Search

  • Order Status
  • Quick Order
  • Promos

Proteintech

NR1H4 Polyclonal Antibody

39 References
View all (19) FXR antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
NR1H4 Polyclonal Antibody

Product Details

25055-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 28 publications 28 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunocytochemistry (ICC/IF)

Assay-dependent
View 8 publications 8 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NR1H4 Fusion Protein Ag21878 (1-121 aa encoded by BC130573)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Amount

40.5 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGSKMNLIE HSHLPTTDEF SFSENLFGVL TEQVAGPLGQ NLEVEPYSQY SNVQFPQVQP QISSSSYYSN LGFYPQQPEE WYSPGIYELR RMPAETLYQG ETEVAEMPVT KKPRMGASAG RI (1-121 aa encoded by BC130573)

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Bile acid receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; nuclear receptor; Nuclear receptor subfamily 1 group H member 4; putative new variant; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14; unnamed protein product

View more View less

Gene Aliases: BAR; FXR; HRR-1; HRR1; NR1H4; PFIC5; RIP14

View more View less

UniProt ID: (Human) Q96RI1

View more View less

Entrez Gene ID: (Human) 9971

View more View less

Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding zinc ion binding ligand-dependent nuclear receptor binding bile acid binding lithocholic acid receptor activity sequence-specific DNA binding metal ion binding retinoid X receptor binding chenodeoxycholic acid binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cellular glucose homeostasis immune system process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response cell-cell junction assembly Notch signaling pathway bile acid metabolic process negative regulation of macromolecule biosynthetic process negative regulation of tumor necrosis factor-mediated signaling pathway negative regulation of very-low-density lipoprotein particle remodeling regulation of low-density lipoprotein particle clearance bile acid and bile salt transport cell differentiation intracellular receptor signaling pathway negative regulation of interferon-gamma production negative regulation of interleukin-1 production negative regulation of interleukin-2 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-17 production toll-like receptor 9 signaling pathway regulation of urea metabolic process cellular triglyceride homeostasis positive regulation of insulin secretion involved in cellular response to glucose stimulus intracellular bile acid receptor signaling pathway glucose homeostasis cholesterol homeostasis defense response to bacterium negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of insulin receptor signaling pathway negative regulation of inflammatory response fatty acid homeostasis regulation of insulin secretion involved in cellular response to glucose stimulus triglyceride homeostasis regulation of bile acid biosynthetic process cellular response to lipopolysaccharide cellular response to fatty acid negative regulation of monocyte chemotactic protein-1 production regulation of cholesterol metabolic process cellular response to bile acid positive regulation of adipose tissue development positive regulation of phosphatidic acid biosynthetic process positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support