This product is manufactured in our Paisley, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Grand Island, US, is recommended for our customers in North America, Latin America, and the Asia/Pacific region. Click to view.
Gibco™

Medium 199, Earle's Salts This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  31150022

Related applications:

Mammalian Cell Culture

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Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

WithWithout
• L-glutamine• HEPES
• Phenol Red

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® Medium 199 product made in our Grand Island facility (11150-059). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Classification: Animal Origin
Concentrated: 1 X
Culture Environment: CO2
Form: Liquid
Glutamine: L-Glutamine
Green Features: Sustainable packaging
HEPES Buffer: No HEPES
Osmolality: 290 - 310 mOsm/kg
Phenol Red Indicator: Phenol Red
Product Line: Gibco®
Product Size: 500 mL
Shipping Condition: Room Temperature
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: No Sodium Pyruvate
Supplementation Required: Serum
Volume (Metric): 500 ml
pH Range: 7.1 - 7.4

Contents & storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Media formulations