Invitrogen™

DNA Polymerase I, Large (Klenow) Fragment

Catalog number: 18012039
Invitrogen™

DNA Polymerase I, Large (Klenow) Fragment

Catalog number: 18012039
Catalog Number
18012039
also known as 18012-039
Unit Size
500 units
Price (USD)
598.00
Your Price:
Availability
***
Quantity
Catalog Number
18012021
also known as 18012-021
Unit Size
100 units
Price (USD)
189.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
18012039
also known as 18012-039
500 units
598.00
Your Price:
***
18012021
also known as 18012-021
100 units
189.00
Your Price:
***
Product Overview
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DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.

Applications:
Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Specifications

Exonuclease Activity
3' - 5'
Hot Start
No
Polymerase
DNA Polymerase I
Shipping Condition
Approved for shipment on Wet or Dry Ice
Product Type
DNA Polymerase
Quantity
500 units

Contents & Storage

DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM
MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C.

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