The BioPrime™ DNA Labeling System is specifically designed for use in the preparation of biotinylated probes. Random primers (octamers) areRead more
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Catalog number 18094011
Price (USD)/ Each
643.65
Online exclusive
704.00
Save 60.35 (9%)
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Add to cart
Price (USD)/ Each
643.65
Online exclusive
704.00
Save 60.35 (9%)
Add to cart
BioPrime™ DNA Labeling System
Catalog number18094011
Price (USD)/ Each
643.65
Online exclusive
704.00
Save 60.35 (9%)
-
Add to cart
The BioPrime™ DNA Labeling System is specifically designed for use in the preparation of biotinylated probes. Random primers (octamers) are annealed to the denatured DNA template and extended by Klenow fragment in the presence of biotin-14-dCTP to produce sensitive biotinylated-DNA probes for use in the nonradioactive detection of DNA and RNA. With the BioPrime™ DNA Labeling System, considerable net DNA synthesis occurs resulting in a 10–40 fold amplification of the probe. This product is particularly well suited for situations in which the DNA is limited. Probes made with this product have been used for hybridization to Southern and northern blots, plaque and colony lifts, and for in situ hybridization to chromosome spreads. The BioPrime™ DNA Labeling System:
• Requires less template DNA than nick translation • Amplifies template to provide an increased amount of biotinylated probe • Labels 50 to 500 ng of template DNA in one reaction
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodBiotin-based
Label or DyeBiotin
Final Product TypeProbes (Labeled DNA)
Labeling TargetDNA (General), Genomic DNA
Includes Label or DyeYes
Labeling MethodDirect Labeling
Product LineBioPrime™
Product TypeDNA Labeling System
Quantity30 Reactions
Shipping ConditionApproved for shipment on Wet or Dry Ice
Unit SizeEach
Contents & Storage
• 700 μl 2.5X Random Primers Solution • 175 μl 10X dNTP Mixture • 100 μg Control DNA • 35 μl Klenow Fragment (40 U/μl) • 500 μl Stop Buffer • 1.25 ml Distilled Water