Limited time offer: 3 year warranty when you purchase a SimpliAmp Thermal Cycler. View flyer »

Carboxypeptidase Y

Catalog number: 20212

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Thermo Scientific Pierce Carboxypeptidase Y is a protease with that is able to cleave (release) any terminal amino acid including proline from the C-terminus of peptides.

Carboxypeptidase Y is a glycoprotein exopeptidase of the serine class. Carboxypeptidase Y has a broad amino acid specificity, and is able to release every amino acid including proline from the carboxyl-terminus of peptides, although glycine and aspartic acid are released much more slowly than other residues. It is active in urea and SDS solutions and retains its activity under the denaturing conditions used for polypeptide sequencing.

Properties of Carboxypeptidase Y
• Alternative names: Proteinase C, yeast carboxypeptidase Y
• Specificity: Cleaves residues sequentially from the carboxy terminus
• Source: S. cerevisiae (bakers yeast)
• Molecular weight: 61,000
• Protease type: Serine exopeptidase
• Uses/applications: Peptide hydrolysis for sequencing
• Reaction conditions: pH 5.5-7.5, 25°C
• Inhibitors: PMSF and DFP
• Unit definition: One unit of carboxypeptidase Y hydrolyzes one µMol of benzyl-oxycarbonyl-L-phenylalanyl-L-leucine per minute at 25°C, pH 6.5

Immobilized carboxypeptidase Y can be substituted for free carboxypeptidase Y in any application, and is advantageous because it virtually eliminates autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the carboxypeptidase Y. Immobilized carboxypeptidase Y is also more stable against heat-induced denaturation, resulting in longer maintenance of activity.

Related Products
Immobilized Carboxypeptidase Y (Agarose Resin)
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 1 mg

Contents & storage

Store at -20°C.


Manuals & protocols

Recommended products