ProSwift™ RP-4H Monolithic Phenyl (Ph) Reversed Phase HPLC Column, Monolith, 0.2 mm x 250 mm

Run protein separations at exceptionally high flow rates with Thermo Scientific™ ProSwift™ RP LC Columns. These reversed-phase monolith columns deliver the outstanding resolving power of nonporous analytical media combined with faster separations and analysis than other bead-based columns. ProSwift polymer monolith columns achieve long column lifetime, exhibit reproducibility even after hundreds of runs and have broad pH stability, providing high stringent wash capability (1M NaOH).

Each monolith is a single cylindrical polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size. These large channels and the monolith's nonporous surfaces provide fast mass transfer for the high-resolution and fast protein separations. The monolith morphology results in low operating backpressure, allowing the use of higher linear velocities with minimal loss of resolution.

Advantages of High Resolution at Exceptionally High Flow Rates

• Highest operational flow rates available
• High throughput and improved productivity
• Excellent stability over a wide pH range of 1 to 14
• Outstanding reproducibility and ruggedness
• High loading capacity

Fast, Sensitive Protein Separations

• High speed protein separations
• High sensitivity for LC-MS
• High column-to-column reproducibility
• Wide range of column I.D. and lengths available
• Superior lifetime

Easy Column Installation

• Use Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fittings

Preconcentrate and Desalt Samples   

• Precolumns are available to preconcentrate and desalt samples.
• You can also use Thermo Scientific™ PepSwift™ Trap Columns for preconcentration and desalting of samples consisting of peptides and proteins without negatively impacting the chromatographic performance or recovery of the compounds.
• Change the selectivity of the separation or improve the trapping efficiency by employing various ion-pairing agents in the loading solvent and/or mobile phases.