CaptureSelect™ IgG-CH1 Affinity Matrix

Catalog number: 19432005L

 Related applications: Antibody Purification | Protein Purification & Isolation

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Description

CaptureSelect™ IgG-CH1 Affinity Matrix has been specifically designed for the purification of recombinant human Fab fragments and IgGs, recognizing all IgG subclasses (1, 2, 3 and 4) independent of the light chain subclass (Kappa/Lambda).

Features of this affinity matrix include:

• Platform approach for purification of IgGs and their Fab fragments
• No co-purification of overexpressed free lights chains due to heavy chain binding
• Excellent scalability
• Non-animal-derived

CaptureSelect™ IgG-CH1 Affinity Matrix purifies IgG, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.

Free of Animal Components
Our CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single domain antibody fragments. The ligand is a 14 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ IgG-CH1 affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 25–30 g IgG/L resin depending on flow rate, column height and contact time
Elution conditions: 20 mM citric acid, 150 mM NaCl, pH 3.5
Flow characteristics: 200–400 cm/h
Formulation buffer: 20%(v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Bottle(s)
Flow Rate: 200 to 400 cm⁄hr
Column Type: Bulk Resin
Product Size: 5 L
Particle Size: 65 micron
Support Matrix: Agarose based, aldehyde activated
Immobilized Phase: Anti IgG-CH1

Contents & storage

Store in refrigerator (2–8°C).

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Manuals & protocols

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