Can exo-Klenow be purchased separately from your array labeling kits?
Yes, the catalog number for the exo-Klenow is AM2008. The enzyme concentration is 5 U/µL.
引用および参考文献 (10)
引用および参考文献
Abstract
A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization.
Authors:Chung YJ, Jonkers J, Kitson H, Fiegler H, Humphray S, Scott C, Hunt S, Yu Y, Nishijima I, Velds A, Holstege H, Carter N, Bradley A,
Journal:Genome Res
PubMed ID:14707179
'Microarray-based comparative genomic hybridization (CGH) has become a powerful method for the genome-wide detection of chromosomal imbalances. Although BAC microarrays have been used for mouse CGH studies, the resolving power of these analyses was limited because high-density whole-genome mouse BAC microarrays were not available. We therefore developed a mouse BAC ... More
DNA replication origins fire stochastically in fission yeast.
Authors:Patel PK, Arcangioli B, Baker SP, Bensimon A, Rhind N,
Journal:Mol Biol Cell
PubMed ID:16251353
'DNA replication initiates at discrete origins along eukaryotic chromosomes. However, in most organisms, origin firing is not efficient; a specific origin will fire in some but not all cell cycles. This observation raises the question of how individual origins are selected to fire and whether origin firing is globally coordinated ... More
A virulence and antimicrobial resistance DNA microarray detects a high frequency of virulence genes in Escherichia coli isolates from Great Lakes recreational waters.
Authors:Hamelin K, Bruant G, El-Shaarawi A, Hill S, Edge TA, Bekal S, Fairbrother JM, Harel J, Maynard C, Masson L, Brousseau R,
Journal:Appl Environ Microbiol
PubMed ID:16751532
'Escherichia coli is generally described as a commensal species with occasional pathogenic strains. Due to technological limitations, there is currently little information concerning the prevalence of pathogenic E. coli strains in the environment. For the first time, using a DNA microarray capable of detecting all currently described virulence genes and ... More
Microarray deacetylation maps determine genome-wide functions for yeast histone deacetylases.
'Yeast contains a family of five related histone deacetylases (HDACs) whose functions are known at few genes. Therefore, we used chromatin immunoprecipitation and intergenic microarrays to generate genome-wide HDAC enzyme activity maps. Rpd3 and Hda1 deacetylate mainly distinct promoters and gene classes where they are recruited largely by novel mechanisms. ... More
Characterization of the genome composition of Bartonella koehlerae by microarray comparative genomic hybridization profiling.
Authors:Lindroos HL, Mira A, Repsilber D, Vinnere O, Näslund K, Dehio M, Dehio C, Andersson SG,
Journal:J Bacteriol
PubMed ID:16109957
Bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. We have estimated here the gene content of Bartonella koehlerae, a novel species isolated from cats that was recently identified as an agent of human endocarditis. The ... More