gene. The Gateway™ Vector Conversion System offers: • Conversion of any expression vector into a Gateway™ destination vector • Three cassettes to construct a destination vector in the correct reading frame. Each cassette has a unique restriction site to easily distinguish the cassettes and screen for the correct orientation.
For Research Use Only. Not for use in diagnostic procedures.
Vector Conversion System
Antibiotic Resistance Bacterial
Bacterial or Yeast Strain
ccdB Survival™ 2
Contents & Storage
The Gateway™ Vector Conversion System contains Gateway™ Reading Frame Cassettes A, B, and C.1, pENTR™-gus positive control, and One Shot™ ccdB Survival Cells. Store DNA at -20°C and competent cells at -80°C.