Invitrogen™

Proteinase K, recombinant

Catalog number: 25530015
Invitrogen™

Proteinase K, recombinant

Catalog number: 25530015
Catalog Number
25530015
Unit Size
100 mg
Price (USD)
Price: 158.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
1 g
Price (USD)
Price: 1,152.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
25530015100 mg
Price: 158.00
Your Price:
-
255300311 g
Price: 1,152.00
Your Price:
-
Product Overview
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Additional Information
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Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Powder
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice
Quantity
100 mg
Product Type
Proteinase K

Contents & Storage

One vial

Store in refrigerator (2–8°C).