Informational:This product is manufactured in our Paisley, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Grand Island, US, is recommended for our customers in North America, Latin America, and the Asia/Pacific region. Click to view.
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Gibco™

Medium 199, Earle's Salts

Catalog number: 31150022
Have Questions?
Gibco™

Medium 199, Earle's Salts

Catalog number: 31150022
Have Questions?
Catalog Number
31150022
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
-
Catalog Number
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
10 x 500 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
31150022500 mL
--
11150059500 mL
-
1115006710 x 500 mL
-
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Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This Medium 199 is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red 


The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Culture Environment
CO2
Cell Type
Mammalian Cells
Classification
Animal Origin
Concentration
1X
Culture Type
Mammalian Cell Culture
Form
Liquid
Product Type
Medium 199
Shelf Life
12 Months
Sterility
Sterile-filtered
With Additives
Glutamine, Phenol Red, Earle's Balanced Salt Solution (EBSS)
Without Additives
No HEPES, No Sodium Pyruvate, No Sodium Pyruvate, No Sodium Bicarbonate
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Osmolality
290 - 310 mOsm/kg
pH
7.1 to 7.4
Product Line
Gibco™
Quantity
500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2–8°C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

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