BioPrime® DNA Labeling System

Catalog number: 18094011

Invitrogen™  Related applications: Genotyping & Genomic Profiling | Nucleic Acid Gel Electrophoresis & Blotting

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Description

The BioPrime® DNA Labeling System is specifically designed for use in the preparation of biotinylated probes. Random primers (octamers) are annealed to the denatured DNA template and extended by Klenow fragment in the presence of biotin-14-dCTP to produce sensitive biotinylated-DNA probes for use in the nonradioactive detection of DNA and RNA. With the BioPrime® DNA Labeling System, considerable net DNA synthesis occurs resulting in a 10–40 fold amplification of the probe. This product is particularly well suited for situations in which the DNA is limited. Probes made with this product have been used for hybridization to Southern and northern blots, plaque and colony lifts, and for in situ hybridization to chromosome spreads. The BioPrime® DNA Labeling System:

• Requires less template DNA than nick translation
• Amplifies template to provide an increased amount of biotinylated probe
• Labels 50 to 500 ng of template DNA in one reaction
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Biotin-Based
Final Product: Probes (Labeled DNA)
Includes Label or Dye: Yes
Label or Dye: Biotin
Labeling Method: Direct Labeling
Labeling Target: DNA (General), Genomic DNA
Product Size: 30 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

• 700 µl 2.5X Random Primers Solution
• 175 µl 10X dNTP Mixture
• 100 µg Control DNA
• 35 µl Klenow Fragment (40 U/µl)
• 500 µl Stop Buffer
• 1.25 ml Distilled Water

Store at -20°C in a non-frost-free freezer.

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