Detection of differentially expressed glycogenes in trabecular meshwork of eyes with primary open-angle glaucoma.
AuthorsDiskin S; Kumar J; Cao Z; Schuman JS; Gilmartin T; Head SR; Panjwani N
JournalInvestigative Ophthalmology & Visual Science
PubMed ID
PURPOSE: To identify differentially expressed glycogenes in trabecular meshwork (TM) of eyes with primary open-angle glaucoma (POAG). METHODS: Total RNA was isolated from TM of cadaveric eyes derived from donors with diagnosed glaucomas of different etiologies and from normal control subjects. RNA was amplified and hybridized to the GLYCOv2 oligonucleotide ... More
Attenuated Coxiella burnetii phase II causes a febrile response in gamma interferon knockout and Toll-like receptor 2 knockout mice and protects against reinfection.
Coxiella burnetii is a highly infectious obligate intracellular bacterium. The phase I form is responsible for Q fever, a febrile illness with flu-like symptoms that often goes undiagnosed. The attenuated C. burnetii phase II (having a truncated ""O"" chain of its lipopolysaccharide) does not cause disease in immunocompetent animals; however, ... More
Angiogenic effects of human multipotent stromal cell conditioned medium activate the PI3K-Akt pathway in hypoxic endothelial cells to inhibit apoptosis, increase survival, and stimulate angiogenesis.
AuthorsHung SC; Pochampally RR; Chen SC; Hsu SC; Prockop DJ
JournalStem Cells (Dayton, Ohio)
PubMed ID
Recent reports indicated that vascular remodeling and angiogenesis are promoted by conditioned medium from the cells referred to as multipotent stromal cells (MSCs). However, the molecular events triggered by MSC-conditioned medium (CdM) were not defined. We examined the effects of CdM from human MSCs on cultures of primary human aortic ... More
Molecular characterization of selected dermatophytes and their identification by electrophoretic mutation scanning
Dermatophytes are fungi that can be contagious and cause infections in the keratinized skin of mammals, including humans. The etiological diagnosis of dermatophytosis relies on a combination of in vitro-culture and microscopic methods. Effective molecular tools could overcome the limitations of conventional methods of identification. In the present study, following ... More
Molecular Population Genetics of the SRK and SCR Self-Incompatibility Genes in the Wild Plant Species Brassica cretica (Brassicaceae)
AuthorsEdh, K; Widen, B; Ceplitis, A
JournalGenetics
PubMed ID
Self-incompatibility (SI) in plants is a classic example of a trait evolving under strong frequency-dependent selection. As a consequence, population genetic theory predicts that the S locus, which controls SI, should maintain numerous alleles, display a high level of nucleotide diversity, and, in structured populations, show a lower level of ... More
NGALR Is Overexpressed and Regulated by Hypomethylation in Esophageal Squamous Cell Carcinoma
AuthorsCui, L; Xu, LY; Shen, ZY; Tao, Q; Gao, SY; Lv, Z; Du, ZP; Fang, WK; Li, EM
JournalCLINICAL CANCER RESEARCH
PubMed ID
Purpose: Neutrophil gelatinase-associated lipocalin receptor (NGALR) m RNA level is reduced in isolated chronic myelogenous leukemia blasts but up-regulated in esophageal squamous cell carcinoma (ESCC). The mechanism of NGALR regulation is unknown. Here, we show the expression pattern of NGALR and examine the aberrant methylation of its gene in ESCC ... More
The narQP genes for a two-component regulatory system from the deep-sea bacterium Shewanella violacea DSS12
AuthorsTamegai, H; Chikuma, S; Ishii, M; Nakasone, K; Kato, C
JournalMitochondrial DNA
PubMed ID
Shewanella violacea DSS12 is facultative piezophile isolated from the deep-sea. The expression of cydDC genes (required for d-type cytochrome maturation) of the organism is regulated by hydrostatic pressure. In this study, we analyzed the nucleotide sequence upstream of cydDC in detail and found that there are putative binding sites for ... More
Genetic polymorphisms affecting clinical outcomes in epithelial ovarian cancer patients treated with taxanes and platinum compounds: A Korean population-based study
AuthorsKim, HS; Kim, MK; Chung, HH; Kim, JW; Park, NH; Song, YS; Kang, SB
JournalGYNECOLOGIC ONCOLOGY
PubMed ID
Objective. We sought to evaluate the affect of genetic polymorphisms on clinical outcomes in patients with epithelial ovarian cancer (EOC). Methods. Clinical data of 118 patients between March 2001 and November 2006 were reviewed. They underwent staging laparotomy followed by adjuvant chemotherapy using taxanes and platinum compounds. We investigated ... More
Non-invasive genetic identification of small mammal species using real-time polymerase chain reaction
AuthorsMoran, S; Turner, PD; O'Reilly, C
JournalMolecular Ecology Resources
PubMed ID
DNA identification of non-invasive samples is a potentially useful tool for monitoring small mammal species. Here we describe a novel method for identifying five small mammal species: wood mouse, bank vole, common shrew, pygmy shrew and water shrew. Species-specific real-time polymerase chain reaction primers were designed to amplify fragments of ... More
Use of multiplex PCR and real-time PCR to detect human herpes virus genome in ocular fluids of patients with uveitis
AuthorsSugita, S; Shimizu, N; Watanabe, K; Mizukami, M; Morio, T; Sugamoto, Y; Mochizuki, M
JournalBRITISH JOURNAL OF OPHTHALMOLOGY
PubMed ID
Aim: To measure the genomic DNA of human herpes viruses (HHV) in the ocular fluids and to analyse the clinical relevance of HHV in uveitis. Methods: After informed consent was obtained, a total of 111 ocular fluid samples ( 68 aqueous humour and 43 vitreous fluid samples) were collected ... More
The narQP genes for a two-component regulatory system from the deep-sea bacterium Shewanella violacea DSS12
AuthorsTamegai, H; Chikuma, S; Ishi, M; Nakasone, K; Kato, C
JournalDNA SEQUENCE
PubMed ID
Shewanella violacea DSS12 is facultative piezophile isolated from the deep-sea. The expression of cydDC genes (required for d-type cytochrome maturation) of the organism is regulated by hydrostatic pressure. In this study, we analyzed the nucleotide sequence upstream of cydDC in detail and found that there are putative binding sites for ... More
Detection of eight GMO maize events by qualitative, multiplex PCR and fluorescence capillary gel electrophoresis
AuthorsHeide, BR; Heir, E; Holck, A
JournalEUROPEAN FOOD RESEARCH AND TECHNOLOGY
PubMed ID
Specific legislation in the EU and several other countries requires that foods containing genetically modified organisms (GMOs) should be approved and labelled. This has necessitated the development of methods for detection of such materials. For screening purposes these methods should preferably enable detection of several different GMOs. Here we present ... More
Significant association between TIM1 promoter polymorphisms and protection against cerebral malaria in thailand
Although cerebral malaria is a major life-threatening complication of Plasmodium falciparum infection, its pathophysiology is not well understood. Prolonged activation of the T helper type 1 (Th1) response characterized by the production of pro-inflammatory cytokines such as IFN-γ and TNF-α has been suggested to be responsible for immunopathological process leading ... More
In vitro assays fail to predict in vivo effects of regulatory polymorphisms.
AuthorsCirulli ET; Goldstein DB
JournalHuman Molecular Genetics
PubMed ID
A typical paradigm in the investigation of complex human disease is to assess the effects of cis-regulatory polymorphisms implicated in association studies on transcription in cellular expression systems. Evidence from in vitro transfection studies is often assumed to be sufficient evidence for the in vivo functional importance of a polymorphism ... More
Effects of 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 on cytokine production by human decidual cells.
The active form of vitamin D, 1,25-dihydroxyvitamin D(3) (1,25[OH](2)D(3)) is a potent immunomodulatory seco-steroid. We have demonstrated that several components of vitamin D metabolism and signaling are strongly expressed in human uterine decidua from first trimester pregnancies, suggesting that locally produced 1,25(OH)(2)D(3) may exert immunosuppressive effects during early stages of ... More
Nitric oxide mediates natural polyphenol-induced Bcl-2 down-regulation and activation of cell death in metastatic B16 melanoma.
AuthorsFerrer P; Asensi M; Priego S; Benlloch M; Mena S; Ortega A; Obrador E; Esteve JM; Estrela JM
JournalThe Journal of Biological Chemistry
PubMed ID
Intravenous administration to mice of trans-pterostilbene (t-PTER; 3,5-dimethoxy-4'-hydroxystilbene) and quercetin (QUER; 3,3',4',5,6-pentahydroxyflavone), two structurally related and naturally occurring small polyphenols, inhibits metastatic growth of highly malignant B16 melanoma F10 (B16M-F10) cells. t-PTER and QUER inhibit bcl-2 expression in metastatic cells, which sensitizes them to vascular endothelium-induced cytotoxicity. However, the molecular ... More
Rapid onset of gene expression in lung, supportive of formation of alveolar septa, induced by refeeding mice after calorie restriction.
JournalAmerican Journal of Physiology. Lung Cellular and Molecular Physiology
PubMed ID
Alveolar regenerative gene expression is unidentified partly because its onset, after a regenerative stimulus, is unknown. Toward addressing this void, we used a mouse model in which calorie restriction produces alveolar loss, and ad libitum access to food after calorie restriction induces alveolar regeneration. We selected four processes (cell replication, ... More
Trps1 plays a pivotal role downstream of Gdf5 signaling in promoting chondrogenesis and apoptosis of ATDC5 cells.
AuthorsItoh S; Kanno S; Gai Z; Suemoto H; Kawakatsu M; Tanishima H; Morimoto Y; Nishioka K; Hatamura I; Yoshida M; Muragaki Y
JournalGenes to Cells : Devoted to Molecular & Cellular Mechanisms
PubMed ID
Tricho-rhino-phalangeal syndrome (TRPS) is an autosomal dominant skeletal disorder caused by mutations of TRPS1. Based on the similar expression patterns of Trps1 and Gdf5, we hypothesized a possible functional interaction between these two molecules. Using a chondrogenic cell line (ATDC5), we investigated the association of Gdf5-mediated signaling pathways with Trps1 ... More
Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide.
AuthorsNocker A; Camper AK
JournalApplied and Environmental Microbiology
PubMed ID
The distinction between viable and dead bacterial cells poses a major challenge in microbial diagnostics. Due to the persistence of DNA in the environment after cells have lost viability, DNA-based quantification methods overestimate the number of viable cells in mixed populations or even lead to false-positive results in the absence ... More
JournalInvestigative Ophthalmology & Visual Science
PubMed ID
PURPOSE: The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in an animal model. METHODS: Retinal tissues from animals with ... More
Functional implications of calcium permeability of the channel formed by pannexin 1.
Although human pannexins (PanX) are homologous to gap junction molecules, their physiological function in vertebrates remains poorly understood. Our results demonstrate that overexpression of PanX1 results in the formation of Ca(2+)-permeable gap junction channels between adjacent cells, thus, allowing direct intercellular Ca(2+) diffusion and facilitating intercellular Ca(2+) wave propagation. More ... More
Cell- and gene-specific regulation of primary target genes by the androgen receptor.
AuthorsBolton EC; So AY; Chaivorapol C; Haqq CM; Li H; Yamamoto KR
JournalGenes & Development
PubMed ID
The androgen receptor (AR) mediates the physiologic and pathophysiologic effects of androgens including sexual differentiation, prostate development, and cancer progression by binding to genomic androgen response elements (AREs), which influence transcription of AR target genes. The composition and context of AREs differ between genes, thus enabling AR to confer multiple ... More
Recovery of an arenavirus entirely from RNA polymerase I/II-driven cDNA.
AuthorsFlatz L; Bergthaler A; de la Torre JC; Pinschewer DD
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID
The prototypic arenavirus lymphocytic choriomeningitis virus has been a primary workhorse of viral immunologists for almost a century, and it has served as an important model for studying basic principles of arenavirus molecular biology. Its negative-stranded bisegmented RNA genome has, however, posed a major obstacle to attempts at manipulating the ... More
Mechanical strain regulates syndecan-4 expression and shedding in smooth muscle cells through differential activation of MAP kinase signaling pathways.
AuthorsJulien MA; Wang P; Haller CA; Wen J; Chaikof EL
JournalAmerican Journal of Physiology. Cell Physiology
PubMed ID
Syndecan-4 (S4) belongs to a family of transmembrane proteoglycans, acts as a coreceptor for growth factor binding as well as cell-matrix and cell-cell interactions, and is induced in neointimal smooth muscle cells (SMCs) after balloon catheter injury. We investigated S4 expression in SMCs in response to several force profiles and ... More
Aberrant expression of VEGF-C is related to grade of cervical intraepithelial neoplasia (CIN) and high risk HPV, but does not predict virus clearance after treatment of CIN or prognosis of cervical cancer.
AuthorsProvost E; Hersperger G; Timmons L; Ho WQ; Hersperger E; Alcazar R; Shearn A
JournalJournal of Clinical Pathology
PubMed ID
AIMS: Increased angiogenesis leads to invasion in cervical cancer. Vascular endothelial growth factors (VEGFs) are involved in angiogenesis, but molecular links to the most important aetiological agent, human papillomavirus (HPV), need clarifying. MATERIAL/METHODS: Archival samples-150 squamous cell carcinomas (SCCs) and 152 cervical intraepithelial neoplasia (CIN) lesions-were examined immunohistochemically for anti-VEGF-C ... More
DNA sequencing by MALDI-TOF MS using alkali cleavage of RNA/DNA chimeras.
Approaches developed for sequencing DNA with detection by mass spectrometry use strategies that deviate from the Sanger-type methods. Procedures demonstrated so far used the sequence specificity of RNA endonucleases, as unfortunately equivalent enzymes for DNA do not exist and therefore require transcription of DNA into RNA prior to fragmentation. We ... More
Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells.
AuthorsGee K; Angel JB; Ma W; Mishra S; Gajanayaka N; Parato K; Kumar A
JournalThe Journal of Biological Chemistry
PubMed ID
Human immunodeficiency virus (HIV)-Tat plays an important role in virus replication and in various aspects of host immune responses, including dysregulation of cytokine production. IL-10, an anti-inflammatory cytokine, is up-regulated during the course of HIV infection representing an important pathway by which HIV may induce immunodeficiency. Here we show that ... More
Coactivation of the N-terminal transactivation of mineralocorticoid receptor by Ubc9.
Molecular mechanisms underlying mineralocorticoid receptor (MR)-mediated gene expression are not fully understood. Various transcription factors are post-translationally modified by small ubiquitin-related modifier-1 (SUMO-1). We investigated the role of the SUMO-1-conjugating enzyme Ubc9 in MR transactivation. Yeast two-hybrid, GST-pulldown, and coimmunoprecipitation assays showed that Ubc9 interacted with N-terminal MR-(1-670). Endogenous Ubc9 ... More
CYP2A13: variable expression and role in human lung microsomal metabolic activation of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone.
AuthorsZhang X; D'Agostino J; Wu H; Zhang QY; von Weymarn L; Murphy SE; Ding X
JournalThe Journal of Pharmacology and Experimental Therapeutics
PubMed ID
CYP2A13 is the most efficient cytochrome P450 enzyme in the metabolic activation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a tobacco-specific lung carcinogen. The aims of this study were to determine the levels of CYP2A13 protein in human lung microsomes and to ascertain whether CYP2A13 plays any role in lung microsomal NNK metabolic activation. ... More
A global drug inhibition pattern for the human ATP-binding cassette transporter breast cancer resistance protein (ABCG2).
JournalThe Journal of Pharmacology and Experimental Therapeutics
PubMed ID
In this article, we explore the entire structural space of registered drugs to obtain a global model for the inhibition of the drug efflux transporter breast cancer resistance protein (BCRP; ABCG2). For this purpose, the inhibitory effect of 123 structurally diverse drugs and drug-like compounds on mitoxantrone efflux was studied ... More
Epigenetic status of an adenovirus type 12 transgenome upon long-term cultivation in hamster cells.
AuthorsHochstein N; Muiznieks I; Mangel L; Brondke H; Doerfler W
JournalJournal of Virology
PubMed ID
The epigenetic status of integrated adenovirus type 12 (Ad12) DNA in hamster cells cultivated for about 4 decades has been investigated. Cell line TR12, a fibroblastic revertant of the Ad12-transformed epitheloid hamster cell line T637 with 15 copies of integrated Ad12 DNA, carries one Ad12 DNA copy plus a 3.9-kbp ... More
Isolation of cDNAs for R2R3-MYB, bHLH and WDR transcriptional regulators and identification of c and ca mutations conferring white flowers in the Japanese morning glory.
AuthorsMorita Y; Saitoh M; Hoshino A; Nitasaka E; Iida S
JournalPlant & Cell Physiology
PubMed ID
The transcriptional regulators for anthocyanin biosynthesis include members of proteins containing an R2R3-MYB domain, a bHLH (basic helix-loop-helix) domain and conserved WD40 repeats (WDRs). Spacial and temporal expression of the structural genes encoding the enzymes for anthocyanin biosynthesis is thought to be determined by combinations of the R2R3-MYB, bHLH and ... More
p21 gene polymorphisms in systemic lupus erythematosus.
AuthorsKong EK; Chong WP; Wong WH; Lau CS; Chan TM; Ng PK; Song YQ; Mak W; Lau YL
JournalRheumatology (Oxford, England)
PubMed ID
OBJECTIVE: Cyclin-dependent kinase inhibitor 1A (p21) is a negative regulator in the cell cycle. Development of sex-linked lupus-like syndrome in p21-/- mice and reduced p21 gene expression in patients with systemic lupus erythematosus (SLE) compared with those in healthy controls suggested that p21 is a susceptibility gene of SLE. We ... More
Sequence variation in the T-cell epitopes of the Plasmodium falciparum circumsporozoite protein among field isolates is temporally stable: a 5-year longitudinal study in southern Vietnam.
AuthorsJalloh A; van Thien H; Ferreira MU; Ohashi J; Matsuoka H; Kanbe T; Kikuchi A; Kawamoto F
JournalJournal of Clinical Microbiology
PubMed ID
In an effort to decipher the nature and extent of antigen polymorphisms of malaria parasites in a setting where malaria is hypomesoendemic, we conducted a 5-year longitudinal study (1998 to 2003) by sequencing the Th2R and Th3R epitopes of the circumsporozoite protein (CSP) of 142 Plasmodium falciparum field isolates from ... More
Cooperative interactions between RUNX2 and homeodomain protein-binding sites are critical for the osteoblast-specific expression of the bone sialoprotein gene.
AuthorsRoca H; Phimphilai M; Gopalakrishnan R; Xiao G; Franceschi RT
JournalThe Journal of Biological Chemistry
PubMed ID
The bone sialoprotein (Bsp) gene provides an excellent model for studying mechanisms controlling osteoblast-specific gene expression. Although the RUNX2 transcription factor directly regulates many osteoblast-related genes, its function in Bsp expression remains uncertain. By using chromatin immunoprecipitation (ChIP) analysis in MC3T3-E1 (clone MC-4) preosteoblast cells, RUNX2 was shown to bind ... More
Development and validation of endogenous reference genes for expression profiling of medaka (Oryzias latipes) exposed to endocrine disrupting chemicals by quantitative real-time RT-PCR.
AuthorsZhang Z; Hu J
JournalToxicological Sciences : An Official Journal of the Society of Toxicology
PubMed ID
The quantitative real-time reverse transcription polymerase chain reaction (Q-RT-PCR) technique has been increasingly used in endocrine disrupting chemicals (EDCs) research. Usually, an appropriate endogenous control gene is critical for Q-RT-PCR to normalize the errors and sample-to-sample variations that occur in the course of tissue collection, RNA isolation, and RT-PCR. In ... More
Roles for the human ATP-dependent Lon protease in mitochondrial DNA maintenance.
AuthorsLu B; Yadav S; Shah PG; Liu T; Tian B; Pukszta S; Villaluna N; Kutejova E; Newlon CS; Santos JH; Suzuki CK
JournalThe Journal of Biological Chemistry
PubMed ID
Human mitochondrial Lon is an ATP-powered proteolytic machine that specifically binds to single-stranded G-rich DNA and RNA in vitro. However, it is unknown whether Lon binds mitochondrial DNA (mtDNA) in living cells or functions in mtDNA integrity. Here, we demonstrate that Lon interacts with the mitochondrial genome in cultured cells ... More
Association of the PDCD5 locus with lung cancer risk and prognosis in smokers.
AuthorsSpinola M; Meyer P; Kammerer S; Falvella FS; Boettger MB; Hoyal CR; Pignatiello C; Fischer R; Roth RB; Pastorino U; Haeussinger K; Nelson MR; Dierkesmann R; Dragani TA; Braun A
JournalJournal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
PubMed ID
PURPOSE: Whole-genome scan association analysis was carried out to identify genetic variants predictive of lung cancer risk in smokers and to confirm the identified variants in an independent sample. PATIENTS AND METHODS: A case-control study was performed using two pools consisting of DNA from 322 German smoking lung cancer patients ... More
High qualitative and quantitative conservation of alternative splicing in Caenorhabditis elegans and Caenorhabditis briggsae.
AuthorsRukov JL; Irimia M; Mork S; Lund VK; Vinther J; Arctander P
JournalMolecular Biology and Evolution
PubMed ID
Alternative splicing (AS) is an important contributor to proteome diversity and is regarded as an explanatory factor for the relatively low number of human genes compared with less complex animals. To assess the evolutionary conservation of AS and its developmental regulation, we have investigated the qualitative and quantitative expression of ... More
Haplotype analysis of CYP11A1 identifies promoter variants associated with breast cancer risk.
AuthorsYaspan BL; Breyer JP; Cai Q; Dai Q; Elmore JB; Amundson I; Bradley KM; Shu XO; Gao YT; Dupont WD; Zheng W; Smith JR
JournalCancer Research
PubMed ID
The CYP11A1 gene encodes the cholesterol side chain cleavage enzyme that catalyzes the initial and rate-limiting step of steroidogenesis. A large number of epidemiologic studies have implicated the duration and degree of endogenous estrogen exposure in the development of breast cancer in women. Here, we conduct a systematic investigation of ... More
Dual effect of APOBEC3G on Hepatitis B virus.
AuthorsNoguchi C; Hiraga N; Mori N; Tsuge M; Imamura M; Takahashi S; Fujimoto Y; Ochi H; Abe H; Maekawa T; Yatsuji H; Shirakawa K; Takaori-Kondo A; Chayama K
JournalThe Journal of General Virology
PubMed ID
G to A hypermutation of Hepatitis B virus (HBV) and retroviruses appears as a result of deamination activities of host APOBEC proteins and is thought to play a role in innate antiviral immunity. Alpha and gamma interferons (IFN-alpha and -gamma) have been reported to upregulate the transcription of APOBEC3G, which ... More
Cloning and functional characterization of human sodium-dependent organic anion transporter (SLC10A6).
We have cloned human sodium-dependent organic anion transporter (SOAT) cDNA, which consists of 1502 bp and encodes a 377-amino acid protein. SOAT shows 42% sequence identity to the ileal apical sodium-dependent bile acid transporter ASBT and 33% sequence identity to the hepatic Na(+)/taurocholate-cotransporting polypeptide NTCP. Immunoprecipitation of a SOAT-FLAG-tagged protein ... More
Dynamic regulation of miRNA expression in ordered stages of cellular development.
AuthorsNeilson JR; Zheng GX; Burge CB; Sharp PA
JournalGenes & Development
PubMed ID
Short RNA expression in several distinct stages of T-lymphocyte development was comprehensively profiled. The total number of microRNAs (miRNAs) expressed per cell at different stages of development varies over nearly an order of magnitude in parallel with changes in total cellular RNA content, suggesting that global miRNA levels are coregulated ... More
Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature.
AuthorsGudnason H; Dufva M; Bang DD; Wolff A
JournalNucleic Acids Research
PubMed ID
The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, preferential ... More
Phagocytosis of Borrelia burgdorferi, the Lyme disease spirochete, potentiates innate immune activation and induces apoptosis in human monocytes.
AuthorsCruz AR; Moore MW; La Vake CJ; Eggers CH; Salazar JC; Radolf JD
JournalInfection and Immunity
PubMed ID
We have previously demonstrated that phagocytosed Borrelia burgdorferi induces activation programs in human peripheral blood mononuclear cells that differ qualitatively and quantitatively from those evoked by equivalent lipoprotein-rich lysates. Here we report that ingested B. burgdorferi induces significantly greater transcription of proinflammatory cytokine genes than do lysates and that live ... More
Secreted frizzled-related protein 1 loss contributes to tumor phenotype of clear cell renal cell carcinoma.
AuthorsGumz ML; Zou H; Kreinest PA; Childs AC; Belmonte LS; LeGrand SN; Wu KJ; Luxon BA; Sinha M; Parker AS; Sun LZ; Ahlquist DA; Wood CG; Copland JA
JournalClinical Cancer Research : An Official Journal of the American Association for Cancer Research
PubMed ID
PURPOSE: Incidence and mortality rates for renal cell carcinoma (RCC) have been rising for decades. Unfortunately, the molecular events that support RCC carcinogenesis remain poorly understood. In an effort to gain a better understanding of signaling events in clear cell RCC (cRCC), we investigated the antitumor activity of secreted frizzled-related ... More
Class II cytokine receptor gene cluster is a major locus for hepatitis B persistence.
AuthorsFrodsham AJ; Zhang L; Dumpis U; Taib NA; Best S; Durham A; Hennig BJ; Hellier S; Knapp S; Wright M; Chiaramonte M; Bell JI; Graves M; Whittle HC; Thomas HC; Thursz MR; Hill AV
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID
Persistent hepatitis B virus infection is a major risk factor for hepatocellular carcinoma, the most frequent cancer in some developing countries. Up to 95% of those infected at birth and 15% of those infected after the neonatal period fail to clear hepatitis B virus, together resulting in approximately 350 million ... More
Recombinant adenoviral vectors can induce expression of p73 via the E4-orf6/7 protein.
AuthorsShapiro GS; Van Peursem C; Ornelles DA; Schaack J; DeGregori J
Journal
PubMed ID
'Despite the utility of recombinant adenoviral vectors in basic research, their therapeutic promise remains unfulfilled. Most engineered adenoviral vectors use a heterologous promoter to transcribe a foreign gene. We show that adenoviruses containing the cytomegalovirus immediate-early promoter induce the expression of the proapoptotic cellular protein TAp73 via the cyclin-dependent kinase-retinoblastoma ... More
Gastric mucosal hyperplasia via upregulation of gastrin induced by persistent activation of gastric innate immunity in major histocompatibility complex class II deficient mice.
AuthorsFukui T; Nishio A; Okazaki K; Uza N; Ueno S; Kido M; Inoue S; Kitamura H; Kiriya K; Ohashi S; Asada M; Tamaki H; Matsuura M; Kawasaki K; Suzuki K; Uchida K; Fukui H; Nakase H; Watanabe N; Chiba T
Journal
PubMed ID
'BACKGROUND AND AIMS: Major histocompatibility complex class II deficient (Aalpha0/0) mice have decreased CD4+ T cells, making them immunologically similar to patients with acquired immunodeficiency syndrome (AIDS). Both patients with AIDS and Aalpha0/0 mice have hypertrophic gastric folds. To clarify the mechanism of gastric mucosal hyperplasia, we investigated the pathophysiology ... More
Molecular diagnosis of canine visceral leishmaniasis: Identification of Leishmania species by PCR-RFLP and quantification of parasite DNA by real-time PCR
'The efficacies of polymerase chain reaction (PCR) procedures for the diagnosis of canine visceral leishmaniasis (CVL), and of PCR-restriction fragment length polymorphism (RFLP) analysis for the identification of Leishmania species, have been assessed. Quantitative real-time PCR employing a SYBR Green dye-based system was standardised for the quantification of Leishmania kDNA ... More
Extensive DNA methylation in normal colorectal mucosa in hyperplastic polyposis.
AuthorsMinoo P; Baker K; Goswami R; Chong G; Foulkes WD; Ruszkiewicz AR; Barker M; Buchanan D; Young J; Jass JR
Journal
PubMed ID
'BACKGROUND: Hyperplastic polyposis of the colorectum is a precancerous condition that has been linked with DNA methylation. The polyps in this condition have been distinguished from typical small hyperplastic polyps and renamed sessile serrated adenomas. Sessile serrated adenomas also occur sporadically and appear to be indistinguishable from their counterparts in ... More
Methylation status of fragile histidine triad (FHIT) gene and its clinical impact on prognosis of patients with myelodysplastic syndrome
'The fragile histidine triad (FHIT) gene plays an important role in anti-cancer and the abnormal methylation of FHIT gene is found in many carcinomas. The epigenetic changes of tumor suppressor genes (TSG) are now recognized as an abnormal mechanism contributing to the development of myelodysplastic syndrome (MDS). To clarify the ... More
Gene silencing of myostatin in differentiation of chicken embryonic myoblasts by small interfering RNA.
AuthorsSato F; Kurokawa M; Yamauchi N; Hattori MA
Journal
PubMed ID
'Myostatin (GDF-8) is known to negatively regulate skeletal muscle mass in myogenesis, but few studies have been conducted on the function of endogenous GDF-8 in primary myoblasts. The present study was performed to assess the function of GDF-8 by RNA interference using primary culture of chicken embryonic myoblasts in which ... More
Correlation between severity of mucopolysaccharidoses and combination of the residual enzyme activity and efficiency of glycosaminoglycan synthesis
'Aim: To develop a method for prediction of severity and clinical course of mucopolysaccharidoses (MPS), a group of inherited metabolic diseases. Methods: Various biochemical and clinical parameters (including estimation of the level of clinical severity, presence of specific mutations, residual enzyme activity, urinary glycosaminoglycan (GAG) excretion, storage of GAG in ... More
SREBP-1-independent regulation of lipogenic gene expression in adipocytes.
'Sterol regulatory element-binding protein (SREBP)-1c is now well established as a key transcription factor for the regulation of lipogenic enzyme genes such as FAS in hepatocytes. Meanwhile, the mechanisms of lipogenic gene regulation in adipocytes remain unclear. Here, we demonstrate that those in adipocytes are independent of SREBP-1c. In adipocytes, ... More
Characterization of a Nonclassical Class I MHC Gene in a Reptile, the Galapagos Marine Iguana (Amblyrhynchus cristatus)
AuthorsGlaberman, S; Du Pasquier, L; Caccone, A
Journal
PubMed ID
'Squamates are a diverse order of vertebrates, representing more than 7,000 species. Yet, descriptions of full-length major histocompatibility complex (MHC) genes in this group are nearly absent from the literature, while the number of MHC studies continues to rise in other vertebrate taxa. The lack of basic information about MHC ... More
Evidence for an ancient BRCA1 mutation in breast cancer patients of yoruban ancestry
'Background BRCA1 recurrent mutations have rarely been assessed in non-founder populations. Still, identifying such mutations could be important for designing genetic testing strategies for high-risk breast/ovarian cancer families in non-founder populations. Objective To determine whether the recurrent BRCA1 Y101X mutation identified in Yoruban breast cancer patients represents a single historical ... More
Carbohydrate metabolism during prolonged exercise and recovery: interactions between pyruvate dehydrogenase, fatty acids, and amino acids.
AuthorsMourtzakis M; Saltin B; Graham T; Pilegaard H
Journal
PubMed ID
'During prolonged exercise, carbohydrate oxidation may result from decreased pyruvate production and increased fatty acid supply and ultimately lead to reduced pyruvate dehydrogenase (PDH) activity. Pyruvate also interacts with the amino acids alanine, glutamine, and glutamate, whereby the decline in pyruvate production could affect tricarboxycylic acid cycle flux as well ... More
Efficient Renal Recruitment of Macrophages and T Cells in Mice Lacking the Duffy Antigen/Receptor for Chemokines
'The Duffy antigen/receptor for chemokines (DARC) is a chemokine-binding protein that is expressed on erythrocytes and renal endothelial cells. DARC-mediated endothelial transcytosis of chemokines may facilitate the renal recruitment of macrophages and T cells, as has been suggested for neutrophils. We studied the role of Darc in two mouse models ... More
Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR
AuthorsRoberts, I; Ng, G; Foster, N; Stanley, M; Herdman, MT; Pett, MR; Teschendorff, A; Coleman, N
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'Background: Human papilloma virus (HPV) load and physical status are considered useful parameters for clinical evaluation of cervical squamous cell neoplasia. However, the errors implicit in HPV gene quantification by PCR are not well documented. We have undertaken the first rigorous evaluation of the errors that can be expected when ... More
Novel epigenetic mechanisms that control pluripotency and quiescence of adult bone marrow-derived Oct4(+) very small embryonic-like stem cells
AuthorsShin, DM; Zuba-Surma, EK; Wu, W; Ratajczak, J; Wysoczynski, M; Ratajczak, MZ; Kucia, M
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'Recently, we identified in adult tissues a population of Oct4(+) SSEA-1(+) Sca-1(+) lin(-)CD45(-) very small embryonic-like stem cells (VSELs). First, to address recent controversies on Oct4 expression in cells isolated from adult organs, we show here evidence that Oct4 promoter in bone marrow (BM)-derived VSELs has an open chromatin structure ... More
Lactobacilli stimulate the innate immune response and modulate the TLR expression of HT29 intestinal epithelial cells in vitro
'The potentially probiotic strain Lactobacillus plantarum BFE 1685 isolated from a child''s faeces and the probiotic strain Lactobacillus rhamnosus GG were investigated for their capability to influence the innate immune response of HT29 intestinal epithelial cells towards Salmonella enterica serovar Typhimurium. Furthermore, their capacity to modulate toll-like receptor expression of ... More
Telomere length measurement by a novel monochrome multiplex quantitative PCR method
AuthorsCawthon, RM
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'The current quantitative polymerase chain reaction (QPCR) assay of telomere length measures telomere (T) signals in experimental DNA samples in one set of reaction wells, and single copy gene (S) signals in separate wells, in comparison to a reference DNA, to yield relative T/S ratios that are proportional to average ... More
Expression of the mef(E) gene encoding the macrolide efflux pump protein increases in Streptococcus pneumoniae with increasing resistance to macrolides.
AuthorsWierzbowski AK; Boyd D; Mulvey M; Hoban DJ; Zhanel GG
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'Active macrolide efflux is a major mechanism of macrolide resistance in Streptococcus pneumoniae in many parts of the world, especially North America. In Canada, this active macrolide efflux in S. pneumoniae is predominantly due to acquisition of the mef(E) gene. In the present study, we assessed the mef(E) gene sequence ... More
RpoH mediates the expression of some, but not all, genes induced in Neisseria gonorrhoeae adherent to epithelial cells.
AuthorsDu Y; Arvidson CG
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'Neisseria gonorrhoeae (gonococcus [GC]), is highly adapted to the human host, the only known reservoir for gonococcal infection. However, since it is sexually transmitted, infection of a new host likely requires a regulatory response on the part of the gonococcus to respond to this significant change in environment. We previously ... More
Molecular basis of uropathogenic Escherichia coli evasion of the innate immune response in the bladder
AuthorsBillips, BK; Schaeffer, AJ; Klumpp, DJ
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'In the urinary tract, the innate immune system detects conserved bacterial components and responds to infection by activating the proinflammatory transcription factor NF-κB, resulting in cytokine secretion and neutrophil recruitment. Uropathogenic Escherichia coli (UPEC), however, has been shown to evade the host innate immune response by suppressing NF-κB activation in ... More
Epigenetic dysregulation of the Wnt signalling pathway in chronic lymphocytic leukaemia
AuthorsChim, CS; Pang, R; Liang, R
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'Background: Wnt signalling has recently been implicated in the pathogenesis of cancer. Methods: This study investigated the activity of Wnt signalling in peripheral blood chronic lymphocytic leukaemia (CLL) lymphocytes, and the methylation status of seven soluble Wnt antagonist genes, including WIF1, DKK3, APC, SFRP1, SFRP2, SFRP4 and SFRP5, by ... More
Analogs of GnRH-I and GnRH-II inhibit epidermal growth factor-induced signal transduction and resensitize resistant human breast cancer cells to 4OH-tamoxifen.
'About 50-64% of human breast cancers express receptors for GnRH-I. Direct antiproliferative effects of analogs of GnRH-I on human breast cancer cell lines have been shown. They are at least in part mediated by antagonizing growth promoting effects of estradiol, epidermal growth factor (EGF) or insulin-like growth factor. Recently, expression ... More
Associations of Ki-ras proto-oncogene mutation and p53 gene overexpression in sporadic colorectal adenomas with demographic and clinicopathologic characteristics.
AuthorsEinspahr JG; Martinez ME; Jiang R; Hsu CH; Rashid A; Bhattacharrya AK; Ahnen DJ; Jacobs ET; Houlihan PS; Webb CR; Alberts DS; Hamilton SR
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'In colorectal tumorigenesis, Ki-ras proto-oncogene mutation often occurs early in the adenoma-adenocarcinoma sequence, whereas mutation of the p53 gene is associated with late progression to carcinoma. We evaluated the relationship of demographic and clinicopathologic characteristics to Ki-ras mutation and p53 gene product overexpression in 1,093 baseline sporadic colorectal adenomas from ... More
Estradiol induces expression of 5-hydroxytryptamine (5-HT) 4, 5-HT5, and 5-HT6 receptor messenger ribonucleic acid in rat anterior pituitary cell aggregates and allows prolactin release via the 5-HT4 receptor.
AuthorsPapageorgiou A; Denef C
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'Serotonin [5-hydroxytryptamine (5-HT)] is known to control prolactin (PRL) release at a hypothalamic level, but a pituitary site of action remains poorly studied. The present study explores the acute effect of 5-HT on PRL release in rat anterior pituitary aggregate cell cultures, the influence of steroid and thyroid hormones, and ... More
Frequent HCV reinfection and superinfection in a cohort of injecting drug users in Amsterdam
Authorsvan de Laar, TJW; Molenkamp, R; van den Berg, C; Schinkel, J; Beld, MGHM; Prins, M; Coutinho, RA; Bruisten, SM
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'Background/Aims:This study investigates the occurrence of HCV reinfection and superinfection among HCV seroconverters participating in the Amsterdam Cohort Studies among drug users from 1985 through 2005. Methods: HCV seroconverters (n = 59) were tested for HCV RNA at five different time points: the last visit before seroconversion (t = ... More
PIK3CA mutation in colorectal cancer: Relationship with genetic and epigenetic alterations
AuthorsOgino, S; Kawasaki, T; Ohnishi, M; Suemoto, Y; Kirkner, GJ; Zepf, D; Yan, LY; Longtine, A; Fuchs, CS; Ogino, S
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'Somatic PIK3CA mutations are often present in colorectal cancer. Mutant PIK3CA activates AKT signaling, which up-regulates fatty acid synthase (FASN). Microsatellite instability (MSI) and CpG island methylator phenotype ( CIMP) are important molecular classifiers in colorectal cancer. However, the relationship between PIK3CA mutation, MSI and CIMP remains uncertain. Using Pyrosequencing ... More
Involvement of gene polymorphisms of the folate pathway enzymes in gene expression and anticancer drug sensitivity using the NCI-60 panel as a model
'Folate, a vitamin of the B group involved in one-carbon group metabolism, plays an important role in DNA synthesis and methylation. Several polymorphisms in the genes involved in folate uptake and biotransformations have been shown to be associated to the risk of cancer and to anticancer drug response. We studied ... More
The human brain endothelial cell line hCMEC/D3 as a human blood-brain barrier model for drug transport studies
'The human brain endothelial capillary cell line hCMEC/D3 has been developed recently as a model for the human blood-brain barrier. In this study a further characterization of this model was performed with special emphasis on permeability properties and active drug transport. Para- or transcellular permeabilities (P(e)) of inulin (0.74 x ... More
Overcoming multidrug resistance in human carcinoma cells by an antisense oligodeoxynucleotide-doxorubicin conjugate in vitro and in vivo
AuthorsRen, YH; Wang, Y; Zhang, YF; Wei, DZ
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'Multidrug resistance (MDR), a major obstacle to successful cancer chemotherapy, may be induced by amplification of the MDR1 gene and overexpression of the P-glycoprotein (P-gp), which acts as drug efflux pump decreasing intracellular drug accumulation. In this study, an antisense oligodeoxynucleotide--doxorubicin conjugate was used to overcome MDR in a human ... More
Prevalence and prognostic impact of NPM1 mutations in 1485 adult patients with acute myeloid leukemia (AML).
AuthorsThiede C; Koch S; Creutzig E; Steudel C; Illmer T; Schaich M; Ehninger G
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'Mutations of the nucleophosmin (NPM1) gene have recently been described in patients with acute myeloid leukemia (AML). To clarify the prevalence as well as the clinical impact of this mutation, we investigated 1485 patients with AML for NPM1 exon 12 mutations using fragment analysis. A 4 bp insert was detected ... More
Association of CA repeat polymorphism at intron 1 of insulin-like growth factor (IGF-I) gene with circulating IGF-I concentration, growth, and fatness in swine.
AuthorsEstany J; Tor M; Villalba D; Bosch L; Gallardo D; Jimenez N; Altet L; Noguera JL; Reixach J; Amills M; Sanchez A
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'Evidence is accumulating that intronic polymorphic cytosine-adenosine (CA) repeats may play a role in gene expression. In this work, we investigated whether a polymorphic CA short tandem repeat (STR) located at the first intron of the pig insulin-like growth factor I (IGF-I) gene influences plasma IGF-I concentration in pigs as ... More
Exon skipping mutations in collagen VI are common and are predictive for severity and inheritance
'Mutations in the genes encoding collagen VI (COL6A1, COL6A2, and COL6A3) cause Bethlem myopathy (BM) and Ullrich congenital muscular dystrophy (UCMD), two related conditions of differing severity. BM is a relatively mild dominantly inherited disorder characterized by proximal weakness and distal joint contractures. UCMD was originally regarded as an exclusively ... More
Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP)
AuthorsChen, JH; Lu, F; Lim, CS; Kim, JY; Ahn, HJ; Suh, IB; Takeo, S; Tsuboi, T; Sattabongkot, J; Han, ET
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'Loop-mediated isothermal amplification (LAMP) is a novel technique that rapidly amplifies target DNA in isothermal conditions. In a previous study, the sensitivities and specificities of LAMP microscopy, and nested PCR were compared in the context of rapid malaria detection. In the present study, LAMP detected vivax malaria parasites in 115 ... More
Postmortem Detection of Hepatitis B, C, and Human Immunodeficiency Virus Genomes in Blood Samples from Drug-Related Deaths in Denmark*
'Blood-borne viral infections are widespread among injecting drug users; however, it is difficult to include these patients in serological surveys. Therefore, we developed a national surveillance program based on postmortem testing of persons whose deaths were drug related. Blood collected at autopsy was tested for anti-HBc, anti-HBs, anti-hepatits C virus ... More
Mycobacterial infection induces the secretion of high-mobility group box 1 protein
'High-mobility group box protein 1 (HMGB1) is a non-histone nuclear protein that acts as a pro-inflammatory cytokine and is released by monocytes and macrophages. Necrotic cells also release HMGB1 at the site of tissue damage which induces a variety of cellular responses, including the expression of pro-inflammatory mediators. This study ... More
Intracellular-co-localization of trypsin-2 and matrix metalloprotease-9: Possible proteolytic cascade of trypsin-2, MMP-9 and enterokinase in carcinoma
AuthorsVilen, ST; Nyberg, P; Hukkanen, M; Sutinen, M; Ylipalosaari, M; Bjartell, A; Paju, A; Haaparanta, V; Stenman, UH; Sorsa, T; Salo, T
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'Tumor-associated trypsin-2 and matrix metalloprotease-9 (MMP-9) are associated with cancer, particularly with invasive squamous cell carcinomas. They require activation for catalytical competence via proteolytic cascades. One cascade is formed by enterokinase, trypsin-2 and MMP-9; enterokinase activates trypsinogen-2 to trypsin-2, which is an efficient proMMP-9 activator. We describe here that oral ... More
The molecular mechanism of peptide-mediated erythromycin resistance.
AuthorsLovmar M; Nilsson K; Vimberg V; Tenson T; Nervall M; Ehrenberg M
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'The macrolide antibiotic erythromycin binds at the entrance of the nascent peptide exit tunnel of the large ribosomal subunit and blocks synthesis of peptides longer than between six and eight amino acids. Expression of a short open reading frame in 23 S rRNA encoding five amino acids confers resistance to ... More
Bortezomib inhibits PKR-like endoplasmic reticulum (ER) kinase and induces apoptosis via ER stress in human pancreatic cancer cells.
AuthorsNawrocki ST; Carew JS; Dunner K Jr; Boise LH; Chiao PJ; Huang P; Abbruzzese JL; McConkey DJ
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'Bortezomib (Velcade, formerly known as PS-341) is a boronic acid dipeptide derivative that is a selective and potent inhibitor of the proteasome. We hypothesized that proteasome inhibition would lead to an accumulation of misfolded proteins in the cell resulting in endoplasmic reticulum (ER) stress. The ability of bortezomib to induce ... More
17q21.31 microduplication patients are characterised by behavioural problems and poor social interaction
'Background: Microdeletions at 17q21.31 have recently been shown to cause a novel syndrome. Here we identify the reciprocal 17q21.31 duplication syndrome in 4 patients. Method: Patients with the 17q21.31 duplication were identified by screening a large cohort of patients (n=13 070) with mental retardation and congenital malformation by comparative ... More
Reduced expression of vascular endothelial growth factor paralleled with the increased angiostatin expression resulting from the upregulated activities of matrix metalloproteinase-2 and -9 in human type 2 diabetic arterial vasculature.
'Impaired angiogenesis could contribute to the increased incidence of coronary and peripheral artery disease in diabetic patients. Angiogenesis is initiated by vascular endothelial growth factor (VEGF), a potent angiogenic cytokine, and suppressed by angiostatin, which is generated by matrix metalloproteinase (MMP)-2 and -9 through proteolytic cleavage of plasminogen. We hypothesized ... More
'BACKGROUND: Studies have shown that chronic stress or UV radiation independently suppress immunity. Given their increasing prevalence, it is important to understand whether and how chronic stress and UV radiation may act together to increase susceptibility to disease. Therefore, we investigated potential mediators of a stress-induced increase in emergence and ... More
Allele dropout in PCR-based diagnosis of Wilson disease: mechanisms and solutions.
AuthorsLam CW; Mak CM
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'BACKGROUND: We investigated the mechanisms leading to allele dropout-the nonamplification of 1 of the alleles-in PCR-based diagnosis of Wilson disease (WD). METHODS: We extracted genomic DNA from blood samples from 6 WD patients (P1-P6) with allele dropouts detected in a previous study of WD in a Hong Kong Chinese population. ... More
Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes.
AuthorsCivitarese AE; Hesselink MK; Russell AP; Ravussin E; Schrauwen P
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'Ingestion of carbohydrate during exercise may blunt the stimulation of fat oxidative pathways by raising plasma insulin and glucose concentrations and lowering plasma free fatty acid (FFA) levels, thereby causing a marked shift in substrate oxidation. We investigated the effects of a single 2-h bout of moderate-intensity exercise on the ... More
Promoter hypermethylation of the ADAM23 gene in colorectal cancer cell lines and cancer tissues
AuthorsChoi, JS; Kim, KH; Jeon, YK; Kim, SH; Jang, SG; Ku, JL; Park, JG
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'Promoter hypermethylation of the ADAM23 gene, which is normally involved in cell-to-cell and cell-to matrix adhesion, has been reported in pancreatic, breast and brain cancer, and recently the role of this gene was examined in gastric cancer. In this study, we analyzed ADAM23 expression in colorectal cancer cell lines and ... More
Functional RET G691S polymorphism in cutaneous malignant melanoma
'RET proto-oncogene encodes a receptor tyrosine kinase whose ligand is glial cell line-derived neurotrophic factor (GDNF), and its polymorphism at G691S juxtamembrane region (RETp) is a germline polymorphism. Cutaneous melanomas, particularly the desmoplastic subtype, are highly neurotropic; thus we sought to determine the frequency of RETp in cutaneous melanoma and ... More
Genome-wide identification of quantitative trait loci in a cross between Hampshire and Landrace II: Meat quality traits
AuthorsMarkljung, E; Braunschweig, MH; Karlskov-Mortensen, P; Bruun, CS; Sawera, M; Cho, IC; Hedebro-Velander, I; Josell, A; Lundstroem, K; von Seth, G; Jorgensen, CB; Fredholm, M; Andersson, L
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'Background: Meat quality traits are important in pig breeding programs, but they are difficult to include in a traditional selection program. Marker assisted selection (MAS) of meat quality traits is therefore of interest in breeding programs and a Quantitative Trait Locus (QTL) analysis is the key to identifying markers that ... More
A molecular phylogeny of fleas (Insecta: Siphonaptera): origins and host associations
AuthorsWhiting, MF; Whiting, AS; Hastriter, MW; Dittmar, K
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'Siphonaptera (fleas) is a highly specialized order of holometabolous insects comprising ∼2500 species placed in 16 families. Despite a long history of extensive work on flea classification and biology, phylogenetic relationships among fleas are virtually unknown. We present the first formal analysis of flea relationships based on a molecular matrix ... More
Neutralization of granulocyte macrophage colony-stimulating factor decreases amyloid beta 1-42 and suppresses microglial activity in a transgenic mouse model of Alzheimer's disease
AuthorsManczak, M; Mao, PZ; Nakamura, K; Bebbington, C; Park, B; Reddy, PH
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'The purpose of our study was to investigate microglia and astrocytes that are associated with human mutant amyloid precursor protein and amyloid beta (Aβ). We investigated whether the anti-granulocyte-macrophage-colony stimulating factor (GM-CSF) antibody can suppress microglial activity and decrease Aβ production in Alzheimer's disease transgenic mice (Tg2576 line). An antibody ... More
Multiple recurrent genetic events converge on control of histone lysine methylation in medulloblastoma
'We used high-resolution SNP genotyping to identify regions of genomic gain and loss in the genomes of 212 medulloblastomas, malignant pediatric brain tumors. We found focal amplifications of 15 known oncogenes and focal deletions of 20 known tumor suppressor genes (TSG), most not previously implicated in medulloblastoma. Notably, we identified ... More
Down-regulation of CD20 expression in B-cell lymphoma cells after treatment with rituximab-containing combination chemotherapies: its prevalence and clinical significance
AuthorsHiraga, J; Tomita, A; Sugimoto, T; Shimada, K; Ito, M; Nakamura, S; Kiyoi, H; Kinoshita, T; Naoe, T
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'Although rituximab is a key molecular targeting drug for CD20-positive B-cell lymphomas, resistance to rituximab has recently been recognized as a considerable problem. Here, we report that a CD20-negative phenotypic change after chemotherapies with rituximab occurs in a certain number of CD20-positive B-cell lymphoma patients. For 5 years, 124 patients ... More
Optimal Detection of Sentinel Lymph Node Metastases by Intraoperative Radioactive Threshold and Molecular Analysis in Patients with Melanoma
AuthorsManca, G; Romanini, A; Pellegrino, D; Borso, E; Rondini, M; Orlandini, C; Zucchi, V; Pasqualetti, F; Mariani, G
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'The aim of this study was to optimize a protocol for radioguided biopsy of the sentinel lymph node (SLN) in patients with melanoma. The protocol was based on a combination of ex vivo counting of the nodes detected intraoperatively and analysis of the harvested nodes by hematoxylin and eosin staining ... More
Expression of the tumour suppressor gene CADM1 is associated with favourable outcome and inhibits cell survival in neuroblastoma
'Cell adhesion molecule 1 (CADM1) is a putative tumour suppressor gene, which is downregulated in many solid tumours. In neuroblastoma, loss of CADM1 expression has recently been found in disseminated tumours with adverse outcome, prompting us to investigate its role in neuroblastoma tumour progression. Oligonucleotide-microarray analysis of 251 neuroblastoma specimens ... More
Histidine decarboxylase, DOPA decarboxylase, and vesicular monoamine transporter 2 expression in neuroendocrine tumors: immunohistochemical study and gene expression analysis.
AuthorsUccella S; Cerutti R; Vigetti D; Furlan D; Oldrini R; Carnevali I; Pelosi G; La Rosa S; Passi A; Capella C
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'Histidine decarboxylase (HDC) and vesicular monoamine transporter 2 (v-MAT2) are involved in the biosynthesis and storage of histamine. DOPA decarboxylase (DDC) is involved in the biosynthesis of a variety of amines and shares a high degree of homology with HDC. HDC and v-MAT2 immunoreactivities (IR) have recently been detected in ... More
Association of 5-HTT gene polymorphism, platelet MAO activity, and drive for thinness in a population-based sample of adolescent girls
AuthorsAkkermann, K; Paaver, M; Nordquist, N; Oreland, L; Harro, J
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'Objective: Several lines of evidence suggest that alterations in serotonergic activity contribute to the pathophysiology of abnormal eating behaviors. Since platelet monoamine oxidase (MAO) activity and the 5-HT transporter gene promoter polymorphism (5-HTTLPR) have been associated with eating disorders, the knowledge from a population-based sample may provide useful information which ... More