Sulfuro de estreptomicina
Sulfuro de estreptomicina
Citas y referencias (5)
Gibco™

Sulfuro de estreptomicina

El sulfato de estreptomicina es un antibiótico soluble en agua originalmente purificado a partir de Streptomyces griseus.El sulfuro de estreptomicinaMás información
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Número de catálogoCantidad
11860038100 g
Número de catálogo 11860038
Precio (USD)
169,50
Each
Añadir al carro de la compra
Cantidad:
100 g
Precio (USD)
169,50
Each
Añadir al carro de la compra
El sulfato de estreptomicina es un antibiótico soluble en agua originalmente purificado a partir de Streptomyces griseus.El sulfuro de estreptomicina actúa uniéndose a la subunidad 30S del ribosoma bacteriano, lo que conduce a la inhibición de la síntesis de proteínas y la muerte de bacterias susceptibles.El sulfato de estreptomicina es altamente activo contra gramnegativos con alguna actividad contra bacterias grampositivas.El sulfato de estreptomicina Gibco™ se utiliza solo o en combinación con la penicilina, un antibiótico altamente activo contra bacterias grampositivas, para la prevención de la contaminación bacteriana de cultivos celulares.La concentración de trabajo recomendada varía entre 50 y 100 μg/ml.Ofrecemos una gran variedad de antibióticos y antimicóticos para aplicaciones de cultivos celulares.

Uso del producto
Para uso exclusivo en investigación:No diseñado para uso diagnóstico o terapéutico en animales o humanos.

Sistema de fabricación según las buenas prácticas de fabricación actuales en dos instalaciones
Para la continuidad de la cadena de suministro, fabricamos el sulfuro de estreptomicina Gibco™ en dos instalaciones distintas ubicadas en Grand Island (Nueva York, EE. UU.) y Escocia (Reino Unido).Ambos sitios son conformes con los requisitos de producción según las buenas prácticas de fabricación actuales, cuentan con la certificación ISO 13485 y están registrados en la FDA como fabricantes de dispositivos médicos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ConcentraciónDe 50 a 100 μg/ml
Tipo de cultivoCultivo celular de mamíferos, cultivo celular de insectos
Para utilizar con (aplicación)Selección bacteriana
Cantidad100 g
Duración de almacenamiento24 meses
Condiciones de envíoTemperatura ambiente
FormularioPolvo
Tipo de productoEstreptomicina
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C
Condiciones de envío: Ambiente
Vida útil: 24 meses a partir de la fecha de fabricación

Preguntas frecuentes

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (5)

Citations & References
Abstract
The cyclin-dependent kinase inhibitor p21WAF1/Cip1 is an antiestrogen-regulated inhibitor of Cdk4 in human breast cancer cells.
Authors: Skildum Andrew J; Mukherjee Shibani; Conrad Susan E;
Journal:J Biol Chem
PubMed ID:11741909
'The MCF-7 cell line is a model of estrogen-dependent, antiestrogen-sensitive human breast cancer. Antiestrogen treatment of MCF-7 cells causes dramatic decreases in both Cdk4 and Cdk2 activities, which leads to a G(1) phase cell cycle arrest. In this report, we investigate the mechanism(s) by which Cdk4 activity is regulated in ... More
Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.
Authors: Lund R D; Adamson P; Sauvé Y; Keegan D J; Girman S V; Wang S; Winton H; Kanuga N; Kwan A S; Beauchène L; Zerbib A; Hetherington L; Couraud P O; Coffey P; Greenwood J;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11504951
'Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RPE cell lines each exhibiting genetic modifications that confer long-term ... More
Exercise-induced a-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation.
Authors:Yuan Y, Xu P, Jiang Q, Cai X, Wang T, Peng W, Sun J, Zhu C, Zhang C, Yue D, He Z, Yang J, Zeng Y, Du M, Zhang F, Ibrahimi L, Schaul S, Jiang Y, Wang J, Sun J, Wang Q, Liu L, Wang S, Wang L, Zhu X, Gao P, Xi Q, Yin C, Li F, Xu G, Zhang Y, Shu G
Journal:EMBO J
PubMed ID:32104923
'Beneficial effects of resistance exercise on metabolic health and particularly muscle hypertrophy and fat loss are well established, but the underlying chemical and physiological mechanisms are not fully understood. Here, we identified a myometabolite-mediated metabolic pathway that is essential for the beneficial metabolic effects of resistance exercise in mice. We ... More
Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis.
Authors:Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM
Journal:EMBO J
PubMed ID:31268602
'The guanylate binding protein (GBP) family of interferon-inducible GTPases promotes antimicrobial immunity and cell death. During bacterial infection, multiple mouse Gbps, human GBP2, and GBP5 support the activation of caspase-1-containing inflammasome complexes or caspase-4 which trigger pyroptosis. Whether GBPs regulate other forms of cell death is not known. The apicomplexan ... More
Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA Coprotease.
Authors:Wipperman MF, Heaton BE, Nautiyal A, Adefisayo O, Evans H, Gupta R, van Ditmarsch D, Soni R, Hendrickson R, Johnson J, Krogan N, Glickman MS
Journal:Mol Cell
PubMed ID:30174294
'Infection with Mycobacterium tuberculosis continues to cause substantial human mortality, in part because of the emergence of antimicrobial resistance. Antimicrobial resistance in tuberculosis is solely the result of chromosomal mutations that modify drug activators or targets, yet the mechanisms controlling the mycobacterial DNA-damage response (DDR) remain incompletely defined. Here, we ... More