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Gibco™

DMEM, powder, high glucose

Catalog number:  12100046

Related applications:

Mammalian Cell Culture

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Description

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• HEPES
• Phenol Red• Sodium Bicarbonate

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® DMEM product made in our Grand Island facility (12100-061). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Glucose: High Glucose
HEPES Buffer: No HEPES
Product Line: Gibco®
Quantity: 10 x 1L
Serum Supplementation: Standard Serum Supplementation
Shipping Condition: Room Temperature
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Sodium Pyruvate Additive: No Sodium Pyruvate
Form: Powder
Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red

Contents & storage

Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture