Insertos de cultivo celular de policarbonato Nunc™ en placas multipocillos
Insertos de cultivo celular de policarbonato Nunc™ en placas multipocillos
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Insertos de cultivo celular de policarbonato Nunc™ en placas multipocillos
Insertos de cultivo celular de policarbonato Nunc™ en placas multipocillos
Thermo Scientific™

Insertos de cultivo celular de policarbonato Nunc™ en placas multipocillos

Cultive la mayoría de los tipos de células sin revestimiento de matriz al emplear insertos de membrana de policarbonato en multiplacas Thermo Scientific™ Nunc™.
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Número de catálogoTamaño de poroTipo de placaÁrea de cultivo
1406298 μmPlaca de 24 pocillos0,47cm2
1406200,4 μmPlaca de 24 pocillos0,47cm2
1406273 μmPlaca de 24 pocillos0,47cm2
1406400,4 μmPlaca de 6 pocillos3,14 cm2
1406423 μmPlaca de 6 pocillos3,14 cm2
1406448 μmPlaca de 6 pocillos3,14 cm2
1406520,4 μmPlaca de 12 pocillos1,13cm2
1406543 μmPlaca de 12 pocillos1,13cm2
1406568 μmPlaca de 12 pocillos1,13cm2
1406600,4 μmPlaca de 6 pocillos4,1cm2
1406633 μmPlaca de 6 pocillos4,1cm2
1406688 μmPlaca de 6 pocillos4,1cm2
Número de catálogo 140629
Precio (MXN)
-
Tamaño de poro:
8 μm
Tipo de placa:
Placa de 24 pocillos
Área de cultivo:
0,47cm2
Cultive la mayoría de los tipos de células sin revestimiento de matriz al emplear insertos de membrana de policarbonato en multiplacas Thermo Scientific™ Nunc™.
Características:
  • Membrana porosa tratada para cultivo de tejidos para una fijación celular optimizada
  • Tres tamaños de poro: 0,4, 3 y 8 micrómetros
  • Preempaquetados en placas multipocillos estériles tratadas con Nunclon™ Delta
  • No tóxicos y no pirógenos

Aplicaciones:

  • Ensayos de invasión y migración de tumores
  • Ensayos de barreras epiteliales (p. ej., Caco-2) y endoteliales (p. ej., células endoteliales microvasculares cerebrales)
  • Modelos de tejidos dérmicos/epidérmicos y epiteliales (p. ej., modelo de piel artificial)
  • Estudios de quimiotaxia
  • Cocultivo

Garantía: 90 días

 

Exención de responsabilidad de la restricción de uso para investigación interna: cualquier restricción impuesta al comprador para utilizar el producto o productos únicamente para fines de investigación interna no se aplica al producto o productos que aparecen en las páginas web asociadas a esta declaración.

Referencias generales:

  1. Mardi A Crane-Godreau et. al (2005). CCL20/macrophage Inflammatory Protein 3alpha and Tumor Necrosis Factor Alpha Production by Primary Uterine Epithelial Cells in Response to Treatment With Lipopolysaccharide or Pam3Cys PMID: 15618187
  2. Rettinger CL et. al (2018). Quantitative Assessment of Retina Explant Viability in a Porcine Ex Vivo Neuroretina Model PMID: 29924674
  3. Mardi A Crane-Godreau et. al (2004). Effect of Escherichia coli and Lactobacillus rhamnosus on macrophage inflammatory protein 3 alpha, tumor necrosis factor alpha, and transforming growth factor beta release by polarized rat uterine epithelial cells in culture. PMID: 15039305
  4. Soboll G et. al (2006). Effect of oestradiol on PAMP-mediated CCL20/MIP-3 alpha production by mouse uterine epithelial cells in culture PMID: 16771853
  5. Mardi A Crane-Godreau et. al (2009). Exposure to Cigarette Smoke Disrupts CCL20-Mediated Antimicrobial Activity in Respiratory Epithelial Cells PMID: 19966927
  6. Wildemann B et. al (2005). Differences in the fusion and resorption activity of human osteoclasts after stimulation with different growth factors released from a polylactide carrier PMID: 15477999
  7. Sugita Y et. al (1997). Pigment epithelium-derived factor (PEDF) has direct on the metabolism and proliferation of microglia and indirect effects on astrocytes PMID: 9335258
  8. van Schilfgaarde M et. al (1999). Haemophilus influenzae localized in epithelial cell layers is shielded from antibiotics and antibody-mediated bactericidal activity PMID: 10222210
  9. Chung HC et. al (2014). Inhibition of porcine endogenous retrovirus in PK15 cell line by efficient multitargeting RNA interference PMID: 24138389
  10. Ode A et. al (2010). Toward biomimetic materials in bone regeneration: functional behavior of mesenchymal stem cells on a broad spectrum of extracellular matrix components PMID: 20878902
Especificaciones
ColorTransparente
DescripciónInsertos de cultivo celular de policarbonato con tamaño de poro de 8 micras en placa de 24 pocillos
Tipo de placaPlaca de 24 pocillos
N.º por caja48
N.° por paquete12
Tratamiento de superficieTratamiento para cultivo celular
Área de cultivo0,47cm2
Para utilizar conMultiplaca de 24 pocillos
Insertos por placa12
MaterialPolicarbonato (membrana)
N.° de pocillos24
Densidad de los poros<0,85 x 10^5
Tamaño de poro8 μm
EsterilidadEstériles
TipoInserto de cultivo
Volumen de trabajo (métrico)0,5 ml
Unit SizeCase of 48

Preguntas frecuentes

What is the difference between the 24-well plate format for the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620) and the 24-well plate format for the Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002)?

The difference between the two 24-well plate formats is as follows:

  • Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620): There are only 12 inserts in the 24-well multi-well plate, leaving every second column free without an insert. This is a preferred configuration for some co-culture experiments, as it makes it easy to access the culture grown in the multi-well plate for media changes, etc., by placing the insert in the empty well next to it. The inserts are placed directly into the wells here, leaving a 0.9 mm gap between the bottom of the well and the insert membrane.
  • Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002): There are 24 inserts in the 24-well carrier plate. The carrier plate with the hanging slots allows for adjustment of the insert height. The three hanging positions in the carrier plate allow for distance of approximately 0.9 mm, 3.3 mm, or 6.3 mm between the well-bottom and insert growth surface for both 12- and 24-well plate formats. This configuration is preferred when customer needs more versatility, for example to facilitate air-liquid interface culture, and other co-culture applications.


Note: details on plate configuration can also be found within the following product brochure (pg 3).

With the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates, what is the distance between the cell culture membrane and the bottom of the multi-well dish?

The membrane is approximately 1.0 mm above the bottom of the dish for the Nunc Polycarbonate Cell Culture Inserts.

Can I observe cells on the cell culture membrane when the Nunc Polycarbonate Cell Culture Insert is inside of a multi-well plate?

Due to the large pore size of the Nunc polycarbonate inserts, spots or holes are observed and they represent the membrane. In this plane of focus, the most visible cells are the ones located on the membrane, whereas the slightly blurred backdrop are the cells on the dish that are in a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of the membrane. However, these cells are visible in culture.

What is the recommended Multidish chamber media volume per carrier plate insert and height position?

Low position: 2 mL (12-well), 1 mL (24-well), Medium position: 3 mL (12-well), 1.5 mL (24-well), High position: 4 mL (12-well), 2 mL (24-well). Some optimization may be necessary for development purposes.

Why are only 12 inserts provided with standard membrane insert products in the 24-well format?

This has emerged as the industry standard. In many cases having empty wells facilitates media changes and other experimental steps.

View all Insertos de cultivo celular de policarbonato Nunc™ en placas multipocillos FAQs