Invitrogen™

T7 RNA Polymerase

Catalog number: 18033019
Invitrogen™

T7 RNA Polymerase

Catalog number: 18033019
Catalog Number
18033019
Unit Size
2500 units
Price (USD)
Price: 340.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
2 x 2,500 units
Price (USD)
Price: 552.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
180330192500 units
Price: 340.00
Your Price:
-
180331002 x 2,500 units
Price: 552.00
Your Price:
-
Product Overview
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Additional Information
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T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.

Application:
Synthesis of labeled and unlabeled RNA transcripts (1).

Source:
Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid.

Performance and Quality Testing:
3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a
transcription reaction.

Unit Definition:
One unit hydrolyzes 1 nmol of ribonucleotide into acid-precipitable material in 1 h at 37°C using a T7 transcription
vector as template.

Unit Reaction Conditions:
40 mM Tris-HCl (pH 8.0), 25 mM NaCl, 8 mM MgCl2 , 2 mM spermidine-(HCl)3 , 5 mM DTT, 0.4 mM ATP, 0.4 mM CTP, 0.4 mM GTP, 0.4 mM UTP, 1 µCi [3H]GTP, 1 µg Sph I-cut pT7L13, and enzyme in 50 µl for 10 min. at 37°C.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
DNA Polymerase
Assay
DNA Nicking Assay
Hot Start
No
Polymerase
T7 RNA Polymerase
Shipping Condition
Wet Ice
Promoter
T7
Quantity
2500 U

Contents & Storage

T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2 , 10 mM spermidine-(HCl)3 , 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.