Invitrogen™

SuperScript™ III First-Strand Synthesis System

Catalog number: 18080051
Invitrogen™

SuperScript™ III First-Strand Synthesis System

Catalog number: 18080051
Catalog Number
18080051
also known as 18080-051
Unit Size
50 reactions
Price (USD)
496.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
18080051
also known as 18080-051
50 reactions
496.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
The SuperScript® III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg–5 µg of total RNA. SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first-strand cDNA from a total RNA preparation.

Using the SuperScript® III First-Strand System
cDNA synthesis is performed in the first step using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer. In the second step, PCR is performed in a separate tube using primers specific for the gene of interest. For the PCR reaction, we recommend one of the following DNA polymerases: Platinum® Taq DNA Polymerase provides automatic hot-start conditions for increased specificity up to 4 kb, Platinum® Taq DNA Polymerase High Fidelity provides increased yield and high fidelity for targets up to 15 kb, and Platinum® Pfx DNA Polymerase provides maximum fidelity for targets up to 12 kb.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR)
No. of Tests
50 Reactions
Optimal Reaction Temperature
50° C
Product Line
SuperScript™
Product Type
First-Strand Synthesis System
Reverse Transcriptase
SuperScript™ III
Shipping Condition
Dry Ice
Sufficient For
50 Reactions
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
50 Reactions
PCR Method
RT-PCR
Reaction Format
Separate Components
Volume
20 μL

Contents & Storage

The SuperScript™ III First-Strand Synthesis System for RT-PCR is supplied with the following:
• Oligo(dT)20 (50 µM), 50 µl
• Random hexamers (50 ng/µl), 250 µl
• 10X RT buffer, 1 mL
• 0.1 M DTT, 250 µl
• 25 mM magnesium chloride, 500 µl
• 10 mM dNTP mix, 250 µl
• SuperScript III RT (200 U/µl), 50 µl
• RNaseOUT (40 U/µl), 100 µl
E. coli RNase H (2 U/µl), 50 µl
• DEPC-treated water, 1.2 mL
• Total HeLa RNA (10 ng/µl), 20 µl
• Sense Control Primer (10 µM), 25 µl
• Antisense Control Primer (10 µM), 25 µl

Sufficient for 50 reactions, based on a 20 µl reaction size. Store at -20°C.

Figures

Frequently asked questions (FAQs)

Citations & References