Invitrogen™

DNA Polymerase I, Large (Klenow) Fragment

Catalog number:  18012039

Related applications:

Genotyping & Genomic Profiling

| Restriction Enzyme Cloning

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Description

DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.

Applications:
Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity: 3' - 5'
Hot Start: No
Polymerase: DNA Polymerase I
Shipping Condition: Approved for shipment on Wet or Dry Ice
Quantity: 500 units

Contents & storage

DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact® 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM
MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C.