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MEM α, nucleosides, powder
MEM α, nucleosides, powder
MEM α, nucleosides, powder
MEM α, nucleosides, powder
Gibco™

MEM α, nucleosides, powder

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negativeWeitere Informationen
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KatalognummerMenge
1190007310 l
Katalognummer 11900073
Preis (EUR)
72,50
Each
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Menge:
10 l
Customize this product
Preis (EUR)
72,50
Each
Zum Warenkorb hinzufügen

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With: Ribonucleosides, Deoxyribonucleosides, L-glutamine, Phenol Red

Without: Sodium Bicarbonate

The complete formulation is available.

Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle's salts.

Product Intended Use

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Nicht geeignet für therapeutische Zwecke bei Menschen und Tieren. Zweckentfremdete Verwendung kann eine Verletzung lokaler gesetzlicher Bestimmungen darstellen. Zur Verwendung in der In-vitro-Diagnostik.
Specifications
ZelllinieHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7 und Fibroblasten
ZelltypPrimäre Ratten-Astrozyten
Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard
ProduktlinieGibco
ProdukttypMEM α (Minimum Essential Medium α)
Menge10 l
Haltbarkeit36 Monate ab Herstellungsdatum
VersandbedingungRaumtemperatur
KlassifikationOhne Stoffe tierischen Ursprungs
FormPulver
Serum LevelStandard-Serumzugabe
SterilitätSteril gefiltert
Mit AdditivenGeringe Glukosekonzentration, Glutamin, Phenolrot, Natriumpyruvat, Ribonukleoside, Desoxyribonukleoside
Ohne AdditiveKeine HEPES, Kein Natriumhydrogencarbonat
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen:2–8 °C
Versandbedingungen:Haltbarkeit bei
Raumtemperatur:36 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM α, nucleosides, powder, 10 L FAQs