Invitrogen™

DNA Polymerase I

Catalog number:  18010017

Related applications:

Restriction Enzyme Cloning

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Description

DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.

Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs
Source—purified from E. coli expressing the DNA Polymerase I gene on a plasmid

Performance and quality testing

Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

Unit definition

One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.

Unit reaction conditions

50 mM potassium phosphate (pH 7.0), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 µg/mL template/primer, 32 µM dTTP, 69 nM [3H]dTTP, and enzyme in 100 µL for 30 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity: 3' - 5', 5' - 3'
Hot Start: No
Polymerase: DNA Polymerase I
Shipping Condition: Wet Ice
Quantity: 250 units

Contents & storage

Store in freezer (-5 to -30°C).